Mimosa caesalpiniifolia rhizobial isolates from different origins of the Brazilian Northeast

Martins, Paulo Geovani Silva; Lira, M. A.; Fracetto, Giselle Gomes Monteiro; Silva, Maria Luiza Ribeiro Bastos da; Vincentin, Rayssa Pereira; Lyra, Maria do Carmo Catanho Pereira de

doi:10.1007/s00203-014-1078-8

Cited by 15 publications

(16 citation statements)

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“…The results suggest that "mulungu" shows a preference for nodulation with α-rhizobia rather than β-rhizobia, though few isolates were evaluated. Among the eight bacteria assessed, only one isolate was classified as a β-proteobacteria, which are isolates commonly obtained from Mimosoidae legumes Gyaneshwar et al, 2011;Martins et al, 2015). These data are in agreement with our hypothesis that the bacterial isolates studied here belong to different taxonomical clusters.…”

Section: Discussionsupporting

confidence: 91%

“…Low genetic similarity of new β-rhizobia from the region and species already described was recently shown by Martins et al (2015), who obtained Paraburkholderia isolates from Mimosa caesalpiniifolia in the Brazilian semi-arid region, and analysis of 16S rRNA sequences showed that some isolates had low similarity with β-rhizobia described. Other taxonomical studies are needed to achieve better taxonomic positioning of ESA 71.…”

Section: Discussionmentioning

confidence: 71%

“…The high similarity of ESA 71 to the Bcc bacteria indicates that, in spite of already being described as indigenous to the Caatinga (Reis Júnior et al, 2010; Martins et al, 2015), the native β-rhizobia in the region should belong to a new species, different from those already described worldwide. Low genetic similarity of new β-rhizobia from the region and species already described was recently shown by Martins et al (2015), who obtained Paraburkholderia isolates from Mimosa caesalpiniifolia in the Brazilian semi-arid region, and analysis of 16S rRNA sequences showed that some isolates had low similarity with β-rhizobia described.…”

Section: Discussionmentioning

confidence: 91%

“…Research efforts in recent years have been applied to evaluate the diversity of bacterial isolates obtained from native legumes to improve our understanding of rhizobia diversity in the "Caatinga" (Teixeira et al, 2010;Freitas et al, 2014;Martins et al, 2015;Menezes et al, 2016). The results indicated large diversity among the rhizobia inhabiting the root nodules of the legume species already evaluated.…”

Section: Introductionmentioning

confidence: 99%

“…Plants belonging to the Mimosa genus are the best studied among "Caatinga" native legumes, and advances in the taxonomy of their rhizobia have shown the prevalence of β-rhizobia, mainly Paraburkholderia spp. (formerly Burkholderia) (Reis Júnior et al, 2010;Martins et al, 2015). Regarding Papilionoidae tree legumes, results achieved around the world have indicated the prevalence of α-rhizobia inside their root nodules, although β-rhizobia have also already been observed with lower frequency (Rasolomampianina et al, 2005;Elliott et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil

Menezes

Escobar

Fraiz

et al. 2017

Rev. Bras. Ciênc. Solo

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Legume-rhizobia symbiosis is a cross-kingdom association that results in large amounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic efficiency of eight rhizobial isolates obtained from "mulungu" (Erythrina velutina Willd.) nodules. For 16S rRNA gene sequencing, the genomic DNA was extracted using a commercial kit, amplified with universal primers, and subjected to sequencing reactions. For the isolate ESA 71, PCR amplifications for nodC and nodA genes were attempted. Rhizobial efficiency was assessed by two greenhouse experiments. The first assay was carried out under gnotobiotic conditions, with sterile sand as a substrate; the second experiment was conducted in a non-sterile soil. For both experiments, the inoculation treatments consisted of a single inoculation of each isolate, in addition to a treatment with Bradyrhizobium elkanii BR 5609 as a reference strain. Furthermore, two non-inoculated control treatments, supplied and not supplied with mineral N, were also evaluated. Bacterial identification indicated that both α and β-rhizobia could be found in "mulungu" root nodules. Three isolates where classified within the Rhizobium genus, four bacteria belonged to Bradyrhizobium and one isolate clustered with Burkholderia. Positive amplification of an intragenic fragment of the nodA gene using a primer set to β-rhizobia could be found for ESA 71 (Burkholderia). All bacterial isolates were effective in colonizing "mulungu" roots. In the first experiment, all inoculated treatments and N fertilization increased the N concentration in "mulungu" shoot tissues. For total N in the shoots, the isolates ESA 70, ESA 72, and ESA 75 stood out. In the non-sterile substrate experiment, the isolates ESA 70, ESA 71, ESA 72, and ESA 75, together with the reference strains, induced increases in the shoot N concentration and total accumulation compared to the absolute control. The results indicate that "mulungu" is able to establish associations with efficient α and β-rhizobia in Brazilian semi-arid soils.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 71%