Mimicking the physical cues of the ECM in angiogenic biomaterials

Crosby, Cody; Zoldan, Janet

doi:10.1093/rb/rbz003

Cited by 62 publications

(56 citation statements)

References 108 publications

(118 reference statements)

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“…It is likely that biglycan and LOXL-2 contributed to such protective effects. Sprouting angiogenesis is related to extensive ECM remodeling (Neve et al, 2014;Crosby and Zoldan, 2019). Multiple levels of cell-ECM interactions are potentially involved in capillary formation (Edgar et al, 2014;Rauff et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles From Notch Activated Cardiac Mesenchymal Stem Cells Promote Myocyte Proliferation and Neovasculogenesis

Wang

Khan

Ashraf

2020

Front. Cell Dev. Biol.

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Cardiac mesenchymal stem cells (C-MSCs) are a novel mesenchymal stem cell (MSC) subpopulation derived from cardiac tissue, which are reported to be responsible for cardiac regeneration. Notch signaling is believed to aid in cardiac repair following myocardial injury. In this study, we have investigated the role of extracellular vesicles (EVs) from Notch1 engineered C-MSCs on angiogenesis and cardiomyocyte (CM) proliferation in ischemic myocardium. C-MSCs were isolated from Notch1 flox mice (C-MSC Notch1FF). Notch1 gene deletion was accomplished by adenoviral vectormediated Cre recombination, and Notch1 overexpression was achieved by overexpression of Notch1 intracellular domain (N1ICD). EVs were isolated by using the size exclusion column method. Proteomic composition of EV was carried out by mass spectrometry. A mouse myocardial infarction (MI) model was generated by permanent left anterior descending (LAD) coronary artery ligation. Intramyocardial transplantation of Notch1 knockout C-MSCs (C-MSCs Notch1KO) did not have any effect on cardiac function and scar size. On the other hand, transplantation of N1ICD-overexpressing C-MSCs (C-MSCs N1ICD) showed significant improvement in cardiac function and attenuation of fibrosis as compared to the control (PBS) group and non-modified C-MSC groups. C-MSCs N1ICD differentiated into smooth muscle cells and formed new vessels. Proteomics profiling identified several proteins, such as lysyl oxidase homolog-2 and biglycan, as highly enriched proteins in EV-C-MSCs N1ICD. Go term analysis indicated that EV-C-MSCs N1ICD were enriched with bioactive factors, potent prorepair proteins responsible for cell migration and proliferation. EV-C-MSCs Notch1FF and EV-C-MSCs N1ICD were strongly proangiogenic under both in vitro and in vivo conditions. EV-C-MSCs N1ICD caused dense tube formation in vitro and increased neovasculogenesis in the peri-infarct area in vivo. Furthermore, EV-C-MSCs N1ICD attenuated endothelial cell (EC) and CM apoptosis under oxidative stress and ischemic injury. Similarly, EV-C-MSC Notch1FF and EV-C-MSC N1ICD treatment improved cardiac

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Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles From Notch Activated Cardiac Mesenchymal Stem Cells Promote Myocyte Proliferation and Neovasculogenesis

Wang

Khan

Ashraf

2020

Front. Cell Dev. Biol.

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“…In light of the above, biomaterials for cardiac patches must be biocompatible and must provide the correct mechanical stiffness, matrix density, and viscoelasticity to induce angiogenesis together with the appropriate biological clues (Crosby and Zoldan, 2019 ). In this regard, ELR/ELP-based scaffolds can be created with tunable mechanical properties that can be adjusted on-demand, depending on the final application, simply by varying the final concentration and/or amino acid composition of the polypeptides.…”

Section: Cardiac Muscle Regeneration and Angiogenesismentioning

confidence: 99%

Elastin-Based Materials: Promising Candidates for Cardiac Tissue Regeneration

Torre

Alonso

Rodríguez-Cabello

2020

Front. Bioeng. Biotechnol.

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Stroke and cardiovascular episodes are still some of the most common diseases worldwide, causing millions of deaths and costing billions of Euros to healthcare systems. The use of new biomaterials with enhanced biological and physical properties has opened the door to new approaches in cardiovascular applications. Elastin-based materials are biomaterials with some of the most promising properties. Indeed, these biomaterials have started to yield good results in cardiovascular and angiogenesis applications. In this review, we explore the latest trends in elastin-derived materials for cardiac regeneration and the different possibilities that are being explored by researchers to regenerate an infarcted muscle and restore its normal function. Elastin-based materials can be processed in different manners to create injectable systems or hydrogel scaffolds that can be applied by simple injection or as patches to cover the damaged area and regenerate it. Such materials have been applied to directly regenerate the damaged cardiac muscle and to create complex structures, such as heart valves or new bio-stents that could help to restore the normal function of the heart or to minimize damage after a stroke. We will discuss the possibilities that elastin-based materials offer in cardiac tissue engineering, either alone or in combination with other biomaterials, in order to illustrate the wide range of options that are being explored. Moreover, although tremendous advances have been achieved with such elastin-based materials, there is still room for new approaches that could trigger advances in cardiac tissue regeneration.

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“…There are five groups of MMPs and they are produced as pro-enzymes which must be proteolytically processed to be activated. The zinc that is present in the endopeptidases, is the site where other enzymes can disrupt the MMPs to activate them [ 5 , 8 ].…”

Section: Extracellular Matrixmentioning

confidence: 99%

Angiogenic Potential in Biological Hydrogels

Giraudo

Francesco

Catoira³

et al. 2020

Biomedicines

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Hydrogels are three-dimensional (3D) materials able to absorb and retain water in large amounts while maintaining their structural stability. Due to their considerable biocompatibility and similarity with the body’s tissues, hydrogels are one of the most promising groups of biomaterials. The main application of these hydrogels is in regenerative medicine, in which they allow the formation of an environment suitable for cell differentiation and growth. Deriving from these hydrogels, it is, therefore, possible to obtain bioactive materials that can regenerate tissues. Because vessels guarantee the right amount of oxygen and nutrients but also assure the elimination of waste products, angiogenesis is one of the processes at the base of the regeneration of a tissue. On the other hand, it is a very complex mechanism and the parameters to consider are several. Indeed, the factors and the cells involved in this process are numerous and, for this reason, it has been a challenge to recreate a biomaterial able to adequately sustain the angiogenic process. However, in this review the focal point is the application of natural hydrogels in angiogenesis enhancing and their potential to guide this process.

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Mimicking the physical cues of the ECM in angiogenic biomaterials

Cited by 62 publications

References 108 publications

Extracellular Vesicles From Notch Activated Cardiac Mesenchymal Stem Cells Promote Myocyte Proliferation and Neovasculogenesis

Extracellular Vesicles From Notch Activated Cardiac Mesenchymal Stem Cells Promote Myocyte Proliferation and Neovasculogenesis

Elastin-Based Materials: Promising Candidates for Cardiac Tissue Regeneration

Angiogenic Potential in Biological Hydrogels

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