Mild hypothermia reduces the inflammatory response and hepatic ischemia/reperfusion injury in rats

Behrends, Matthias; Hirose, Ryutaro; Serkova, Natalie J.; Coatney, John L.; Bedolli, Melanie; Yardi, Jason; Park, Yeon Ho; Niemann, Claus U.

doi:10.1111/j.1478-3231.2006.01292.x

Cited by 31 publications

(42 citation statements)

References 32 publications

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“…However the temperature had not interfered with the TBARS results. The significantly lesser value of TBARS when I/R was not present is an evidence that free radicals production happened on the presence of I/R, because TBARS is a very sensible marker of lipid peroxidation of the cell membrane 3 . The most interesting outcome, however, came from the assessment of CAT, which is a quite important enzyme.…”

Section: Discussionmentioning

confidence: 99%

“…To systemic assessment, f2-isoprostanes (F2IP) from plasma can be useful to observe lipid peroxidation. Nitrites (NO 2 ) and nitrates (NO 3 water, but without food. After 240 minutes of reperfusion, the rat was moved from the cage to the surgical table.…”

Section: Methodsmentioning

confidence: 99%

“…It is known that hypothermia is associated to protection against I/R injury 1 , however there is not any experimental systematic study in vivo that demonstrate what range of temperature protects kidney, although this information has already been available for the liver 2,3 . That gap of information is important because is relevant know how much cooling is necessary to achieve renal protection without causing hypothermic damage 8,10 .…”

Section: Introductionmentioning

confidence: 99%

“…Also, it is important to mention that slight hypothermia has a lesser interference in the systemic temperature 4 . Although the demonstration that mild hypothermia is sufficient to offer liver protection against the deleterious actions of I/R, there are no studies concerning the kidney 2,3 .…”

Section: Introductionmentioning

confidence: 99%

“…Even though the preconized temperature to obtain preservation is around 4ºC, there are evidences that much higher temperatures, 26ºC or even 34ºC, are enough to achieve liver protection without necessity of deeper hypothermia 2,3 . It is known that is easier to obtain mild or moderate cooling, and that deep hypothermia is more associated with thermal injury of tissue.…”

Section: Introductionmentioning

confidence: 99%

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Oxidative stress evaluation of ischemia and reperfusion in kidneys under various degrees of hypothermia in rats

Santos

Koff

Filho³

et al. 2013

Acta Cir. Bras.

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PURPOSE:To design an animal model of ischemia-reperfusion (I/R) in kidneys and evaluate the role that predetermined ranges of local hypothermia plays on markers of stress-oxydative as well as on histologic sections. METHODS:Twenty eight male rats Wistar, under general anesthesia, undergone right nephrectomy (G0, control group) followed by left kidney ischemia during 40 min. Four temperatures groups were designed, with seven animals randomized for each group: normothermic (G1, ±37 o C), mild hypothermia (G2, 26 o C), moderate hypothermia (G3, 15 o C) and deep hypothermia (G4, 4 o C). Left kidney temperature was assessed with an intraparenchymal probe. Left nephrectomy was performed after 240 min of reperfusion. After I/R a blood sample was obtained for f2-IP. Half of each kidney was sent to pathological evaluation and half to analyze CAT, SOD, TBARS, NO 3 , NO 2 . RESULTS:Histopathology showed that all kidneys under I/R were significantly more injured than the G0 (p<0.001). TBARS had increased levels in all I/R groups compared with the G0 (p<0.001). CAT had a significant difference (p<0.03) between G1 and G4. Finally, no difference was found on SOD, NO 3 , NO 2 nor on f2-IP. CONCLUSION:This model of I/R was efficient to produce oxidative-stress in the kidney, showing that 4ºC offered significant decrease in free radicals production, although tissue protection was not observed.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Oxidative stress evaluation of ischemia and reperfusion in kidneys under various degrees of hypothermia in rats

Santos

Koff

Filho³

et al. 2013

Acta Cir. Bras.

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Anti‐inflammatory signaling during ex vivo liver perfusion improves the preservation of pig liver grafts before transplantation

et al. 2016

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Normothermic ex vivo liver perfusion (NEVLP) improves graft preservation by avoiding cold ischemia injury. We investigated whether the protective effects of NEVLP can be further improved by applying strategies targeted on reducing the activation of proinflammatory cytokines during perfusion. Livers retrieved under heart-beating conditions were perfused for 4 hours. Following the preservation period, a pig liver transplantation was performed. In group 1 (n = 5), anti-inflammatory strategies (alprostadil, n-acetylcysteine, carbon monoxide, sevoflurane, and subnormothermic temperature [33°C]) were applied. This was compared with a perfused control group (group 2) where livers (n = 5) were perfused at 37°C without anti-inflammatory agents, similar to the setup used in current European clinical trials, and to a control group preserved with static cold storage (group 3). During 3-day follow-up, markers of reperfusion injury, bile duct injury, and liver function were examined. Aspartate aminotransferase (AST) levels during perfusion were significantly lower in the study versus control group at 1 hour (52 ± 6 versus 162 ± 86 U/L; P = 0.01), 2 hours (43 ± 5 versus 191 ± 111 U/L; P = 0.008), and 3 hours (24 ± 16 versus 218 ± 121 U/L; P = 0.009). During perfusion, group 1 versus group 2 had reduced interleukin (IL) 6, tumor necrosis factor α, and galactosidase levels and increased IL10 levels. After transplantation, group 1 had lower AST peak levels compared with group 2 and group 3 (1400 ± 653 versus 2097 ± 1071 versus 1747 ± 842 U/L; P = 0.47) without reaching significance. Bilirubin levels were significantly lower in group 1 versus group 2 at day 1 (3.6 ± 1.5 versus 6.60 ± 1.5 μmol/L; P = 0.02) and 3 (2 ± 1.1 versus 9.7 ± 7.6 μmol/L; P = 0.01). A trend toward decreased hyaluronic acid, as a marker of improved endothelial cell function, was observed at 1, 3, and 5 hours after reperfusion in group 1 versus group 2. Only 1 early death occurred in each group (80% survival). In conclusion, addition of anti-inflammatory strategies further improves warm perfused preservation. Liver Transplantation 22 1573-1583 2016 AASLD.

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Mild hypothermia ameliorates hepatic ischemia reperfusion injury by inducing RBM3 expression

et al. 2022

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Mild hypothermia reduces the inflammatory response and hepatic ischemia/reperfusion injury in rats

Abstract: Mild hypothermia of 34 degrees C is sufficient to reduce I/R injury by inhibiting the inflammatory response. Further spontaneous cooling to 31 degrees C did not demonstrate any additional protective effect.

Cited by 31 publications

References 32 publications

Oxidative stress evaluation of ischemia and reperfusion in kidneys under various degrees of hypothermia in rats

Oxidative stress evaluation of ischemia and reperfusion in kidneys under various degrees of hypothermia in rats

Anti‐inflammatory signaling during ex vivo liver perfusion improves the preservation of pig liver grafts before transplantation

Mild hypothermia ameliorates hepatic ischemia reperfusion injury by inducing RBM3 expression

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