Milk authenticity by ion-trap proteomics following multi-enzyme digestion

Nardiello, Donatella; Natale, Anna; Palermo, Carmen; Quinto, Maurizio; Centonze, Diego

doi:10.1016/j.foodchem.2017.10.052

Cited by 34 publications

(17 citation statements)

References 30 publications

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“…Nontargeted approaches are more relevant for primary method development [98,99]. Furthermore, nontargeted proteomics approaches are suitable for the identification of biological identities and have been used for the authentication of different animal species [100], milk and dairy products [101], fish [102,103], various plant foods [104,105], as well as for the detection of pathogens [106] and production methods [107,108].…”

Section: Proteomics-based Methodsmentioning

confidence: 99%

Food authentication in real life: How to link nontargeted approaches with routine analytics?

Creydt¹,

Fischer²

2020

Electrophoresis

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In times of increasing globalization and the resulting complexity of trade flows, securing food quality is an increasing challenge. The development of analytical methods for checking the integrity and, thus, the safety of food is one of the central questions for actors from science, politics, and industry. Targeted methods, for the detection of a few selected analytes, still play the most important role in routine analysis. In the past 5 years, nontargeted methods that do not aim at individual analytes but on analyte profiles that are as comprehensive as possible have increasingly come into focus. Instead of investigating individual chemical structures, data patterns are collected, evaluated and, depending on the problem, fed into databases that can be used for further nontargeted approaches. Alternatively, individual markers can be extracted and transferred to targeted methods. Such an approach requires (i) the availability of authentic reference material, (ii) the corresponding highresolution laboratory infrastructure, and (iii) extensive expertise in processing and storing very large amounts of data. Probably due to the requirements mentioned above, only a few methods have really established themselves in routine analysis. This review article focuses on the establishment of nontargeted methods in routine laboratories. Challenges are summarized and possible solutions are presented.

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Section: Proteomics-based Methodsmentioning

confidence: 99%

Food authentication in real life: How to link nontargeted approaches with routine analytics?

Creydt¹,

Fischer²

2020

Electrophoresis

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“…Unintentional cross-contamination with milks from different ruminants may in particular arise during the processing of dairy products. Various methods including biochemical, immunochemical and molecular biology techniques have been developed to discriminate between milk proteins from different ruminants (27)(28)(29)(30). These methods are applied mostly for the detection of milk adulteration, i.e., the fraudulent incorporation of CM in GSM for economic reasons.…”

Section: Discussionmentioning

confidence: 99%

Allergen Risk Assessment for Specific Allergy to Small Ruminant's Milk: Development of Sensitive Immunoassays to Detect Goat's and Sheep's Milk Contaminations in Dairy Food Matrices

et al. 2021

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Despite a high level of sequence identity between cow's, goat's, and sheep's milk (CM, GM, and SM, respectively) proteins, some patients tolerant to CM are allergic to GM and SM. In most cases, this specificity is due to the presence of IgE antibodies that bind only to caprine and ovine caseins. The patients may then develop severe allergic reactions after ingestion of CM products contaminated with low amounts of GM or SM. We thus aimed to develop an assay able to detect traces of caprine/ovine β-caseins in different food matrices, irrespective of the presence of the bovine homolog. We produced monoclonal antibodies (mAbs) specific to caprine caseins in mice tolerized to the bovine whole casein then sensitized to the caprine whole casein. In order to develop a two-site immunometric assay, we selected mAbs that could discriminate the caprine β-casein from its bovine homolog. Characteristics and performances of two tests were determined with various dairy products. Results were analyzed in relation with the IgE-immunoreactivity of the food matrices, thanks to sera from CM, GM/SM allergic patients. Our two-site immunometric assays demonstrated a high sensitivity with a detection limit of 1.6–3.2 ng/mL of caprine and ovine β-caseins. The tests were able to detect contaminations of GM in CM at the ppm level. Heat-treatment, ripening and coagulation processes, usually applied to dairy products that exhibit a very high IgE-immunoreactivity, did not impair the test sensitivity. These quantitative assays could then be useful for the risk assessment of food products potentially contaminated with GM and SM in order to prevent adverse reactions in patients specifically allergic to these milks.

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“…Moreover, milk proteins constitute the major target for MS-based approaches to assess milk traceability and authenticity [77][78][79][80]. In this regard, a recent work published by Nardiello et al shows potentials of a proteomic workflow based on multienzyme digestion followed by nano-LC-ESI-IT-MS/MS analysis for milk authenticity [81].…”

Section: Analytical Methods For Food Authenticity and Traceabilitymentioning

confidence: 99%

Overview on Untargeted Methods to Combat Food Frauds: A Focus on Fishery Products

Fiorino

Garino

Arlorio

et al. 2018

Journal of Food Quality

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Authenticity and traceability of food products are of primary importance at all levels of the production process, from raw materials to finished products. Authentication is also a key aspect for accurate labeling of food, which is required to help consumers in selecting appropriate types of food products. With the aim of guaranteeing the authenticity of foods, various methodological approaches have been devised over the past years, mainly based on either targeted or untargeted analyses. In this review, a brief overview of current analytical methods tailored to authenticity studies, with special regard to fishery products, is provided. Focus is placed on untargeted methods that are attracting the interest of the analytical community thanks to their rapidity and high throughput; such methods enable a fast collection of "fingerprinting signals" referred to each authentic food, subsequently stored into large database for the construction of specific information repositories. In the present case, methods capable of detecting fish adulteration/substitution and involving sensory, physicochemical, DNA-based, chromatographic, and spectroscopic measurements, combined with chemometric tools, are illustrated and commented on.

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Milk authenticity by ion-trap proteomics following multi-enzyme digestion

Cited by 34 publications

References 30 publications

Food authentication in real life: How to link nontargeted approaches with routine analytics?

Food authentication in real life: How to link nontargeted approaches with routine analytics?

Allergen Risk Assessment for Specific Allergy to Small Ruminant's Milk: Development of Sensitive Immunoassays to Detect Goat's and Sheep's Milk Contaminations in Dairy Food Matrices

Overview on Untargeted Methods to Combat Food Frauds: A Focus on Fishery Products

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