Mikrobieller Abbau von Glucosinolaten im Rapsextraktionsschrot

Huber, John D.; Kranz, G.; Kreibich, G.; Beining, K.; Krüger, Michael; Weißbach, Friedrich

doi:10.1002/food.19830270319

Cited by 12 publications

(11 citation statements)

References 12 publications

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“…Most of microorganisms were isolates of F. oxysporum, and Bacillus spp. The sinigrin degradation effects of Bacillus cereus and F. oxysporum were noticed in early reports (Huber et al 1983;Smits et al 1993). Degradation of sinigrin by Streptomyces spp.…”

Section: Discussionmentioning

confidence: 94%

“…Myrosinases are enzymes responsible for the hydrolysis of glucosinolates (Chew 1988). Although myrosinases have mainly been isolated from a number of plant sources (Fenwick et al 1983;Rask et al 2000), there are some reports concerning myrosinase activity from microorganisms such as Escherichia coli (Migula) Castellani & Chalmers (Oginsky et al 1965), Enterobacter cloacae (Jordan) Hormaeche & Edwards (Tani et al 1974), Bacillus cereus Frankland & Frankland (Huber et al 1983), Aspergillus niger Tiegh. (Ohtsuru and Hata 1973), A. sydowi (Bainier & Sartory) Thom & Church (Reese et al 1958), A. ficuum (Reichardt) Hennings (Nair and Duvnjak 1990), A. clavatus Desm., Fusarium oxysporum Schlechtend.:Fr.…”

Section: Introductionmentioning

confidence: 99%

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Control, byBrassicaseed pomace combined withPseudomonas boreopolis, of damping-off of watermelon caused byPythiumsp.

Chung¹,

Huang

et al. 2003

Canadian Journal of Plant Pathology

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A total of 57 isolates of fungi, bacteria, and actinomycetes were screened for their ability to degrade the glucosinolate sinigrin. Pseudomonas boreopolis was the most effective among 26 isolates having the capacity to degrade sinigrin. Treatment with ground Brassica seed pomace and Pseudomonas boreopolis resulted in the release of volatile compounds. Gas-chromatography analyses allowed to identify allyl isothiocyanate and 3-butenyl isothiocyanate as the major volatile compounds released from enzymatic hydrolysis of glucosinolates by Pseudomonas boreopolis. These compounds had strong fungicidal activity against Rhizoctonia solani AG-4, Sclerotium rolfsii, Sclerotinia sclerotiorum, Pythium aphanidermatum, and Phytophthora capsici as revealed by reduced mycelial growth. They were also responsible for reducing the survival of zoospores and oospores of P. aphanidermatum. A large-scale greenhouse trial showed that amendment of growth media (Bas Van Buuren medium No. 4, 7mL; soil, 3 g) with 1% (mass fraction) of the ground Brassica seed pomace and Pseudomonas boreopolis (1 × 10 8 to 1.8 × 10 8 cfu/mL) was effective in controlling damping-off of watermelon caused by Pythium sp., resulting in increased production of healthy, marketable seedlings.Résumé : Le potentiel de dégradation de la sinigrine, un glucosinolate, fut testé chez 57 isolats de champignons, bactéries ou actinomycètes. Parmi 26 isolats ayant manifesté une capacité à dégrader la sinigrine, le Pseudomonas boreopolis s'est révélé le plus efficace. Lors de l'addition du Pseudomonas boreopolis au marc de graines de Brassica broyées, des composés volatils furent libérés. Les analyses par chromatographie en phase gazeuse ont montré que l'allylisothiocyanate et le 3-buténylisothiocyanate étaient les principales substances volatiles libérées par l'hydrolyse enzymatique des glucosinolates par le Pseudomonas boreopolis. Ces substances ont affiché une intense activité fongicide contre le Rhizoctonia solani AG-4, le Sclerotium rolfsii, le Sclerotinia sclerotiorum, le Pythium aphanidermatum et le Phytophthora capsici, tel que révélé par la croissance mycélienne réduite. Ces substances ont aussi diminué la survie de zoospores et d'oospores de P. aphanidermatum. Un essai en serre à grande échelle a montré que l'ajout au milieu de croissance (milieu Bas Van Buuren n o 4, 7mL; sol, 3 g) de 1% (fraction massique) de marc de graines de Brassica broyées et du Pseudomonas boreopolis (1 × 10 8 à 1,8 × 10 8 cfu/mL) était efficace à contrer la fonte des semis du melon d'eau causé par le Pythium sp., donnant ainsi une plus grande production de semis commercialisables sains. Mots clés : marc de graines de Brassica, allylisothiocyanate, 3-buténylisothiocyanate, fonte des semis, melon d'eau, Pythium aphanidermatum, Pseudomonas boreopolis. Chung et al.: damping-off / Pythium sp. / soil amendments / biological control 294

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Control, byBrassicaseed pomace combined withPseudomonas boreopolis, of damping-off of watermelon caused byPythiumsp.

