Migration of muscle cells

Watt, Diana J.; Karasiński, J; Moss, Jill; England, Marjorie A.

doi:10.1038/368406a0

Cited by 61 publications

(42 citation statements)

References 13 publications

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“…mice, the highest percentages of dystrophin-positive fibers were observed only after damaging the irradiated In an article by Watt et al (40), which had particularly encouraged us to improve the migratory potential host muscles or by performing successive transplantations to stimulate regeneration (10,17). In primates, we of the transplanted myoblasts, it was reported that C2C12 injected into a muscle could form hybrid myohave also demonstrated that successful myoblast transplantation depends on appropriate cell delivery and infibers in the adjacent muscle.…”

Section: Follow-up Of Myogenic Cell Transplantationmentioning

confidence: 99%

Growth Factor Coinjection Improves the Migration Potential of Monkey Myogenic Precursors without Affecting Cell Transplantation Success

et al. 2009

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Duchenne muscular dystrophy (DMD) is an inherited disease and a main target of myogenic cell transplantation (MT). After the failure of the first clinical trials with DMD patients, the poor migration of transplanted cells has been suspected to be a major problem for a more effective clinical application of MT. Previous investigations suggested that the quantity and dispersion of myofibers containing donor cell nuclei might be improved by increasing the migration of the transplanted cells outside the injection sites. Because the coinjection of motogenic factors with human myoblasts enhanced their intramuscular migration following MT in SCID mice, the present study aimed to investigate whether this approach was appropriate to increase MT success in muscles of nonhuman primates. In vitro studies indicated that IGF-1 or bFGF increased components of proteolytic systems involved in myoblast migration. In vitro and in vivo experiments also demonstrated that coinjection of bFGF or IGF-1 was able to improve monkey myogenic cell migration and invasion. Sixty hours after MT in skeletal muscle tissue, the migration distances reached by monkey myoblasts increased by nearly twofold when one of the growth factors was coinjected with the cells. However, longterm observations in adult monkeys suggest that promigratory treatments are not intrinsically sufficient to improve the success of MT. Even if short-term observations reveal that grafted cells are not always trapped inside the injection site and in spite of the fact that both factors enhanced transplanted cell migration, myofibers including grafted cell nuclei were still restrained to the injection trajectory without notable difference in their amount or their dispersion. The incapacity of transplanted cells to fuse with undamaged myofibers, which are located outside the injection sites, is a priority problem to solve in order to improve transplantation success and reduce the number of injections required for the treatment of DMD patients.

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Section: Follow-up Of Myogenic Cell Transplantationmentioning

confidence: 99%

Growth Factor Coinjection Improves the Migration Potential of Monkey Myogenic Precursors without Affecting Cell Transplantation Success

et al. 2009

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“…Endothelial migration is deeply associated with angiogenesis, and migration of skeletal muscle precursor cells is a crucial step in skeletal muscle development and postlesional muscle regeneration. 18,40,41 Therefore, coexpression of both isoforms of HGF, which can be achieved by introducing the alternative splicing function to the expression system, may provide an effective way of inducing neovascularization and regeneration of ischemic tissues in human critical limb ischemia that may result from ischemic diabetic complications and renal failureassociated cases.…”

Section: Coexpression Of Hgf Two Isoforms Improves Critical Limb Ischmentioning

confidence: 99%

Naked DNA expressing two isoforms of hepatocyte growth factor induces collateral artery augmentation in a rabbit model of limb ischemia

