Milk phospholipid and plant sterol-dependent modulation of plasma lipids in healthy volunteers

Keller, Sylvia; Malarski, Angelika; Reuther, Carolin; Kertscher, Romy; Kiehntopf, Michael; Jahreis, Gerhard

doi:10.1007/s00394-012-0427-0

Cited by 28 publications

(18 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies carried out by Ohlsson et al [44,45] on humans, consisting of the supplementation of a sphingolipid-enriched dairy formulation, partially supported the findings that these molecules may affect cholesterol concentrations in TG-rich lipoproteins, but they did not find any effect on the level of plasma lipids or lipoproteins. Keller et al [46] concluded that milk PL supplementations influenced the plasma cholesterol amount, but did not change the LDL/HDL ratio.…”

Section: Biological Activity and Health Effectsmentioning

confidence: 99%

Phospholipids in Milk Fat: Composition, Biological and Technological Significance, and Analytical Strategies

Contarini

Povolo

2013

IJMS

254

178

View full text Add to dashboard Cite

Glycerophospholipids and sphingolipids are quantitatively the most important phospholipids (PLs) in milk. They are located on the milk fat globule membrane (MFGM) and in other membranous material of the skim milk phase. They include principally phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylserine, while sphingomyelin is the dominant species of sphingolipids There is considerable evidence that PLs have beneficial health effects, such as regulation of the inflammatory reactions, chemopreventive and chemotherapeutic activity on some types of cancer, and inhibition of the cholesterol absorption. PLs show good emulsifying properties and can be used as a delivery system for liposoluble constituents. Due to the amphiphilic characteristics of these molecules, their extraction, separation and detection are critical points in the analytical approach. The extraction by using chloroform and methanol, followed by the determination by high pressure liquid chromatography (HPLC), coupled with evaporative light scattering (ELSD) or mass detector (MS), are the most applied procedures for the PL evaluation. More recently, nuclear magnetic resonance spectrometry (NMR) was also used, but despite it demonstrating high sensitivity, it requires more studies to obtain accurate results. This review is focused on milk fat phospholipids; their composition, biological activity, technological properties, and significance in the structure of milk fat. Different analytical methodologies are also discussed.

show abstract

Section: Biological Activity and Health Effectsmentioning

confidence: 99%

Phospholipids in Milk Fat: Composition, Biological and Technological Significance, and Analytical Strategies

Contarini

Povolo

2013

IJMS

254

178

View full text Add to dashboard Cite

show abstract

“…Intake of 32.4 g lipamin M20 provided approximately 16.8 g protein (milk fat globule membrane fraction not specified) and 6 g PL to the body. Total plasma PL, phosphatidylcholine, sphingomyelin, and phosphatidyl inositol concentrations did not differ from baseline values or between groups after the interventions with lipamin M20 [103]. Following intake of 16.2 g lipamin 20, the plasma level of phosphatidyl ethanolamine was higher than at baseline.…”

Section: Phospholipidsmentioning

confidence: 80%

“…Total and HDL cholesterol levels in plasma were significantly lower after daily intake of 16.2 g lipamin 20 compared with levels at baseline, while total and LDL cholesterol levels following intake of 32.4 g lipamin 20 were significantly higher than levels after intake of 16.2 g lipamin 20. Stool weight was higher following daily intake of 6 g milk PL compared with baseline [103]. Fecal pH was significantly lower following the intervention than at baseline, and fecal weight of PL (mg/g dry matter) increased.…”

Section: Phospholipidsmentioning

confidence: 82%

“…They found no significant difference in plasma TG concentrations, but it seems to increase HDL cholesterol when compared with control who did not receive the sphingolipids within the milk formulation. Recently, Keller et al assessed the effects of lipamin M20 on plasma lipids [103]. Lipamin M20 is a milk phospholipid concentrate prepared from butter concentrate.…”

Section: Phospholipidsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Dairy Protein and Fat on the Metabolic Syndrome and Type 2 Diabetes

