Milk and serum proteomes in subclinical and clinical mastitis in Simmental cows

Turk, Romana; Rosić, N; Kuleš, Josipa; Horvatić, Anita; Gelemanović, Andrea; Galen, Asier; Ljubić, Blanka Beer; Benić, Miroslav; Stevanović, Vladimir; Mrljak, Vladimir; Chadwick, Christopher C.; Eckersall, P.D.

doi:10.1016/j.jprot.2021.104277

Cited by 32 publications

(21 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fifty natural infected CM Chinese Holstein cows, identified and confirmed by a veterinary surgeon according to the criteria of Turk 11 , with Somatic cell count (an increased SCC results from an inflammatory process due to the presence of an intramammary infection. The SCC of milk sample is 500,000 cells/ml or more, which usually indicates breast infection udder infection.)…”

Section: Methodsmentioning

confidence: 99%

“…Mastitis is the inflammation of the mammary gland, typically arising due to intramammary infections, broadly classified into subclinical (SCM) and clinical mastitis (CM) based on the severity of infection 10 . CM displays symptoms such as fever, depression, and anorexia and is usually associated with visible local and systemic signs of inflammation, marked by changes in milk, such as the appearance of clots, flakes, or watery texture 11 , 12 . CM is mainly caused by infection with pathogenic microorganisms such as Escherichia coli , Streptococcus agalactiae , and Staphylococcus aureus .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of anemoside B4 on milk whey in clinical mastitis-affected cows elucidated using tandem mass tag (TMT)-based quantitative proteomics

Shen

Zhang

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Intramuscular injection of anemoside B4 (AB4) has a superior therapeutic effect on clinical mastitis in lactating cows. Here, we explored AB4’s effect on milk whey in clinical mastitis-affected cows using proteomics. Among fifty clinical mastitis cows received AB4 administration (0.05 ml/kg/day, for 7 days), twelve healed cows were selected and marked as group T. Twelve clinically heathy cows received the same dose of saline for 7 days, marked as group C. Collected milk whey of group T before and after AB4 administration marked as T1 and T2, respectively. The milk whey of group C after saline injection marked as C1. Milk whey protein changes were detected using tandem mass tag-based quantitative proteomic. We identified 872 quantifiable proteins in the samples. Among them, 511 proteins between T1 and C1, and 361 proteins between T2 and T1 were significantly altered. T1 than C1 had significantly more proteins associated with inflammatory damage and trans-endothelial migration of leukocytes, whereas these proteins were reduced in T2 treated with AB4. Compared with C, proteins associated with fibrin clot degradation and complement system activation were downregulated in T1 but upregulated in T2. In summary, AB4 can exert its therapeutic effect on clinical mastitis in cows mainly by reducing inflammatory damage, activating the complement system, inhibiting trans-endothelial migration of leukocytes, and promoting degradation of milk fibrin clots.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of anemoside B4 on milk whey in clinical mastitis-affected cows elucidated using tandem mass tag (TMT)-based quantitative proteomics

Shen

Zhang

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Serum albumin and immunoglobulins are the chief proteins that are implicated in udder defense mechanisms (Tsenkova et al, 2001). Increased proteins in the blood of cows may be an indication of immune system activation succeeding mammary gland infection (Turk et al, 2021).…”

Section: )mentioning

confidence: 99%

Evaluation of oxidative stress, compositional and biochemical changes in milk and serum of cows with subclinical mastitis

Yehia

Ramadan

Saad

et al. 2022

Preprint

View full text Add to dashboard Cite

Twenty-five apparent healthy multiparous Holstein cows were examined for subclinical mastitis (SCM). The objectives of the study were to investigate the consequences of SCM on milk composition & blood biochemistry, oxidative stress biomarkers, and the correlation between different milk and serum parameters in SCM-affected cows. The cows were grouped as 16 subclinical infected and 9 healthy according to MSCC and California Mastitis Test (CMT) scores. The blood and milk samples were gathered from each animal and evaluated for compositional, biochemical, and oxidative stress markers alterations. SCM-affected milk samples showed a significant decrease in fat %, protein %, lactose %, TS %, SNF %, and total antioxidant capacity (TAC) and a significant increase in ash %, catalase, and malondialdehyde (MDA) compared with SCM-free milk samples. Milk somatic cell count showed a positive correlation with MDA and catalase. Catalase correlated positively with MDA and TAC. Milk fat showed a positive correlation with lactose, total protein, and albumin and a negative correlation with MSCC, MDA, TAC, and catalase. Significant increase in catalase and total protein associated with a significant decrease in Ca level was recorded in SCM blood samples compared with healthy counterparts. The total protein showed a positive relation with somatic cell count and albumin. Albumin correlated positively with TAC & catalase and negatively with Mg. The results indicate that a significant correlation between different parameters in blood and milk can be useful as a proper clinical indicator for the early detection of SCM in cows.

show abstract

“…The relative abundance of these proteins is not constant, which may be attributed to several factors, such as lactation stage, health status, feeding, and genotype [3,4]. The health status of cows is the best studied parameter, mainly focusing on variation in the milk proteome due to mastitis [5]. The milk serum proteome shown to change with progressing lactation stage, especially from colostrum to early lactation [4,6].…”

Section: Introductionmentioning

confidence: 99%

First Insight into the Variation of the Milk Serum Proteome within and between Individual Cows

Zhang

Boeren

Heck

et al. 2022

Dairy

View full text Add to dashboard Cite

Milk contains all nutrients needed for development of calves. One important group of components responsible for this are the milk proteins. Variation due to feed or animal health, has been studied for the most abundant milk proteins. The aim of this study was to determine the variation between and within cows for their milk serum proteome. Sample Set 1 was collected from Holstein Friesian (HF) cows between November 2011 and March 2012 and prepared using filter aided sample preparation (FASP) followed by LC-MS/MS for protein identification and quantification. The results showed that the milk serum proteome was very constant in mid lactation (four cows at five time points, p > 0.05) between 3 and 6 months in lactation. Sample Set 2 was collected from HF cows in Dec 2012 and analyzed using FASP and dimethyl labeling followed by LC-MS/MS. Significant variation in the milk serum proteome (p < 0.05) between 17 individual cows was found in Sample Set 2. The most variable proteins were immune-related proteins, which may reflect the health status of the individual cow. On the other hand, proteins related to nutrient synthesis and transport were relatively constant, indicating the importance of milk in providing a stable supply of nutrients to the neonate. In conclusion, the milk serum proteome was stable over mid lactation, but differed significantly between individuals, especially in immune-related proteins.

show abstract

Milk and serum proteomes in subclinical and clinical mastitis in Simmental cows

Cited by 32 publications

References 37 publications

Effect of anemoside B4 on milk whey in clinical mastitis-affected cows elucidated using tandem mass tag (TMT)-based quantitative proteomics

Effect of anemoside B4 on milk whey in clinical mastitis-affected cows elucidated using tandem mass tag (TMT)-based quantitative proteomics

Evaluation of oxidative stress, compositional and biochemical changes in milk and serum of cows with subclinical mastitis

First Insight into the Variation of the Milk Serum Proteome within and between Individual Cows

Contact Info

Product

Resources

About