Milchuntersuchung

Palmer, L. S.; Weaver,; Matthews, Anne; Comel,; Quam,; Hellwig, Michael; Hilditch, T. P.; Jones, E. E.; Dam, Hubertus J. J. van; Holwerda,; Richarson,; Pesch, K. L.; Simmert, U.; Düring, A.; Holborow, A. G.

doi:10.1007/bf01362069

“…3b). Discovered by Weselsky (Weselsky 1871) in the nineteenth century and used to assess metabolic activity since the late 1920s (for the evaluation of microbial contamination in milk) (Palmer et al 1930), this redox indicator has been applied to study the growth/viability of different organisms, ranging from bacteria to mammalian cells (O'Brien et al 2000).…”

Section: Resazurin-based Assaymentioning

confidence: 99%

Toxicity Assessment of Nanomaterials

Tasso

¹

,

Huvelle

²

,

Bessone

³

et al. 2020

Nanomedicine and Nanotoxicology

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In the last decades, nanoscience had a spectacular evolution providing new, versatile engineered nanomaterials and nanotools with a plethora of applications in very diverse fields ranging from energy storage to medicine. Among the palette of nanomaterials, magnetic nanoparticles (in particular iron oxide-based) present unique physicochemical properties that are actively being exploited in the biomedical field. Currently, they are used for induced magnetic hyperthermia cancer treatments, as contrast agents for magnetic resonance imaging, as cell tracking elements, and for drug delivery modalities. In parallel to the growth of nanoscience and the everincreasing applications of nanomaterials, concerns regarding the safety and toxicity of nanoparticles have arisen, both during and post-administration. In this chapter, we review key concepts related to nanotoxicology and to the fate of nanomaterials in the human body. A detailed description about the most accepted and practiced in vitro and in vivo methods used to evaluate the toxicity of nanomaterials is provided, with emphasis in magnetic nanomaterials for nanomedicine applications.

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“…3b). Discovered by Weselsky (Weselsky 1871) in the nineteenth century and used to assess metabolic activity since the late 1920s (for the evaluation of microbial contamination in milk) (Palmer et al 1930), this redox indicator has been applied to study the growth/viability of different organisms, ranging from bacteria to mammalian cells (O'Brien et al 2000).…”

Section: Resazurin-based Assaymentioning

confidence: 99%

Magnetic Nanoheterostructures

2020

Nanomedicine and Nanotoxicology

4

0

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In the last decades, nanoscience had a spectacular evolution providing new, versatile engineered nanomaterials and nanotools with a plethora of applications in very diverse fields ranging from energy storage to medicine. Among the palette of nanomaterials, magnetic nanoparticles (in particular iron oxide-based) present unique physicochemical properties that are actively being exploited in the biomedical field. Currently, they are used for induced magnetic hyperthermia cancer treatments, as contrast agents for magnetic resonance imaging, as cell tracking elements, and for drug delivery modalities. In parallel to the growth of nanoscience and the everincreasing applications of nanomaterials, concerns regarding the safety and toxicity of nanoparticles have arisen, both during and post-administration. In this chapter, we review key concepts related to nanotoxicology and to the fate of nanomaterials in the human body. A detailed description about the most accepted and practiced in vitro and in vivo methods used to evaluate the toxicity of nanomaterials is provided, with emphasis in magnetic nanomaterials for nanomedicine applications.

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Basic Colorimetric Proliferation Assays: MTT, WST, and Resazurin

Präbst¹,

Engelhardt²,

Ringgeler³

et al. 2017

Methods in Molecular Biology

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This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures. The underlying principle of these assays is the measurement of a biochemical marker to evaluate the cell's metabolic activity. The formation of the omnipresent reducing agents NADH and NADPH is used as a marker for metabolic activity in the following assays. Using NADH and NADPH as electron sources, specific dyes are biochemically reduced which results in a color change that can be determined with basic photometrical methods. The assays selected for this chapter include MTT, WST, and resazurin. They are applicable for adherent or suspended cell lines, easy to perform, and comparably economical. Detailed protocols and notes for easier handling and avoiding pitfalls are enclosed to each assay.

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Milchuntersuchung

Cited by 4 publications

References 0 publications

Toxicity Assessment of Nanomaterials

Toxicity Assessment of Nanomaterials

Magnetic Nanoheterostructures

Basic Colorimetric Proliferation Assays: MTT, WST, and Resazurin

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