Milliwatt three- and four-pulse double electron electron resonance for protein structure determination

Teucher, Markus; Sidabras, Jason W.; Schnegg, Alexander

doi:10.1039/d1cp05508a

Cited by 3 publications

(1 citation statement)

References 70 publications

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“…[8] The modulation depth depends on the inversion efficiency 𝜆 and thus on the excitation bandwidth (i.e. the length, power, [117] and shape [118] ) of the microwave pulses in relation to the width of the EPR spectrum. In the case of biomolecular samples prepared by site-directed spin labelling, the labelling efficiency f needs to be taken into account, so that the modulation depth of a two-spin system is given by [40,119] Δ = 𝜆 ⋅ 𝑓 (91) If f = 1 and in the limit that the whole EPR spectrum is excited in a PDS experiment (𝜆 = 1),  equals 100%.…”

Section: Pulsed Dipolar Epr Spectroscopymentioning

confidence: 99%

PELDOR Measurements on Nitroxide-Labeled Oligonucleotides

Hett

Schiemann

2022

DNAzymes

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Most results presented in this thesis are the outcome of teamwork, and I would like to thank all my collaborators for their valuable contributions. In the project on time-resolved PELDOR, I had the pleasure to collaborate with Prof. Dr. U. Benjamin Kaupp and his group at the Max Planck Institute for Neurobiology of Behavior -caesar, Dr. Reinhard Seifert, Dr. Tobias Zbik, and Norbert Brenner. Furthermore, I would like to thank Prof. Dr. Helmut Grubmüller and Dr. Reinhard Klement from the Max Planck Institute for Multidisciplinary Sciences in Göttingen for introducing me to the field of molecular dynamics simulations and their helpful advice.The EPR measurements on trityls were done in collaboration with Dr. Nico Fleck, Jean-Jacques Jassoy, and Caspar Heubach and I would like to thank them for synthesizing the trityl spin labels and for labelling the proteins. Special thanks go to Dr. Dinar Abdullin for his advice on spectrometer operation and our enlightening discussions on various topics such as spin physics, programming, and data fitting. Likewise, I would like to thank Hamed Alaei for his technical support and for his cooperation in conducting the students' practical courses. Beyond, I would like to express my gratitude to all present and previous members of the research group for the nice working atmosphere and for fruitful discussions -especially my office mates, Catrin Allar, Olivia Kendall, Kevin Kopp, and Maria Vicino, shall be mentioned here.The computation centre of the University of Bonn is acknowledged for granting access to the Bonna cluster and the Deutsche Forschungsgemeinschaft (DFG, project number 420322655, Koselleck grant to U. B. Kaupp) for funding my research.Finally, I would like to thank my friends and family, especially my parents and my brother, for supporting me during my studies.

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Section: Pulsed Dipolar Epr Spectroscopymentioning

confidence: 99%

PELDOR Measurements on Nitroxide-Labeled Oligonucleotides

Hett

Schiemann

2022

DNAzymes

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Conducting a three-pulse DEER experiment without dead time: A review

Dzuba

2023

Journal of Magnetic Resonance Open

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Recent advances in microresonators and supporting instrumentation for electron paramagnetic resonance spectroscopy

Abhyankar

Agrawal

Campbell

et al. 2022

Review of Scientific Instruments

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Electron paramagnetic resonance (EPR) spectroscopy characterizes the magnetic properties of paramagnetic materials at the atomic and molecular levels. Resonators are an enabling technology of EPR spectroscopy. Microresonators, which are miniaturized versions of resonators, have advanced inductive-detection EPR spectroscopy of mass-limited samples. Here, we provide our perspective of the benefits and challenges associated with microresonator use for EPR spectroscopy. To begin, we classify the application space for microresonators and present the conceptual foundation for analysis of resonator sensitivity. We summarize previous work and provide insight into the design and fabrication of microresonators as well as detail the requirements and challenges that arise in incorporating microresonators into EPR spectrometer systems. Finally, we provide our perspective on current challenges and prospective fruitful directions.

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Milliwatt three- and four-pulse double electron electron resonance for protein structure determination

Abstract: Electron paramagnetic resonance (EPR) experiments for protein structure determination using double electron-electron resonance (DEER) spectroscopy rely on very high incident microwave powers (>300 W) to create the short pulse lengths...

Cited by 3 publications

References 70 publications

PELDOR Measurements on Nitroxide-Labeled Oligonucleotides

PELDOR Measurements on Nitroxide-Labeled Oligonucleotides

Conducting a three-pulse DEER experiment without dead time: A review

Recent advances in microresonators and supporting instrumentation for electron paramagnetic resonance spectroscopy

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