Milk cellularity and intramammary infections in primiparous and multiparous Lacaune ewes during early lactation

Takano, Paulo Vitor; Scapini, Vinícius André de Cezaro; Valentini, Talita; Girardini, Lilian Kolling; Souza, Fernando Nogueira de; Libera, Alice Maria Melville Paiva Della; Heinemann, Marcos Bryan; Chande, Carla Gasparotto; Cortéz, Adriana; Collet, Silvana Giacomini; Diniz, S.A.; Blagitz, Maiara Garcia

doi:10.1016/j.smallrumres.2018.08.015

Cited by 6 publications

(2 citation statements)

References 32 publications

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“…Comparing CMT results and estimated cellularity values indicated tight correlation. Reliability of microscopically assessment was proved by high correlation coefficient (0.75) in spite of cellularity values were derived from subjective estimation (p<0.01) and not from usual microscopic somatic cell count like in other studies (Takano et al, 2018). Mean values of cellularity show about three times difference between the CMT negative and positive cases (Table 5.).…”

Section: Cellularitymentioning

confidence: 71%

Cytological and microbiological examination of bovine milk in Prototheca-infected dairy herd

Turbók¹,

Tornyos²,

Kocsis³

et al. 2022

AAK

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In healthy milk the somatic cell count (SCC) is less than 100.000 cells per mL, although in the presence of infectious agents, especially pathogenic bacteria, SCC can be extremely high. Cytological appearance of inflammation in mammary gland depends on the origin and the time-course of the inflammatory response. According to predominant cell type there can be suppurative, pyogranulomatous, granulomatous and lymphocytic inflammation. Prototheca spp. as facultative pathogen creates chronic inflammation in mammary gland with high SCC and reduced milk production. The aim of our study was to elaborate a practical and accurate laboratory procedure to enhance the precision of milk somatic cell differentiation, and to provide quick and easy-to-perform detection for the presence of infectious algae in mastitis cases. In 95 quarter samples SCC was determined by California Mastitis Test, cellular density estimation and somatic cell differentiation were carried out by microscopically evaluation, while presence of Prothoteca spp. was investigated with the help of specific culture medium (PIM agar). According to CMT results, SCC values showed 67.3% prevalence of mastitis, from which 6.3% was clinical and 93.7% subclinical. Comparing CMT results and estimated cellularity values indicated tight correlation. In CMT negative quarters 60% of somatic cells were neutrophil granulocytes, while in CMT positive but Prototheca spp. negative cases these cells were present in 74%. Interestingly in Prototheca spp. positive samples only 60.25% of cells were neutrophil granulocytes, while macrophages and lymphocytes were present in 17% and 18.3%, respectively. Moreover, remarkable cytological pattern was notified in some samples that were positive for Prototheca spp., and statistical analysis showed tight correlation between infectious algae and the ratio of macrophages (p=0.005). In conclusion laboratory techniques applied in our study seems to be helpful supplementary methods to reveal pathological background of mastitis as well as to monitor the progression of inflammatory reactions.

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Section: Cellularitymentioning

confidence: 71%

Cytological and microbiological examination of bovine milk in Prototheca-infected dairy herd

Turbók¹,

Tornyos²,

Kocsis³

et al. 2022

AAK

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“…Lacaune and their crossbreeds were also examined in seven studies [27][28][29][30][31][32][33], which were carried out in Chapeco-SC, Rio Grande do Sul, Santa Catarina, and Minas Gerais. Coagulase-negative Staphylococci were regularly obtained, especially from subclinical udder infections.…”

Section: Brazilmentioning

confidence: 99%

Coagulase-Negative Staphylococci as an Etiologic Agent of Ovine Mastitis, with a Focus on Subclinical Forms

Lysitsas,

Spyrou,

Billinis

et al. 2023

Antibiotics

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The objective of this review is to investigate the distribution and the characteristics of coagulase-negative Staphylococci (CoNS) implicated in ovine mastitis, and especially in subclinical cases, in order to provide a global perspective of the current research data and analyze specific critical aspects of the issue. PRISMA guidelines were implemented in the search of the last 20 years of the related literature in two databases. In total, 139 studies were included in this review. Relevant data were tracked down, assembled, and compared. Regarding the geographical distribution, most studies originated from Europe (68), followed by South America (33). Lacaune was the most examined breed, while S. epidermidis was the predominantly identified species, representing approximately 39% of the obtained isolates. Antibiotic resistance in the relevant bacteria was documented mostly for Penicillin (32.8%) and Amoxicillin (32.1%), while biofilm- and toxin-associated genes were encountered in variable rates because significant inequalities were observed between different articles. Significantly higher rates of antimicrobial resistance were detected in Asia and South America compared to Europe. Finally, the diagnostic procedures carried out in the respective studies were evaluated. Conventional culture and biochemical tests were mostly performed for simple strain identification; therefore, further molecular investigation of isolates should be pursued in future studies, as this will provide important data regarding specific aspects of the implication of CoNS in ovine mastitis.

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Blood polymorphonuclear leukocyte responses against Staphylococcus aureus in primiparous and pluriparous Lacaune and Santa Inês ewes

Marques

Moura

Souza

et al. 2021

Small Ruminant Research

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Milk cellularity and intramammary infections in primiparous and multiparous Lacaune ewes during early lactation

Cited by 6 publications

References 32 publications

Cytological and microbiological examination of bovine milk in Prototheca-infected dairy herd

Cytological and microbiological examination of bovine milk in Prototheca-infected dairy herd

Coagulase-Negative Staphylococci as an Etiologic Agent of Ovine Mastitis, with a Focus on Subclinical Forms

Blood polymorphonuclear leukocyte responses against Staphylococcus aureus in primiparous and pluriparous Lacaune and Santa Inês ewes

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