Bjørnshave¹,

Hermansen²

2014

Rev Diabet Stud

View full text Add to dashboard Cite

■ AbstractThe incidence of the metabolic syndrome (MetS) and type 2 diabetes (T2D) is increasing worldwide. Evidence supports a negative relationship between the consumption of dairy products and risk of MetS and T2D. Dairy proteins are known to have a directly beneficial effect on hypertension, dyslipidemia, and hyperglycemia, but a detailed understanding of the underlying mechanisms is missing. It has been confirmed by observations that the insulinotropic effect of dairy proteins is associated with the amino acid composition; in particular branched-chain amino acids (BCAA) seem to be of vital importance. Dairy protein-derived peptides may also contribute to the insulinotropic effect via dipeptidyl peptidase-4 (DPP-4) inhibitory activity, and may lower the blood pressure (BP). The lipid metabolism may be improved by whey protein (WP), which acts to reduce the postprandial triglyceride (TG) response. The effect of dairy fat is much more controversial because of the potentially harmful effect exerted by saturated fatty acid (SFA) on metabolic health. Recent observations suggest less adverse effects of SFA on metabolic health than previous assumed. However, little is known about dairy lipid fractions belonging to the groups of monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), and phospholipids (PL). Dairy fat seems to act differently depending on the dairy product and the composition of macronutrients in the meal. Therefore, for a better understanding of the mechanisms behind the dairy protein and fat effect on MetS, we suggest that more human studies should be carried out to clarify the interactions of dairy protein and fat with macronutrients in the meal and other dairy components, such as micronutrients and microorganisms from fermented products.Keywords: type 2 diabetes · metabolic syndrome · glucose metabolism · lipid metabolism · blood pressure · dairy fat

show abstract

“…Then organs were homogenized in homogenization buffer (0.25 M sucrose, 1 mM Na 2 EDTA, 10 mM HEPES, pH 7.0 including a protease inhibitor cocktail) by using a Potter-Elvehjem homogenizer. A small amount of every organ homogenate was saved for determination of the total a-Toc concentration and reference levels of proteins and PC [36]. The remaining homogenates were centrifuged for 10 Min at 750g and 4 8C.…”

Section: Lysosome Isolation By Ultracentrifugation From Mouse Organsmentioning

confidence: 99%

Quantification of age‐related changes of α‐tocopherol in lysosomal membranes in murine tissues and human fibroblasts

et al. 2016

View full text Add to dashboard Cite

Considering the biological function of α‐tocopherol (α‐Toc) as a potent protective factor against oxidative stress, this antioxidant is in the focus of aging research. To understand the role of α‐Toc during aging we investigated α‐Toc concentrations in young and aged primary human fibroblasts after supplementation with RRR‐α‐Toc. Additionally, α‐Toc contents were determined in brain, kidney, and liver tissue of 10 week‐, 18 month‐, and 24 month‐old mice, which were fed a standard diet containing 100 mg/kg dl‐α‐tocopheryl acetate. α‐Toc concentrations in isolated lysosomes and the expression of the α‐Toc transport proteins Niemann Pick C1 (NPC1), Niemann Pick C2 (NPC2), and lipoprotein lipase were also analyzed. Obtained data show a significant age‐related increase of α‐Toc in murine liver, kidney, and brain tissue as well as in human dermal fibroblasts. Also liver and kidney lysosomes are marked by elevated α‐Toc contents with aging. NPC1 and NPC2 protein amounts are significantly decreased in adult and aged murine kidney tissue. Also aged human dermal fibroblasts show decreased NPC1 amounts. Supplementation of young and aged fibroblasts led also to decreased NPC1 amounts, suggesting a direct role of this protein in α‐Toc distribution. Our results indicate an age‐dependent increase of α‐Toc in different murine tissues as well as in human fibroblasts. Furthermore saturation and intracellular distribution of α‐Toc seem to be strongly dependent on the availability of this vitamin as well as on the presence of the lysosomal protein NPC1. © 2016 BioFactors, 42(3):307–315, 2016

show abstract

Milk phospholipid and plant sterol-dependent modulation of plasma lipids in healthy volunteers

Cited by 28 publications

References 56 publications

Phospholipids in Milk Fat: Composition, Biological and Technological Significance, and Analytical Strategies

Phospholipids in Milk Fat: Composition, Biological and Technological Significance, and Analytical Strategies

Effects of Dairy Protein and Fat on the Metabolic Syndrome and Type 2 Diabetes

Quantification of age‐related changes of α‐tocopherol in lysosomal membranes in murine tissues and human fibroblasts

Contact Info

Product

Resources

About