Mineral composition, content of phenolic compounds and in vitro antioxidant and antibacterial activities of aqueous and organic extracts of the seeds of Peganum harmala L.

Senhaji, Souad; Lamchouri, Fatima; Boulfia, Mohamed; Lachkar, Nacima; Bouabid, Khadija; Toufik, Hamid

doi:10.1016/j.sajb.2022.03.005

“…With this in mind, we concluded that phenolic compounds were key players in free radical scavenging and reducing power assays. These findings are consistent with those reported in the literature, where a positive correlation between total phenol and radical scavengers and reducing abilities has been reported [ 17 , 18 ]. The phosphomolybdenum assay also included the conversion of Mo (VI) to Mo (V) by antioxidants at acidic condition.…”

Section: Resultssupporting

confidence: 93%

“Smart Extraction Chain” with Green Solvents: Extraction of Bioactive Compounds from Picea abies Bark Waste for Pharmaceutical, Nutraceutical and Cosmetic Uses

Sut

¹

,

Maccari

²

,

Zengin

³

et al. 2022

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Secondary metabolites from the sawmill waste Picea abies bark were extracted using an innovative two-step extraction that includes a first step with supercritical CO2 (SCO2) and a second step using green solvents, namely ethanol, water, and water ethanol mixture. Maceration (M), ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE) techniques were applied in the second step. A total of nineteen extract were obtained and yield were compared. Bark extracts were characterized by LC-DAD-MSn and classes of compounds were quantified as abietane derivatives, piceasides, flavonoids, and phenolics to compare different extractions. Obtained extracts were studied by in vitro assay to evaluate potential pharmaceutical, nutraceutical and cosmetic uses assessing the antioxidant activity as well as the inhibitory activity on target enzymes. Results show that the “smart extraction chain” is advantageous in term of yield of extraction and phytoconstituent concentration. SCO2 extract, presenting a unique composition with a large amount of abietane derivatives, exerted the best activity for amylase inhibition compared to the other extracts.

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“…In this study, the percentage of inhibition is 87.5% against Botrytis cinerea and 96.47% against Oidium oxysporum. The analysis performed by Senhaji et al, [28] revealed that the extraction by infusion of Peganum harmala brought out few flavonoids 8.65±0.35 mg GAE / mg E which may explain why these compounds did not appear in our analyses. This extract is also a great source of alkaloids as confirmed by Lamchouri et al, [22,23,24] .…”

Section: Fungal/fungistatic Effectmentioning

confidence: 71%

“…While the appearance of flavonoids is mentioned by the same source and also by Ouassou et al [26], wich is confirmed by correlation. The variation in extracts composition is linked to several factors such as the extraction methods, plant parts and genotypes, geographical origin and environment conditions, the harvesting period, the degree of drying, and drying conditions [27,28].…”

Section: Fungal/fungistatic Effectmentioning

confidence: 99%

Antifungal activity of plant extracts against tomato’s fungal diseases

Abbad

¹

,

Aouji²,

Zelmat³

et al. 2023

E3S Web Conf.

1

0

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Fungal diseases have always been a major problem for tomato crops. Growers generally use chemical fungicides to treat this type of diseases. However, these products are toxic to the environment and the consumer, especially if the pre-harvest interval is not respected. The present study aims to find non-polluting alternatives. Five plant extracts (Peganum harmala, Ocimum basilicum, Caralluma europaea, Nerium oleander and Eucalyptus globulus) are tested for their in vitro efficiency against four pathogenic fungi: Alternaria solani, Botrytis cinerea, Botrytis cinerea taken from fruit, Phytophthora infestans and Oïdium oxysporum. The obtained results reveal that the extract of Ocimum basilicum is the most effective on the studied fungi. Indeed, at a concentration of 0.4%, it inhibited at 80% the development of Botrytis cinerea and at 81% Oïdium oxysporum at a concentration of 0.2%. Followed by Peganum harmala and Nerium oleander, which also showed an antifungal effect (Peganum harmala inhibited up to 73% of the growth of Alternaria solani at a dose of 4%). The extracts of Caralluma europaea and Eucalyptus globulus proved similar antifungal activity, which exceeded 30%. The study of the fungal/fungistatic effect revealed that all the studied extracts have a fungal effect against the treated fungi. The phytochemical screening showed that the plants extracts are rich in polyphenols especially Ocimum basilicum, Peganum harmala and Nerium oleander. This leads us to deduce that the antifungal activity may be due to this.

