Milk and kefir maintain aspects of health during doxorubicin treatment in rats

Abstract

Doxorubicin (DOX), a powerful anthracycline antibiotic commonly used to treat a wide variety of cancers, is associated with the production of reactive oxygen species that cause oxidative damage, resulting in cardiac dysfunction. Components of dairy may have protective effects against DOX-induced cardiac damage. Kefir is a naturally fermented milk product containing antioxidants, probiotic bacteria, and yeast in addition to the protective components of dairy. We explored the effects of dietary milk and kefir on DOX-induced cardiotoxicity in rats. We used singly housed, 10-wk-old male Sprague Dawley rats assigned to 1 of 3 isocaloric diets, control (CON n = 24), milk (MLK, n = 24), or kefir (KEF, n = 24), with equivalent macronutrient profiles. After a 9-wk dietary intervention, all animals were given either a bolus injection (15 mg/kg) of DOX (CON-DOX n = 12; MLK-DOX n = 12, KEF-DOX n = 12) or saline (CON-SAL n = 12; MLK-SAL n = 12; KEF-SAL n = 12). Body weight, grip strength, echocardiographic evaluation of cardiac geometry, and cardiac function were evaluated using echocardiography at 5 d postinjection and data were analyzed using ANOVA. Survival at d 5 post-DOX injection was 92 and 100% in KEF-DOX and MLK-DOX, respectively, and 75% in CON-DOX. By the last week of the dietary intervention, and just before injection with saline or DOX, CON weighed significantly (14%) more than the MLK and KEF. The DOX treatment resulted in significant reductions in body weight; however, we found no diet × drug interactions. The DOX treatment reduced peak grip strength compared with SAL; when compared with pre-injection measures, MLK-DOX rats did not experience a significant reduction in peak grip strength compared with CON-DOX and SAL-DOX rats. Heart mass in MLK and KEF was significantly higher when compared with CON. In summary, 9 wk of milk or kefir ingestion resulted in lower body size and higher heart mass after DOX treatment. Additionally, MLK preserved peak grip strength after DOX treatment, whereas KEF or CON did not. We observed no consistent protective effects with respect to heart dimensions and function. These findings suggest that long-term milk or kefir ingestion may be helpful in optimizing health before and during doxorubicin treatment.

Role of gut microbiota in doxorubicin-induced cardiotoxicity: from pathogenesis to related interventions

“…Gut microbiota is closely linked to daily diets, and even a short-term adjustment in the diet, as short as 5 days, is sufficient to alter the composition of gut microbiota and induce corresponding changes for adaptation [138]. Stewart et al discovered that long-term ingestion of milk or kefir (a milk containing lactic acid bacteria) could be beneficial for rats treated with doxorubicin [139]. Additionally, it has been demonstrated that the administration of phenylalanine-butyramide (a novel synthetic derivative of butyric acid) can have a positive effect on DIC [18].…”

Effect of camel milk and pistachio-based product on cancer-induced sarcopenia in Sprague Dawley rats

“…The weight of each rat was measured on a weekly basis using a weight scale following the method described by Stewart et al (2019).…”

Potensi Whey Kefir Susu Kambing Sebagai Anti-Obesitas Melalui Penghambatan Sintesis Lipid dan Aktivitas Phosphoenolpyruvate Carboxykinase (PEPCK) pada Sel Model Adiposit 3T3-L1

“…Secara invitro dan in vivo, peningkatan berat badan diikuti dengan peningkatan kolesterol [72] Bakteri dan khamir sebagai komponen kefir menghasilkan bile salt hydrolase, enzim katalase ini mampu dekonjugasi asam empedu dan meningkatkan ekskresi kolesterol dalam fases, sehingga membantu penurunan TC [82]. Beberapa komponen bioaktif kefir menurunkan TC dan LDL manusia [74], kefir dapat mengikat lemak dan mengekresikan lemak melalui fases, juga mengakibatkan penurunan berat badan [83]. Kefir kaya asam amino [30], yang secara signifikan mengurangi ekspresi SREBP1c, PPARγ, and FAS, tetapi tidak mengurangi ACC adiposit 3T3-L1 matur.…”