Today, the use of micro-computed tomography is becoming widespread in almost every field of dental research. When the national review articles on micro-computed tomography are examined, it is seen that the focus is on endodontic and surgical dental applications. As prosthodontists, our article, which was compiled in order to deepen the specific usage areas of micro-computed tomography applications and to review the studies done in this area, provides information about the use of micro-computed tomography method in prosthetic dentistry. Micro-computed tomography is a powerful in vitro research method. Micro-computed tomography was used; marginal and internal compatibility of restorations, cement spacing of restorations, adaptation of denture bases, accuracy of prosthetic measurements, effects of occlusal irregularities, volumetric changes in teeth due to dental post application, biomechanical evaluation of implant-abutment connection, and compatibility of maxillofacial prostheses.