Miltenberger blood group typing by real‐time polymerase chain reaction (<scp>qPCR</scp>) melting curve analysis in Thai population

Vongsakulyanon, Apirom; Kitpoka, Pimpun; Kunakorn, Mongkol; Srikhirin, Toemsak

doi:10.1111/tme.12265

Cited by 7 publications

(5 citation statements)

References 19 publications

(24 reference statements)

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“…Vongsakulyanon and colleagues developed a quantitative PCR high‐resolution melting (HRM) curve analysis to genotype hybrid glycophorins; however, multiple pairs of primers for two HRM protocols and subsequent steps were needed to distinguish GYP(B‐A‐B) genotypes. A similar HRM genotyping assay for GYP(B‐A‐B) was recently described requiring only one primer set in a single PCR HRM setup .…”

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confidence: 99%

Genotyping analysis of MNS blood group GP(B‐A‐B) hybrid glycophorins in the Chinese Southern Han population using a high‐resolution melting assay

et al. 2018

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confidence: 99%

Genotyping analysis of MNS blood group GP(B‐A‐B) hybrid glycophorins in the Chinese Southern Han population using a high‐resolution melting assay

et al. 2018

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“…In this article, molecular test result was not included. However, the gDNA extracted from donors left over blood was successfully used for Miltenberger blood group typing [17]. Actually, we are working to reduce or eliminate sample collection cost.…”

Section: Concentration Yield and Purity Of Gdnamentioning

confidence: 99%

Donors left over blood as a source of genomic DNA for genetic studies

Hassan¹,

Rahman²,

Ahmad³

et al. 2016

cems

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Screening a mutation, polymorphism or genetic studies in a population involving a large sample size and costing. Left over blood from donors can be a genomic DNA (gDNA) source without collection cost. Thus, this article report the mean concentration, yield and purity of gDNA extracted from donors left over blood and the association of gDNA yield with age, gender and blood type of donors. Sample size was calculated using PS software

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“…Detection of MNS system glycophorin variant phenotypes is known to be limited to phenotyping reagents, which are not commercially available, and certainly genotyping assays, including polymerase chain reaction (PCR)-based deoxyribonucleic acid (DNA) typing, are applicable as reliable and reproducible alternatives. [ 11 12 13 14 15 21 22 23 24 25 26 27 ] In this study, the target DNA for GP. Mur, GP.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of GP. Mur variant phenotype among Malaysian blood donors

Rahman¹,

Hassan²,

Mohamad³

et al. 2023

Asian J Transfus Sci

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BACKGROUND AND OBJECTIVE: A number of glycophorin variant phenotypes or hybrid glycophorin variants of the MNS blood group system bear multiple immunogenic antigens such as Mi a , Mur, and MUT. In the East and Southeast Asian populations, glycoprotein (GP.) Mur is the most common glycophorin variant phenotype expressing those three immunogens. The aim of this study was to detect MNS system glycophorin variant phenotypes (GP. Mur, GP. Hop, GP. Bun, GP. HF, and GP. Hut) among Malaysian blood donors. MATERIALS AND METHODS: In this cross-sectional study, 144 blood donors were selected under stratified random sampling. The deoxyribonucleic acid was extracted from whole blood samples, followed by a polymerase chain reaction assay. Sanger sequencing was used to identify the specific MNS variants and then validated by a serological crossmatch with known anti-Mur and anti-MUT. RESULTS: GP. Mur was identified among Malaysian blood donors with a prevalence of 6.94%, and no other variants of the MNS system were found. CONCLUSION: The present study substantiates that GP. Mur is the main variant of the MNS system glycophorin (B-A-B) hybrid in Malaysian blood donors. GP. Mur-negative red blood cells must therefore be considered in the current transfusion policy in order to prevent alloimmunization and immune-mediated transfusion reactions, particularly in transfusion-dependent patients.

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Miltenberger blood group typing by real‐time polymerase chain reaction (qPCR) melting curve analysis in Thai population

Abstract: Mi(a) typing qPCR correctly identified Mi(a) blood groups in a Thai population with the feasibility of Mi(a) subtype discrimination, and Mi(a) subtyping qPCR was able to further define GP.Mur from other Mi(a) subtypes.

Cited by 7 publications

References 19 publications

Genotyping analysis of MNS blood group GP(B‐A‐B) hybrid glycophorins in the Chinese Southern Han population using a high‐resolution melting assay

Genotyping analysis of MNS blood group GP(B‐A‐B) hybrid glycophorins in the Chinese Southern Han population using a high‐resolution melting assay

Donors left over blood as a source of genomic DNA for genetic studies

Prevalence of GP. Mur variant phenotype among Malaysian blood donors

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