Milk Exosomes from Gestational Diabetes Mellitus (GDM) and Healthy Parturient Exhibit Differential miRNAs Profiles and Distinct Regulatory Bioactivity on Hepatocyte Proliferation

Zheng, Zuoping; Mo, Jianhua; Lin, Feng; Wang, J.; Chen, Junbin; Luo, Haiyan; Li, Hui; Su, Chuhong; Gu, Xiangfu; Xiong, Fei; Zha, Longying

doi:10.1002/mnfr.202300005

Cited by 3 publications

(4 citation statements)

References 41 publications

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“…Bovine milk exosomes increased intracellular miR-146a and miR-155 levels and inhibited the expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) genes, but promoted HO-1 protein expression [121]. Consistent with the results of other studies, the MDA levels were increased by camel milk exosomes in male albino rats [71]. The mechanisms underlying these oxidative stresses involve Wnt signaling, which has been proposed as the target pathway upregulated by milk exosomes [124].…”

Section: Studies On Anti-inflammatory and Antioxidant Action Of Milk ...supporting

confidence: 86%

“…The content of individual miRNAs and other non-coding RNA in milk exosomes depends on the mother's diet [67]. Milk exosomes from mothers with type 1 diabetes have also been investigated, showing aberrant levels of miRNAs [68][69][70] as well as exhibiting distinct regulatory bioactivities on hepatocyte proliferation, with the inhibition of hepatocyte proliferation by downregulating mTOR via exosomal miR-101-3p delivery [71]. Milk exosomes from obese mothers also have altered miRNA composition, whose target genes are associated with neurological diseases and psychological disorders, indicating an impact on breastfed infants [72].…”

Section: Biological Activities Of Milk Exosomesmentioning

confidence: 99%

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Application of Milk Exosomes for Musculoskeletal Health: Talking Points in Recent Outcomes

Kim,

Lee

et al. 2023

Nutrients

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Milk is a nutrient-rich food source, and among the various milks, breast milk is a nutrient source provided by mothers to newborns in many mammals. Exosomes are nano-sized membranous extracellular vesicles that play important roles in cell-to-cell communication. Exosomes originate from endogenous synthesis and dietary sources such as milk. Discovered through electron microscopy as floating vesicles, the existence of exosomes in human milk was confirmed owing to a density between 1.10 and 1.18 g/mL in a sucrose gradient corresponding to the known density of exosomes and detection of MHC classes I and II, CD63, CD81, and CD86 on the vesicles. To date, milk exosomes have been used for treating many diseases, including cancers, and are widely proposed as promising carriers for the delivery of chemotherapeutic agents. However, few studies on milk exosomes focus on geriatric health, especially sarcopenia and osteoporosis related to bone and muscle. Therefore, the present study focused on milk exosomes and their cargoes, which are potential candidates for dietary supplements, and when combined with drugs, they can be effective in treating musculoskeletal diseases. In this review, we introduce the basic concepts, including the definition, various sources, and cargoes of milk exosomes, and exosome isolation and characterization methods. Additionally, we review recent literature on the musculoskeletal system and milk exosomes. Since inflammation and oxidative stress underly musculoskeletal disorders, studies reporting the antioxidant and anti-inflammatory properties of milk exosomes are also summarized. Finally, the therapeutic potential of milk exosomes in targeting muscle and bone health is proposed.

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Section: Studies On Anti-inflammatory and Antioxidant Action Of Milk ...supporting

confidence: 86%

Section: Biological Activities Of Milk Exosomesmentioning

confidence: 99%

Application of Milk Exosomes for Musculoskeletal Health: Talking Points in Recent Outcomes

Kim,

Lee

et al. 2023

Nutrients

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show abstract

“…Human milk exosomes from gestational diabetes mellitus (GDM) and healthy parturients showed distinct regulatory bioactivities in both HepG2 cell cultures and, in vivo, in the liver of Balb/c mice. The profile of GDM exosomes has been related to natural loading by miR-101-3p [34]. Previous studies confirmed that the target gene of miR-101-3p is mTOR.…”

