Milk Fat Globule Membrane Components–A Proteomic Approach

Cavaletto, Maria; Giuffrida, Maria Gabriella; Conti, Amedeo

doi:10.1007/978-0-387-74087-4_4

Cited by 105 publications

(75 citation statements)

References 28 publications

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“…The origin of the MFGM and the mechanism of cellular milk fat secretion continue to be areas of intense investigation. Proteomic approaches have identified many of the associated proteins (Reinhardt and Lippolis, 2006;Cavaletto et al, 2008), and knock-out mouse models have demonstrated the essential role for some of these proteins in milk fat secretion including butyrophilin (Ogg et al, 2004) and xanthine oxidoreductase (McManaman et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Recent advances in the regulation of milk fat synthesis

Harvatine

Boisclair

Bauman

2009

Animal

266

272

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In addition to its economic value, milk fat is responsible for many of milk's characteristics and can be markedly affected by diet. Diet-induced milk fat depression (MFD) was first described over a century ago and remains a common problem observed under both intensive and extensive management. The biohydrogenation theory established that MFD is caused by an inhibition of mammary synthesis of milk fat by specific fatty acids (FA) produced as intermediates in ruminal biohydrogenation. During MFD, lipogenic capacity and transcription of key lipid synthesis genes in the mammary gland are down-regulated in a coordinated manner. Our investigations have established that expressions of sterol response element-binding protein 1 (SREBP1) and SREBP-activation proteins are down-regulated during MFD. Importantly, key lipogenic enzymes are transcriptionally regulated via SREBP1. Collectively, these results provide strong evidence for SREBP1 as a central signaling pathway in the regulation of mammary FA synthesis. Spot 14 is also down-regulated during MFD, consistent with a lipogenic role for this novel nuclear protein. In addition, SREBP1c and Spot 14 knock-out mice exhibit reduced milk fat similar to the magnitude and pattern of MFD in the cow. Application of molecular biology approaches has provided the latest chapter in the regulation of milk fat synthesis and is reviewed along with a brief background in nutritional regulation of milk fat synthesis in ruminants.

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Section: Introductionmentioning

confidence: 99%

Recent advances in the regulation of milk fat synthesis

Harvatine

Boisclair

Bauman

2009

Animal

266

272

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“…The milk fat globule membrane (MFGM) is the structural membrane covering triglyceride globules that are dispersed as emulsified bodies in milk (9), and it is prepared from buttermilk by filtration and centrifugation (10). The MFGM contains unique polar lipids and membrane-specific proteins (9), and several nutritional benefits of MFGM components, such as anti-cancer and anti-cholesterolemic effects, have been reported (11).…”

mentioning

confidence: 99%

“…The MFGM contains unique polar lipids and membrane-specific proteins (9), and several nutritional benefits of MFGM components, such as anti-cancer and anti-cholesterolemic effects, have been reported (11). However, the efficacy of MFGM for physical function is not fully understood.…”

mentioning

confidence: 99%

Effects of Nutritional Supplementation with Milk Fat Globule Membrane on Physical and Muscle Function in Healthy Adults Aged 60 and Over with Semiweekly Light Exercise: A Randomized Double-Blind, Placebo-Controlled Pilot Trial

Minegishi

Ota

Soga

et al. 2016

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryThis study aimed to demonstrate the beneficial effects of nutritional supplementation with dietary milk fat globule membrane (MFGM) on physical performance and skeletal muscle function in healthy adults aged 60 and over with semiweekly light exercise. The study was designed as a randomized double-blind controlled trial. Twenty-two Japanese participants (10 men, 12 women) aged 60-73 y were assigned to one of two groups (11 [5 men, 6 women] in each). One group received MFGM tablets (1 g MFGM/d), and the other received placebo tablets daily for 10 wk. Both groups participated in a twice-weekly light exercise program. Physical function tests and surface electromyography (EMG) were conducted at the baseline and after 5 and 10 wk. Chair stand time significantly shortened in both groups after 10 wk compared with that at the baseline. The average time shortened more considerably in the MFGM group than in the placebo group, although the change was not statistically significant. Both knee extension strength and the cross-sectional area of the quadriceps muscles significantly increased from baseline in the MFGM group but not in the placebo group. Surface EMG showed that muscle fiber conduction velocity increased significantly after 10 wk from the baseline only in the MFGM group. The increase from the baseline was significantly greater in the MFGM group than in the placebo group. Daily supplementation with MFGM increased motor unit action potential conduction and improved muscle strength and physical performance in healthy Japanese adults aged 60 y and over paired with semiweekly light exercise.

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“…Considering the nutraceutical and biological significance of these proteins, currently there is an increased interest in MFGM [14]. Despite MFGM proteins making up only 1-4% of the total milk protein content, it is a protein family that shows a greater complexity than other fractions in milk [15,16]. The significance of MFGM proteins has been surveyed and examined by researchers, especially their bioactivities [17,18].…”

Section: Introductionmentioning

confidence: 99%

Buffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants

2017

J App Biol Biotech

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Colostrum is the first liquid food for the new born baby, secreted after the parturition. After few days of maturation the colostrum turns to milk. The cream or fat fraction of milk consists of fat droplets composed primarily of triacylglycerols that are secreted from the apical surface of the mammary cells surrounded by cellular thin membranes membrane, the fat globule membrane (FGM), formed by proteins which have been suggested to be cholesterolemia-lowering factors, inhibitors of cancer cell growth, vitamin binders, antioxidant, bactericidal, suppressors of multiple sclerosis. FGM was isolated from buffalo colostrum and milk, the fat content was > 2-fold higher in the former than in the latter. The colostrum fat globule membrane (CFGM) fraction also showed significantly higher protein content than milk fat globule membrane (MFGM) extract. Further, the SDS-PAGE separation of the total proteins from the CFGM and MFGM showed differential banding pattern. Antioxidative property of the two FGM extracts was deliberated based on their free radical scavenging ability and reducing power. CFGM was found to exhibit a relatively higher antioxidant and reductive capacity than the MFGM fractions. The results obtained in the present study showcases the superior beneficial health effects of the CFGM over the MFGM.

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Milk Fat Globule Membrane Components–A Proteomic Approach

Cited by 105 publications

References 28 publications

Recent advances in the regulation of milk fat synthesis

Recent advances in the regulation of milk fat synthesis

Effects of Nutritional Supplementation with Milk Fat Globule Membrane on Physical and Muscle Function in Healthy Adults Aged 60 and Over with Semiweekly Light Exercise: A Randomized Double-Blind, Placebo-Controlled Pilot Trial

Buffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants

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