Mineral apposition rates provide significant information on long‐term effects in BMP‐induced bone regeneration

Abstract

In this study, a CaP biomaterial was used as a carrier for rhBMP-2. Biomaterials were investigated in calvarial and femoral defects using a rabbit animal model, with unloaded biomaterials serving as control. Fluorochrome labels were administered at days 14 and 70. Specimens were retrieved after 12 weeks for histological analysis. When area fractions were assessed by conventional histomorphometry, no significant effect of rhBMP-2 on the amounts of regenerated bone and residual biomaterial were seen by 12 weeks. After mineral appositional rate (MAR) measurement using double labels, calculation yielded significantly higher MARs for defects at both implantation sites, when compared with surrounding bone, whether or not biomaterials were loaded with rhBMP-2. Analyzing the effect of rhBMP-2, both defect sites showed significantly higher MARs in the rhBMP-2 group. MARs of bone surrounding the defects had also been elevated significantly by rhBMP-2 at calvarial and femoral implantation sites. It is concluded that MAR measurement is suitable to identify long-term effects of rhBMP-2 on bone formation at a time when conventional histomorphometry using fractional area determination is inadequate. Also, by MAR assessment, effects of rhBMP-2 on surrounding bone can be documented.

Repair of bone defects around dental implants with bone morphogenetic protein/fibroblast growth factor-loaded porous calcium phosphate cement: a pilot study in a canine model

“…Bone labeling data showed significantly elevated MAR values with BMP‐2/bFGF in the defects, as compared with BMP‐2 or bFGF. The MAR value for BMP‐2 of 1.8 μm/day in this study is close to the result in the study by Schopper et al (2009). The benefits of MAR assessment were shown in the histomorphometrical investigation of bone regeneration using a biomaterial loaded with BMP‐2/bFGF.…”

“…In clinical practice, a mineral apposition rate (MAR) measurement is frequently used for diagnostic classification and treatment evaluation of various metabolic bone diseases. MAR values are also assessed to quantify the effects of growth factors in Ca–P biomaterial research (Schopper et al 2009). We utilized fluorescence observation of undecalcified sections to demonstrate the feasibility of using the CPC‐BMP‐2/bFGF combination in order to induce bone formation.…”

Vinyl esters: Low cytotoxicity monomers for the fabrication of biocompatible 3D scaffolds by lithography based additive manufacturing

“…As an established animal model in biomaterial research, adult female New Zealand white rabbits were used for in vivo testing of polymers based on vinyl esters 38. Scaffolds of 5 × 5 × 8 mm 3 containing a 3D‐microstructure were fabricated (Fig.…”

Utilization and evaluation of CHO‐specific sequence databases for mass spectrometry based proteomics

“…For preliminary in vivo testing these 3D cellular structures of 5 × 5 × 8 mm were implanted into surgical defects of the distal femoral bone of adult New Zealand white rabbits under general anesthesia [Figs 6(b,c)] 37…”