et al. 2010

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Hepatocyte growth factor (HGF) has been shown to induce angiogenesis in vivo and has potential as a candidate gene for 'therapeutic angiogenesis'. In vivo, two isoforms of HGF, HGF 723 and HGF 728 , consisting of 723 and 728 amino acids, are generated through alternative splicing between exons 4 and 5, but the biological effects of their coexpression have not yet been elucidated. In this study, we generated a series of genomic-complementary DNA (cDNA) hybrids of the HGF gene by inserting various truncated intron 4 into the junction of exons 4 and 5 of HGF cDNA and analyzed the biological activities of these hybrid constructs. We showed that: (1) the hybrid called HGF-X7, which contained 1502 base pairs of intron 4, could drive a higher level of HGF expression than other hybrid constructs and cDNAs of each isoform alone; (2) the pCK vector was most efficient for the gene expression of HGF-X7; (3) coexpression of both isoforms of HGF could more efficiently induce the migration of human umbilical vein endothelial cell (HUVEC) and of the mouse myoblast cell line C 2 C 12 myoblasts than a single isoform of HGF and human vascular endothelial growth factor (VEGF) 165 at a given concentration; (4) intramuscular administration of pCK-HGF-X7 resulted in transient and localized HGF expression in the injected muscle without an increase in the HGF protein levels in other tissues including serum; and (5) intramuscular injection of pCK-HGF-X7 could more efficiently increase the number of angiographically recognizable collateral vessels, as well as improve an intra-arterial Doppler wiremeasured blood flow in the rabbit model of hindlimb ischemia when compared with the identical vector encoding VEGF 165 gene. These results showed that transfer of the genomiccDNA hybrid of the HGF gene could be used as a potential therapeutic approach to human vascular diseases.

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“…Transplantation of a single muscle fibre containing a few SC is able to generate tens of thousands of cells in a matter of weeks [1]. SC also have remarkable migratory properties, demonstrated by their ability to move from undamaged muscles, into areas that require repair [2,3]. However, in a disease context, the ability of SC to promote repair is severely hampered, for a number of reasons.…”

Section: Introductionmentioning

confidence: 99%

“…Experimental time-zero (T0) began at the point of myofibre isolation. A total of 250,000 cells of each cell type were seeded into separate single myofibre suspension cultures for 2 h (T0-T2), at 37 • C in 5% CO 2 , allowing for cell-myofibre adherence. At T2, myofibres (with non-muscle cells attached) were transferred to new culture wells with fresh SFCM.…”

Section: Single Myofibre Isolation and Culturementioning

confidence: 99%

“…Briefly, the EDL was carefully dissected and myofibres were dissociated with 0.2% type I collagenase in Dulbecco's modified Eagle medium (DMEM) at 37 • C in 5% CO 2 . Using tapered glass pipettes, individual myofibres were liberated and placed into a suspension culture with single fibre culture medium (SFCM) consisting of DMEM containing GlutaMAXm supplemented with 10% horse serum, 1% penicillin/streptomycin, and 5% chick embryo extract, and were incubated at 37 • C in 5% CO 2 .…”

Section: Single Myofibre Isolation and Culturementioning

confidence: 99%

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Mammalian Skeletal Muscle Fibres Promote Non-Muscle Stem Cells and Non-Stem Cells to Adopt Myogenic Characteristics

Morash

Collins‐Hooper

Mitchell

et al. 2017

Fibers

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Skeletal muscle fibres are unique cells in large animals, often composed of thousands of post-mitotic nuclei. Following skeletal muscle damage, resident stem cells, called satellite cells, commit to myogenic differentiation and migrate to carry out repair. Satellite stem cells migrate on muscle fibres through amoeboid movement, which relies on dynamic cell membrane extension and retraction (blebbing). It is not known whether blebbing is due to the intrinsic properties of satellite cells, or induced by features of the myofibre surface. Here, we determined the influence of the muscle fibre matrix on two important features of muscle regeneration: the ability to migrate and to differentiate down a myogenic lineage. We show that the muscle fibre is able to induce amoeboid movement in non-muscle stem cells and non-stem cells. Secondly, we show that prolonged co-culture on myofibres caused amniotic fluid stem cells and breast cancer cells to express MyoD, a key myogenic determinant. Finally, we show that amniotic fluid stem cells co-cultured on myofibres are able to fuse and make myotubes that express Myosin Heavy Chain.

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Migration of muscle cells

Cited by 61 publications

References 13 publications

Growth Factor Coinjection Improves the Migration Potential of Monkey Myogenic Precursors without Affecting Cell Transplantation Success

Growth Factor Coinjection Improves the Migration Potential of Monkey Myogenic Precursors without Affecting Cell Transplantation Success

Naked DNA expressing two isoforms of hepatocyte growth factor induces collateral artery augmentation in a rabbit model of limb ischemia

Mammalian Skeletal Muscle Fibres Promote Non-Muscle Stem Cells and Non-Stem Cells to Adopt Myogenic Characteristics

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