Milky spots in the mouse omentum may play an important role in the origin of peritoneal macrophages

Wijffels, J.F.A.M.; Hendrickx, Roeland J.B.M.; Steenbergen, Joke J. E.; Eestermans, I.L.; Beelen, R.H.J.

doi:10.1016/s0923-2494(05)80072-0

Cited by 74 publications

(61 citation statements)

References 13 publications

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“…Studded on the surface of the greater omental fold are numerous milky spots composed mainly of macrophages. 43,44 It has previously been demonstrated that upon experimental implantation of tumor cells into mice, tumor cells trafficked preferentially to and resided in the greater omentum. 45,46 Hence, it is possible that while tumors distributed in the peritoneal cavity are efficiently infected and destroyed by the viruses, tumor cells residing in the greater omentum are less accessible and thus not easily infected by the virus.…”

Section: Discussionmentioning

confidence: 99%

Oncolytic activities of approved mumps and measles vaccines for therapy of ovarian cancer

et al. 2005

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Section: Discussionmentioning

confidence: 99%

Oncolytic activities of approved mumps and measles vaccines for therapy of ovarian cancer

et al. 2005

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“…They have also been suggested to be very reactive perivascular tissue involved in different aspects of B and T-cell functions and are regarded as omental-associated lymphoid tissue (Beelen 1991). Immunophenotyping and precursor cell kinetic studies demonstrated that MS play a role in the origin of peritoneal macrophages in rats (Wijffels et al 1992) and that macrophages are the predominant cell type associated with these structures (Van Vugt et al 1993), suggesting that MS are involved in peritoneal antibacterial defence (Liebermann-Meffert & White 1983, Van Vugt et al 1996. In our studies, we proposed that MS (individual structures), considered as a whole, constitute an organised coelom-associated lymphomyelopoietic tissue (CALT), which represents the main immune system in peritoneal and other coelomic cavities (Lenzi et al 1996).…”

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confidence: 99%

Omental and pleural milky spots: different reactivity patterns in mice infected with Schistosoma mansoni reveals coelomic compartmentalisation

Panasco

Pelajo-Machado

Lenzi

2010

Mem. Inst. Oswaldo Cruz

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In vertebrate animals, pleural and peritoneal cavities are repositories of milky spots (MS), which constitute an organised coelom-associated lymphomyeloid tissue that is intensively activated by Schistosoma mansoni infection. This study compared the reactive patterns of peritoneal MS to pleural MS and concluded from histological analysis that they represent independent responsive compartments. Whole omentum, lungs and the entire mediastinum of 54 S. mansoni-infected mice were studied morphologically. The omental MS of infected animals were highly activated, modulating from myeloid-lymphocytic (60 days of infection) to lymphomyeloid (90 days of infection) and lymphocytic or lymphoplasmacytic (160 days of infection) types. The non-lymphoid component predominated in the acute phase of infection and was expressed by monocytopoietic, eosinopoietic and neutropoietic foci, with isolated megakaryocytes and small foci of late normoblasts and mast cells. Nevertheless, pleural or thoracic MS of infected mice were monotonous, consisting of small and medium lymphocytes with few mast and plasma cells and no myeloid component. Our data indicate that compartmentalisation of the MS response is dependent on the lymphatic vascularisation of each coelomic cavity, limiting the effects or consequences of any stimulating or aggressive agents, as is the case with S. mansoni infection

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“…Cell composition and functions of local defense spots in the peritoneal cavity have been studied in rats (Wijffels et al 1992;Van Vught et al 1996;Di Paolo & Sacchi 2000;Hekking et al 2001), mice (Dux 1986;Cranshaw & Leak 1990;Wijffels et al 1992) and humans (Shimotsuma et al 1991;Shimotsuma et al 1993;Takemori et al 1995;Krist et al 1997;Wilkosz et al 2005). However, to the best of our knowledge there is no published data on the local defense foci of the peritoneal cavity in birds.…”

Section: Groupmentioning

confidence: 99%

“…Milky spots have been reported to be abundantly present around the capillary of omentum, which is rich in blood vessels in rats (Wijffels et al 1992;Van Vugt et al 1996;Hekking et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Macrophages and lymphocytes are the principal cell types in the milky spots in non-stimulated mouse and can migrate from the interstitial spaces of milky spots into the peritoneal cavity (Wijffels et al 1992). The role of macrophages in the milky spots is clear microorganism and malignant cells from the peritoneal cavity (Dullens et al 1991;Hagiwara et al 1993).…”

Section: Introductionmentioning

confidence: 99%

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A comparative study of local defense spots within the peritoneal cavity in Japanese quails,Coturnix coturnix japonica(Galliformes, Phasianidae) and Wistar rats,Rattus norvegicus(Rodentia, Muridae)

Saydam

Eren

2012

Italian Journal of Zoology

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The local defense spots (milky spot) are found on the greater omentum but also in other peritoneal regions, as well as on the pleura and pericardium. Milky spots are small accumulations of leukocytes scattered around the capillaries. The most frequent cells in milky spots are the macrophages, followed by lymphocytes and mast cells. In this study, the structure of milky spots of quails and rats after antigenic stimulation with bovine serum albumin were examined at the light microscope levels by applying conventional histochemical and immunohistochemical methods. The parietal peritoneum and mesentery samples from quails and parietal peritoneum, mesentery and omentum majus samples from rats were collected. Local defense spots were seen as local lymphoid cell aggregations in the omentum, mesentery and parietal peritoneum in rats, whereas in quails they were seen only in the mesentery. After the bovine serum albumin injection, the dimensions of spots were at their biggest 6 hours after injection (p < 0.001) in the rat and 1 hour after injection (p < 0.01) in the quail. Lymphocytes, macrophages (MHC II positive cells in quail), plasma cells and mast cells were detected within the aggregates of both rat and quails. In conclusion, it was determined that the quails have local defense spots at the mesentery like the milky spots of rats. In addition, the present study findings confirm that local defense spots react to antigenic stimulation by innate and adaptive immunity in rats and quails.

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Milky spots in the mouse omentum may play an important role in the origin of peritoneal macrophages

Cited by 74 publications

References 13 publications

Oncolytic activities of approved mumps and measles vaccines for therapy of ovarian cancer

Oncolytic activities of approved mumps and measles vaccines for therapy of ovarian cancer

Omental and pleural milky spots: different reactivity patterns in mice infected with Schistosoma mansoni reveals coelomic compartmentalisation

A comparative study of local defense spots within the peritoneal cavity in Japanese quails,Coturnix coturnix japonica(Galliformes, Phasianidae) and Wistar rats,Rattus norvegicus(Rodentia, Muridae)

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