Chung¹,

Huang

et al. 2003

Canadian Journal of Plant Pathology

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“…1965), Aspergillus niger (Ohtsuru and Hata 1973), the yeast Geotrichum candidum (Staron 1975), Enterobacter cloacae (Tani et al. 1974), Bacillus cereus (Huber et al. 1983), Lactobacillus agilis R16 (Llanos Palop et al.…”

Section: Introductionmentioning

confidence: 99%

The antimicrobial effects of glucosinolates and their respective enzymatic hydrolysis products on bacteria isolated from the human intestinal tract

Aires

Mota

Saavedra

et al. 2009

Journal of Applied Microbiology

156

119

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Aims: The aim of the study was to evaluate the in vitro antibacterial activity of glucosinolates and their enzymatic hydrolysis product against bacteria isolated from the human intestinal tract. Methods and results: Using a disc diffusion bioassay, different doses of intact glucosinolates and their corresponding hydrolysis products were tested. There were clear structure–activity and concentration differences with respect to the in vitro growth inhibition effects as well as differences in the sensitivities of the individual bacteria. The most effective glucosinolate hydrolysis products were the isothiocyanates; sulforaphane and benzyl isothiocyanate were the best inhibitors of growth. Indole‐3‐carbinol had some inhibitory effects against the Gram‐positive bacteria but had no effect, even at the highest dose, against the Gram‐negative bacteria. Indole‐3‐acetonitrile had some inhibitory activity against the Gram‐negative bacteria. Glucosinolates, nitriles and amines were ineffective at all the doses used. Conclusions: Glucosinolate hydrolysis products and specifically the isothiocyanates SFN and BITC have significant antimicrobial activity against Gram‐positive and Gram‐negative bacteria, and might be useful in controlling human pathogens through the diet. Significance and Impact of the Study: This the first major in vitro study demonstrating the potential of these natural dietary chemicals as an alternative to, or in combination with, current antibiotic‐based therapies for treating infectious diseases.

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“…were not substantially toxic during the 60 min of incubation at 37°C (Table 2). There are reports in the literature which indiVolatility of toxicants cate that some microorganisms, including yeast, possess Plant myrosinase is reported to have an optimum tempera-P-thioglucosidase activity (Huber et al 1983;Reese et al ture of 55°C for its activity, but in the presence of L-ascorbate 1958). However, the slight decreases in yeast viability, noted this is reduced to about 35°C (Ohtsura and Hata 1979).…”

Section: Results and Discussion Seed Hoinogeizate Toxicitymentioning

confidence: 97%

“…Volatiles produced naturally by hydrolysis of glucosinolates in cruciferous plants have occasionally been implicated as growth inhibitors of bacteria or have been shown to have lethal activity toward microorganisms of various types (Anderson et al 1953;Fenwick et al 1983;Huber et al 1983;Virtanen 1962). It is also possible that in host plants these compounds may confer resistance against infection by Peronospora pamsiticia, the organism responsible for cabbage downy mildew, or Plasmodiophora brassicae Woron., the agent responsible for clubroot infection in the crucifers.…”

Section: Seed Toxicitymentioning

confidence: 98%

The role of myrosinase in the development of toxicity toward Nematospora in mustard seed

Holley¹,

Jones²

1985

Can. J. Bot.

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The toxicity of contaminated mustard seed toward Nematospora yeast was due to the action of endogenous myrosinase upon the glucosinolates, sinigrin from Brassicajuncea, and sinalbin from B. hirta seeds to produce toxic hydrolysis products. Allyl isothiocyanate from sinigrin was lethal at 20–35 μg/mL while p-hydroxybenzyl alcohol and p-hydroxybenzoic acid from sinalbin were toxic at >600 and >800 μg/mL, respectively. Two cultivars of rapeseed (B. campestris cv. Echo and cv. Candle) contained only trace amounts of sinigrin and sinalbin and were not toxic to the yeast. The activation of myrosinase during germination of oriental and yellow mustard and the production of toxic hydrolytic compounds may limit the spread of infection by reducing the reservoir of viable yeast in the seed. No evidence was obtained to suggest a role for these toxic products in resistance of the plant to invasion by Nematospora.

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Mikrobieller Abbau von Glucosinolaten im Rapsextraktionsschrot

Cited by 12 publications

References 12 publications

Control, byBrassicaseed pomace combined withPseudomonas boreopolis, of damping-off of watermelon caused byPythiumsp.

Control, byBrassicaseed pomace combined withPseudomonas boreopolis, of damping-off of watermelon caused byPythiumsp.

The antimicrobial effects of glucosinolates and their respective enzymatic hydrolysis products on bacteria isolated from the human intestinal tract

The role of myrosinase in the development of toxicity toward Nematospora in mustard seed

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