Mimicking physiological O2 tension in the female reproductive tract improves assisted reproduction outcomes in pig

García-Martínez, Soledad; Hurtado, Miguel; Gutierrez, Hugo Andres; Margallo, Francisco Miguel Sánchez; Romar, Raquel; Latorre, Rafael; Coy, Pilar; López-Albors, Octavio

doi:10.1093/molehr/gay008

Cited by 38 publications

(33 citation statements)

References 42 publications

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“…Thus, we assume this was the reason for the higher pregnancy and parturition outcomes obtained here. Also the use of a minimally invasive procedure based on a LESS approach might have in uenced positively the pregnancy rate because, as demonstrated in previous works [29,30], the trauma to the uterus was minimal and the full recovery of the animal after procedure signi cantly reduced compared to laparotomy. Despite this, the percentage of non-pregnant animals after embryo transfer in our study was still higher than 64% in both groups.…”

Section: Discussionmentioning

confidence: 98%

Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant placental expression of PEG3 and LUM.

París-Oller

Navarro-Serna

Soriano‐Úbeda

et al. 2020

Preprint

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Background: In vitro embryo production (IVP) and embryo transfer (ET) are two very common assisted reproductive technologies (ART) in human and cattle. However, in pig, the combination of either procedures, or even their use separately, is still considered suboptimal due to the low efficiency of IVP plus the difficulty of performing ET in the long and contorted uterus of the sow. In addition, the potential impact of these two ART on the health of the offspring is unknown. We investigated here if the use of an improved IVP system, with natural reproductive fluids (RF) as supplements to the culture media, combined with a minimal invasive surgery to perform ET, affects the output of the own IVP system as well as the reproductive performance of the mother and placental molecular traits.Results: The blastocyst rates obtained by both in vitro systems, conventional (C-IVP) and improved (RF-IVP), were similar. Pregnancy and farrowing rates were also similar. However, when compared to in vivo control (artificial insemination, AI), litter sizes of both IVP groups were lower, while placental efficiency was higher in AI than in RF-IVP. Gene expression studies revealed aberrant expression levels for PEG3 and LUM in placental tissue for C-IVP group when compared to AI, but not for RF-IVP group. Conclusions: The use of reproductive fluids as additives for the culture media in pig IVP does not improve reproductive performance of recipient mothers but can mitigate the impact of artificial procedures in the offspring.

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Section: Discussionmentioning

confidence: 98%

Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant placental expression of PEG3 and LUM.

París-Oller

Navarro-Serna

Soriano‐Úbeda

et al. 2020

Preprint

Self Cite

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“…However, most of the genes belonging to the former ontology group were upregulated after the 7 days days of culture, when compared with the first day. This may be due to the cell exposure to atmospheric oxygen tension, which is much higher than in oviduct, and may cause excessive ROS accumulation in culture medium in vitro [26].…”

Section: Discussionmentioning

confidence: 99%

Genes regulating biochemical pathways of oxygen metabolism in porcine oviductal epithelial cells during long-term IVC

Kocherova

Brązert

Sujka-Kordowska

et al. 2019

Medical Journal of Cell Biology

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Oxygen metabolism has an important role in the normal functioning of reproductive system, as well as the pathogenesis of female infertility. Oxidative stress seems to be responsible for the initiation or development of reproductive organ diseases, including polycystic ovary syndrome, endometriosis, preeclampsia, etc. Given the important role of maintaining balance between the production of ROS and antioxidant defence in the proper functioning of reproductive system, in the present study we aimed to analyse the expression of genes related to oxygen metabolism in porcine oviductal epithelial cells during long-term in vitro culture. The oviducts were collected from 45 crossbred gilts at the age of approximately nine months that displayed at least two regular oestrous cycles. The oviductal endothelial cells were isolated by enzymatic digestion to establish long-term primary cultures. Gene expression changes between 7, 15 and 30 daysof culturewere analysed with the use ofwhole transcriptome profiling by Affymetrix microarrays. The most of the "cellular response to oxidative stress" genes were upregulated. However, we did not observe any main trend in changes within the "cellular response to oxygen-containing compound" ontology group, where the gene expression levels were changed in various manner.Running title: Oxygen metabolism in porcine oviductal epithelial cells

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“…After fertilization, the embryos are cultured at 38.5°C in a humidified atmosphere of 5% CO 2 in air overnight, and then the next day (Day 1), they are moved to a humidified atmosphere of low oxygen (5% O 2 , 5% CO 2 , and 90% N 2 ) until Day 6 (Chen et al, ). Recently, García‐Martínez et al () demonstrated that the use of the same O 2 conditions as those found in the pig reproductive tract (7% O 2 during IVF and embryo culture) were beneficial for embryo production in vitro.…”

Section: Transcriptomic and Metabolomic Approaches To Understand Preimentioning

confidence: 99%

“…Recently, García-Martínez et al (2018) demonstrated that the use of the same O 2 conditions as those found in the pig reproductive tract (7% O 2 during IVF and embryo culture) were beneficial for embryo production in vitro.…”

Section: Transcriptomic and Metabolomic Approaches To Understand Prmentioning

confidence: 99%

Applications of omics and nanotechnology to improve pig embryo production in vitro

Lucas

Chen

Seixas

et al. 2019

Molecular Reproduction Devel

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An appropriate environment to optimize porcine preimplantation embryo production in vitro is required as genetically modified pigs have become indispensable for biomedical research and agriculture. To provide suitable culture conditions, omics technologies have been applied to elucidate which metabolic substrates and pathways are involved during early developmental processes. Metabolomic profiling and transcriptional analysis comparing in vivo‐ and in vitro‐derived embryos have demonstrated the important role of amino acids during preimplantation development. Transcriptional profiling studies have been helpful in assessing epigenetic reprogramming agents to allow for the correction of gene expression during the cloning process. Along with this, nanotechnology, which is a highly promising field, has allowed for the use of engineered nanoplatforms in reproductive biology. A growing number of studies have explored the use of nanoengineered materials for sorting, labeling, and targeting purposes; which demonstrates their potential to become one of the solutions for precise delivery of molecules into gametes and embryos. Considering the contributions of omics and the recent progress in nanoscience, in this review, we focused on their emerging applications for current in vitro pig embryo production systems to optimize the generation of genetically modified animals.

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Mimicking physiological O2 tension in the female reproductive tract improves assisted reproduction outcomes in pig

Abstract: This study was funded by Spanish Ministry of Economy and Competitiveness (MINECO) and European Regional Development Fund (FEDER). Grants AGL2012-40180-C03-01 and AGL2015-66341-R. The authors declare no conflict of interest.

Cited by 38 publications

References 42 publications

Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant placental expression of PEG3 and LUM.

Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant placental expression of PEG3 and LUM.

Genes regulating biochemical pathways of oxygen metabolism in porcine oviductal epithelial cells during long-term IVC

Applications of omics and nanotechnology to improve pig embryo production in vitro

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