Section: Bioavailability Of Mature Mirnas In the Digestive Systemmentioning

confidence: 92%

“…Many short RNAs are undescribed and, beyond as an interest as biomarkers, new regulatory processes may be The recovery of exosomes is influenced by the procedure of sampling (Figure 1C) and a systematic centrifugation step of the milk before storage has been proposed in order to remove cells and debris [33]. A minimal volume of 1.5 mL whole milk has been used to purify exosomes [19], which is a technical lock, up to higher volumes like 20 mL [34].…”

Section: Rna Content and Mature Mirna Diversitymentioning

confidence: 99%

Human Breast Milk miRNAs: Their Diversity and Potential for Preventive Strategies in Nutritional Therapy

Kaeffer

2023

IJMS

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The endogenous miRNAs of breast milk are the products of more than 1000 nonprotein-coding genes, giving rise to mature small regulatory molecules of 19–25 nucleotides. They are incorporated in macromolecular complexes, loaded on Argonaute proteins, sequestrated in exosomes and lipid complexes, or present in exfoliated cells of epithelial, endothelial, or immune origins. Their expression is dependent on the stage of lactation; however, their detection depends on progress in RNA sequencing and the reappraisal of the definition of small RNAs. Some miRNAs from plants are detected in breast milk, opening the possibility of the stimulation of immune cells from the allergy repertoire. Each miRNA harbors a seeding sequence, which targets mRNAs, gene promoters, or long noncoding RNAs. Their activities depend on their bioavailability. Efficient doses of miRNAs are estimated to be roughly 100 molecules in the cytoplasm of target cells from in vitro and in vivo experiments. Each miRNA is included in networks of stimulation/inhibition/sequestration, driving the expression of cellular phenotypes. Three types of stress applied during lactation to manipulate miRNA supply were explored using rodent offspring: a foster mother, a cafeteria diet, and early weaning. This review presents the main mature miRNAs described from current mothers’ cohorts and their bioavailability in experimental models as well as studies assessing the potential of miR-26 or miR-320 miRNA families to alter offspring phenotypes.

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Human Breastmilk miRNAs, Diversity and Potentials for Preventive Strategy in Nutritional Therapy

Kaeffer

2023

Preprint

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The endogenous miRNAs of breast milk are the products of more than 1,000 nonprotein-coding genes, giving rise to mature small regulatory molecules of 19–25 nucleotides. They are incorporated in macromolecular complexes, loaded on Argonaut proteins, sequestrated in exosomes, lipid complexes, or present in exfoliated cells of epithelial, endothelial, or immune origins. Their expression is dependent on the stage of lactation, however their detection depends on progress in RNA sequencing and the reappraisal of small RNAs definition. Some miRNAs from plants are detected in breast milk, opening the possibility of stimulation of immune cells of the allergic repertoire. Each miRNA harbors a seeding sequence, which targets mRNAs, gene promoters, or long noncoding RNAs. Their activities depend on their bioavailability. Efficient doses of miRNAs are estimated at roughly 100 molecules in the cytoplasm of target cells from in vitro and in vivo experiments. Each miRNA is included in networks of stimulation/inhibition/sequestration driving the expression of cellular phenotypes. Three types of stress applied during lactation to manipulate miRNA supply, have been explored on the rodent offspring: foster mother, cafeteria diet, early weaning. The review present the main mature miRNAs described across current mothers’ cohorts, their bioavailability in experimental models, and the studies assessing the potentials of miR-26 or miR-320 miRNA families to alter offspring phenotype.

show abstract

Milk Exosomes from Gestational Diabetes Mellitus (GDM) and Healthy Parturient Exhibit Differential miRNAs Profiles and Distinct Regulatory Bioactivity on Hepatocyte Proliferation

Cited by 3 publications

References 41 publications

Application of Milk Exosomes for Musculoskeletal Health: Talking Points in Recent Outcomes

Application of Milk Exosomes for Musculoskeletal Health: Talking Points in Recent Outcomes

Human Breast Milk miRNAs: Their Diversity and Potential for Preventive Strategies in Nutritional Therapy

Human Breastmilk miRNAs, Diversity and Potentials for Preventive Strategy in Nutritional Therapy

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