Migration of transfused granulocytes in leukopenic dogs

Abstract

Although granulocyte transfusion therapy has been shown to be effective in infected granulocytopenic animals and humans, the relative effectiveness of granulocytes (PMN) harvested by continuous flow centrifugation (CFC) or by continuous flow filtration leukapheresis (FL) remains uncertain. Studies in vitro of morphology and granulocyte functions have suggested cells collected by FL may be damaged. To compare the function in vivo of granulocytes collected by different methods, dogs were made granulocytopenic with cyclophosphamide (CYT) and then transfused with granulocytes collected by CFC or FL. The local neutrophil mobilization (LNM) through a standard skin abrasion into a chamber containing a strong chemoattractant, autologous serum, was measured. Greater LNM was found after transfusions of CFC PMN than after transfusions of the same number of FL PMN (p less than 0.0003). This difference persisted even when the dose of FL PMNs was four times greater than that of CFC mn and when the FL donor was pretreated with steroids (p less than 0.001). These results suggest that during filtration leukapheresis, granulocytes are functionally altered and that their function in vivo may be compromised.

Experimental bacterial keratitis in neutropenic guinea pigs: polymorphonuclear leukocytes in corneal host defense

“…As a means for the induction of neutropenia, whole-body X-irradiation has some advantages over the use of cytotoxic agents such as cyclophosphamide. When single doses of such drugs are used, marrow suppression is only transitory with rapid return of marrow function (1,6). Our animals remained neutropenic for more than a week.…”

“…Patients receiving such agents may be more susceptible to bacterial or fungal corneal infection (4).The effect of neutropenia on the progress of bacterial keratitis or on the inflammatory response in the cornea has not been quantitatively assessed. By using analytic methods which allowed measurement of the ingress of PMNs into experimentally infected corneas and assay of the number of viable bacteria present, we demonstrated that PMNs play a major role in the response of the cornea to bacterial infection and that neutropenia alters that inflammatory response.(This paper was presented in part at the 18th Interscience Conference on Antimicrobial Agents and Chemotherapy, Atlanta, Ga., [1][2][3][4] October 1978.) MATERIALS AND METHODS Induction of neutropenia.…”

“…(This paper was presented in part at the 18th Interscience Conference on Antimicrobial Agents and Chemotherapy, Atlanta, Ga., [1][2][3][4] October 1978.) MATERIALS AND METHODS Induction of neutropenia.…”

“…Quantitative techniques were used to determine the relative concentrations of viable bacteria and polymorphonuclear leukocytes (PMNs) in the corneas of neutropenic and non-neutropenic guinea pigs with experimental bacterial keratitis induced with three strains of Pseudomonas aeruginosa. Neutropenia was produced by whole-body X-irradiation 1 week before infection. Significantly greater numbers of bacteria were present in the corneas of neutropenic animals 48 h after infection than were present in the corneas of non-neutropenic animals.…”