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“…The quantitative analysis of mineral elements was done according to the protocol of Arora and collaborators [42]. The test procedure has been described in detail in our previous work [43][44][45].…”

Section: Mineralogical Studymentioning

confidence: 99%

Ethnopharmacological Survey, Mineral and Chemical Content, In Vitro Antioxidant, and Antibacterial Activities of Aqueous and Organic Extracts of Chamaerops humilis L. var. argentea Andre Leaves

Lachkar

¹

,

Lamchouri

²

,

Toufik

³

2022

BioMed Research International

Self Cite

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Introduction. The present study is carried out for the first time on Chamaerops humilis L. var. argentea Andre from the region of Taza using an ethnopharmacological survey, an experimental study of the mineralogical and chemical compositions, and evaluations of the antioxidant and antibacterial activities. Methods. After conducting the ethnopharmacological survey, a mineralogical and phytochemical study involving the preparation of aqueous and organic extracts was done. Essential oils were also extracted by hydrodistillation. Subsequently, qualitative and quantitative chemical analyses were performed. In vitro evaluation of antioxidant activities was performed by five tests (H2O2, DPPH, ABTS, FRAP, and RP) and antibacterial activities by the disc method and determination of MIC and MBC. A principal component analysis (PCA) was performed to visualize the different correlations. Results. The different parts of the plant are used for the treatment of digestive disorders, cardiovascular diseases, and diabetes. In addition, the leaves are rich in mineral compounds, catechic tannins, flavonoids, and sterols. However, they have some traces of essential oils. The quantitative analysis revealed that the ethanolic macerated had a higher content of total polyphenols ( 100.27 ± 1.95 mg EAG/g E) and catechic tannins ( 52.11 ± 1.02 mg EC/g E). This extract had a strong antioxidant capacity (H2O2 ( 37.34 ± 0.55 % ), DPPH ( I C 50 = 31.18 ± 0.66 μ g / ml ), ABTS ( 108.28 ± 1.29 mg E AA/g E), FRAP ( 148.85 ± 0.43 mg E T/g E), and RP ( 10.86 ± 0.01 mg E AA/g E). The same extract had a bactericidal effect against Staphylococcus aureus. Principal component analysis (PCA) showed that antioxidant activity was highly correlated with the chemical composition of C. humilis leaves; a high correlation was recorded between the total polyphenol content and ABTS ( r = 0.9779 ), FRAP ( r = 0.9644 ), DPPH ( r = 0.9418 ), and PR ( r = 0.9271 ) tests. In addition, cathectic tannins were highly correlated with the tests of DPPH ( r = 0.9753 ) and ABTS ( r = 0.8843 ). Flavonoids were similarly correlated with DPPH ( r = 0.8897 ) and ABTS ( r = 0.7599 ) tests. Conclusion. These results could justify the traditional use of the leaves of Chamaerops humilis in the region of Taza for the treatment of some diseases.

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Mineral composition, content of phenolic compounds and in vitro antioxidant and antibacterial activities of aqueous and organic extracts of the seeds of Peganum harmala L.

Cited by 13 publications

References 64 publications

“Smart Extraction Chain” with Green Solvents: Extraction of Bioactive Compounds from Picea abies Bark Waste for Pharmaceutical, Nutraceutical and Cosmetic Uses

“Smart Extraction Chain” with Green Solvents: Extraction of Bioactive Compounds from Picea abies Bark Waste for Pharmaceutical, Nutraceutical and Cosmetic Uses

Antifungal activity of plant extracts against tomato’s fungal diseases

Ethnopharmacological Survey, Mineral and Chemical Content, In Vitro Antioxidant, and Antibacterial Activities of Aqueous and Organic Extracts of Chamaerops humilis L. var. argentea Andre Leaves

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