Mimetic peptide and special antibody: promising agents for optimizing hapten-analyzing systems

Li, Ping; Deng, Shengliang

doi:10.1039/c5ay02533h

Cited by 6 publications

(4 citation statements)

References 77 publications

(71 reference statements)

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“…However, in any case the detection sensitivity depends strongly on the affinity of the antibody used and the differential interactions between separated V H and V L chains in the presence or absence of the target. Moreover, as only one antibody is involved, OS-IA can suffer from cross-reactivity [84,85]. Suzuki et al [86] developed an OS-IA for the detection of ZEN by using the split Fvs (V H and V L chains) of a monoclonal anti-ZEN in phage display or fusion protein formats.…”

Section: Recombinant Antibodiesmentioning

confidence: 99%

Bioinspired recognition elements for mycotoxin sensors

2017

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Mycotoxins are low molecular weight molecules produced as secondary metabolites by filamentous fungi that can be found as natural contaminants in many foods and feeds. These toxins have been shown to have adverse effects on both human and animal health, and are the cause of significant economic losses worldwide. Sensors for mycotoxin analysis have traditionally applied elements of biological origin for the selective recognition purposes. However, since the 1970s there has been an exponential growth in the use of genetically engineered or synthetic biomimetic recognition elements that allow some of the limitations associated with the use of natural receptors for the analyses of these toxins to be circumvented. This review provides an overview of recent advances in the application of bioinspired recognition elements, including recombinant antibodies, peptides, aptamers, and molecularly imprinted polymers, to the development of sensors for mycotoxins based on different transduction elements. Graphical abstract Novel analytical methods based on bioinspired recognition elements, such as recombinant antibodies, peptides, aptamers, and molecularly imprinted polymers, can improve the detection of mycotoxins and provide better tools than their natural counterparts to ensure food safety.

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Section: Recombinant Antibodiesmentioning

confidence: 99%

Bioinspired recognition elements for mycotoxin sensors

2017

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“…Although the developed immunoassay methods are excellent, they are used for detection of ZEN involving the use of ZEN itself, including the free form and the bound form (Li & Deng, 2016; Zhou, Liu, et al, 2021). Mycotoxin standards are also expensive.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence immunoassay based on phage mimotope for nontoxic detection of Zearalenone in maize

Zhou

Wang

et al. 2022

Journal of Food Safety

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Zearalenone (ZEN) is one of the most common mycotoxin contaminants worldwide. In this study, a phage-based direct competitive fluorescence immunosorbent assay (P-dcFLISA) was developed for the detection of ZEN. In this P-dcFLISA, phage mimotope was used to replace chemically synthesized antigens to improve the safety of experiments. Furthermore, ZnCdSe/ZnS (core/shell) quantum dots (QDs) as fluorescent labeling material replaced chromogenic substrate, which are used in traditional ELISA. The 50% inhibition value (IC 50 ) of P-dcFLISA was 0.301 ng/ml, which was approximately 12-fold lower than that of phage-based indirect competitive enzyme-linked immunosorbent assay (P-icELISA). The limit of detection (LOD) of P-dcFLISA was 0.023 ng/ml and the detection range was 0.060-1.531 ng/ml. In the added recovery assay, recovery rate was 93.18-105.52%, with the coefficient variation (CV) ranging from 8.39% to 10.55%. The establishment of P-icFLISA not only save time, but also avoid the use of mycotoxins in the detection process, providing a safe method for the detection of mycotoxins. In conclusion, phage-based fluorescence immunoassay method has great potential application in ZEN detection and agricultural monitoring.

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“…Therefore, competing antigens or alternatively competing tracers should be introduced for mycotoxin immunoassays. 24 The competing reagents are always prepared by conjugating mycotoxins or their derivatives with carrier proteins through chemical synthesis, which showed the drawbacks of complex procedures, prolonged reaction periods, significant batch errors, and high costs. 25 In addition, the chemical binding efficiency is low because of bridge interference and many unnecessary cross reactions.…”

Section: ■ Introductionmentioning

confidence: 99%

“…These assays are usually performed in the competitive format because mycotoxins are too small to be captured by two antibodies. Therefore, competing antigens or alternatively competing tracers should be introduced for mycotoxin immunoassays . The competing reagents are always prepared by conjugating mycotoxins or their derivatives with carrier proteins through chemical synthesis, which showed the drawbacks of complex procedures, prolonged reaction periods, significant batch errors, and high costs .…”

Section: Introductionmentioning

confidence: 99%

Mimotope-Based Immunoassays for the Rapid Analysis of Mycotoxin: A Review

Huang

et al. 2021

J. Agric. Food Chem.

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Mycotoxins are toxic contaminants in foods and feeds that are naturally occurring and largely unavoidable. Determining their contents in these products is essential to protect humans from harm. Immunoassays of mycotoxins have been well-established because they are fast, sensitive, simple, and cost-effective. However, a major limitation of immunoassays is the requirement of toxic mycotoxins as competing antigens, standards, or competing tracers. Mimotopes are peptides or proteins that can specifically bind to antibodies and compete with analytes for binding sites by mimicking antigenic epitopes. They can be employed as substitutes for competing antigens, standards, or competing tracers to avoid use of mycotoxins. This review summarizes the production and functionalization of the two main kinds of mimotopes, mimic peptides and anti-idiotypic antibodies (Ab2), and their applications in rapid analysis of mycotoxins.

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Mimetic peptide and special antibody: promising agents for optimizing hapten-analyzing systems

Abstract: Immunoassays are simple, rapid, and cost-effective detection systems that are widely used for the detection of haptens in varied matrices.

Cited by 6 publications

References 77 publications

Bioinspired recognition elements for mycotoxin sensors

Bioinspired recognition elements for mycotoxin sensors

Fluorescence immunoassay based on phage mimotope for nontoxic detection of Zearalenone in maize

Mimotope-Based Immunoassays for the Rapid Analysis of Mycotoxin: A Review

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