Migratory behavior of lymphocytes with specific reactivity to alloantigens. II. Selective recruitment to lymphoid cell allografts and their draining lymph nodes.

Abstract

A dual-antigen, dual-isotope assay has been used to monitor the migratory behavior of selectively labeled antiallogeneic lymphocytes in mice challenged subcutaneously in all four foot pads with semiallogeneic spleen cells. 3H-labeled anti-C3H and 14C-labeled anti- C57BL lymphocytes of DBA/2J origin were pooled and adoptively transferred to multiple groups of previously challenged DBA/2J recipients. In some of the studies, separate groups of recipients were challenged with either CDF or BDF spleen cells in all four paws, whereas in others CDF spleen cells were used to challenge the right paws of each mouse in the group and BDF spleen cells to challenge the left paws of each mouse in the group. At intervals varying from 24 to 96 h after challenge, a subgroup of four mice from each appropriate group was sacrificed and the relative numbers of anti-C3H and anti- C57BL lymphocytes present in the challenged paws, draining lymph nodes, and other tissues of each mouse were inferred from the mean 3H/14C ratios of the respective tissues of that subgroup. The results of these studies firmly establish that specific antiallogeneic lymphocytes are selectively recruited to the paws and draining lymph nodes of mice challenged subcutaneously in the foot pads with semiallogeneic spleen cells and are deleted from their circulating blood and nondraining lymph nodes. A mechanism for antigen-induced selective recruitment and its possible functional significance in tumor immunology are discussed.

Redistribution of renal allograft-responding leukocytes during rejection. II. Kinetics and specificity.

“…This very high turnover rate is a new and unexpected finding, but is compatible with the high turnover rate of inflammatory (white) cells in other sites of immune activation, e.g., complete Freund's adjuvant-induced granulomas (24) and lymph nodes responding to allogeneic lymphocytes or tuberculin (25). Our estimate on the inbound traffic to the allograft is far higher than any one of the previous estimates (6,(9)(10)(11) based on reinfusion of in vitro or in vivo labeled spleen or thoracic duct leukocytes into the graft-carrying host. It should be emphasized that, in contrast to the previous analysis, no significant sequestration of labeled cells (over the blood background level) was observed in our study into irrelevant positions, such as the recipient liver or lung.…”

Demonstration of Accelerated and Increased Migration Inhibition Factor Release in vivo in a PPD Retest Reaction

“…This would agree with the generally held opinion that lymphocytes enter inflam matory sites nonspecifically [8,12,19,22]. However, slight preferential accumulation of antigen-specific lymphocytes ( 1.5-to 3-fold as compared to nonspecif ic lymphocytes) has been found in many inflammato ry reactions [7,13,21], and is probably responsible for the reported antigen specificity of skin retest reactions [1,14], Using the present migration inhibition assay, we understood from the cell dilution experiments that we would not have been able to detect a smaller than a 2-fold increase in numbers of antigen-specific cells. One would expect the generation of higher percen tages of antigen-specific lymphocytes at inflammato ry sites only following systemic stimulation of anti gen-specific cells [2], or when further lymphoid cell proliferation occurs at the inflammatory sites.…”

Cellular Immunity Induced by Lung Immunization of Fischer 344 Rats

“…The results of various studies have suggested that lymphocytes do home specifically to tissues that contain im munizing antigen [23,24], Further study is required to determine if these lymphoid cells were produced in the spleen and homed to the lung-associated lymph nodes because they retained small amounts of an tigen, or whether the cell division observed in the lung-associated lymph nodes by eval uating 3H-thymidine uptake in control cul tures was responsible for the 12-through 17-day response.…”