Migration of mesenchymal cell fated to blastema is necessary for fish fin regeneration

Nakatani, Yuki; Nishidate, Masanobu; Fujita, Mitsuhiro; Kawakami, Koichi

doi:10.1111/j.1440-169x.2007.00977.x

Cited by 23 publications

(21 citation statements)

References 64 publications

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“…2E). Although a previous study has suggested that RE cells are recruited from the proximal epidermal cells (Nakatani et al, 2008), our analysis further showed that many epidermal cells that migrate from the inter-ray region contribute to RE formation.…”

Section: Fate Tracking Of Fn1b + Re Cellscontrasting

confidence: 74%

Heterogeneous fates and dynamic rearrangement of regenerative epidermis-derived cells during zebrafish fin regeneration

et al. 2018

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The regenerative epidermis (RE) is a specialized tissue that plays an essential role in tissue regeneration. However, the fate of the RE during and after regeneration is unknown. In this study, we performed Cre--mediated cell fate tracking and revealed the fates of a major population of the RE cells that express () during zebrafish fin regeneration. Our study showed that these RE cells are mainly recruited from the inter-ray epidermis, and that they follow heterogeneous cell fates. Early recruited cells contribute to initial wound healing and soon disappear by apoptosis, while the later recruited cells contribute to the regenerated epidermis. Intriguingly, many of these cells are also expelled from the regenerated tissue by a dynamic caudal movement of the epidermis over time, and in turn the loss of epidermal cells is replenished by a global self-replication of basal and suprabasal cells in fin. De-differentiation of non-basal epidermal cells into the basal epidermal cells did not occur during regeneration. Overall, our study reveals the heterogeneous fates of RE cells and a dynamic rearrangement of the epidermis during and after regeneration.

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Section: Fate Tracking Of Fn1b + Re Cellscontrasting

confidence: 74%

Heterogeneous fates and dynamic rearrangement of regenerative epidermis-derived cells during zebrafish fin regeneration

et al. 2018

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“…Previous studies have indicated that muscle cells, mesenchymal cells, cartilage cells, nerve cells and some cells embedded in mandible peristeum, contribute to the blastema formation [15], [19], [33]–[34]. Our histological observations showed that nucleated blood cells, fibroblasts, fragmented muscle cells and neural crest-derived pigment cells were included in the lower jaw-regenerating blastema.…”

Section: Discussionsupporting

confidence: 57%

Two Origins of Blastemal Progenitors Define Blastemal Regeneration of Zebrafish Lower Jaw

Wang

Tang

et al. 2012

PLoS ONE

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Zebrafish possess a remarkable ability to regenerate complicated structures by formation of a mass of undifferentiated mesenchymal cells called blastema. To understand how the blastema retains the original structural form, we investigate cellular transitions and transcriptional characteristics of cell identity genes during all stages of regeneration of an amputated lower jaw. We find that mesenchymal blastema originates from multiple sources including nucleated blood cells, fibroblasts, damaged muscle cells and pigment cells. These cells are transformed into two populations of blastemal progenitors: foxi1-expression and isl1-expression, before giving rise to cartilage, bone, and muscle. Time point- based transcriptomal analysis of 45 annotated Hox genes reveal that five 3′-end Hox genes and an equal number of 5′-end Hox genes are activated largely at the stage of blastema reformation. RNA in situ hybridization shows that foxi1 and pax3a are respectively expressed in the presumptive mandible skeletal region and regenerating muscle at 5 dpa. In contrast, hoxa2b and hoxa11b are widely expressed with different domain in chondrogenic blastema and blastema mesenchyme. Knockdown foxi1 changes the expression patterns of sox9a and hoxa2b in chondrogenic blastema. From these results we propose that two origins of blastemal progenitors define blastema skeleton and muscle respecifications through distinct signaling pathways. Meanwhile, the positional identity of blastema reformation is implicated in mesenchymal segmentation and characteristic expression pattern of Hox genes.

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“…These steps do not involve local increases in cell proliferation [6], [7]. However, the epidermal cells, several segments away from the amputation plane, proliferate and migrate towards the wound [11], [12]. Subsequently, a group of highly proliferative mesenchymal cells near the amputation site form a structure called the blastema (Figure S1B) [11], [13], [14].…”

Section: Introductionmentioning

confidence: 99%

Circadian Timing of Injury-Induced Cell Proliferation in Zebrafish

Idda¹,

Kage²,

López‐Olmeda³

et al. 2012

PLoS ONE

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In certain vertebrates such as the zebrafish, most tissues and organs including the heart and central nervous system possess the remarkable ability to regenerate following severe injury. Both spatial and temporal control of cell proliferation and differentiation is essential for the successful repair and re-growth of damaged tissues. Here, using the regenerating adult zebrafish caudal fin as a model, we have demonstrated an involvement of the circadian clock in timing cell proliferation following injury. Using a BrdU incorporation assay with a short labeling period, we reveal high amplitude daily rhythms in S-phase in the epidermal cell layer of the fin under normal conditions. Peak numbers of S-phase cells occur at the end of the light period while lowest levels are observed at the end of the dark period. Remarkably, immediately following amputation the basal level of epidermal cell proliferation increases significantly with kinetics, depending upon the time of day when the amputation is performed. In sharp contrast, we failed to detect circadian rhythms of S-phase in the highly proliferative mesenchymal cells of the blastema. Subsequently, during the entire period of outgrowth of the new fin, elevated, cycling levels of epidermal cell proliferation persist. Thus, our results point to a preferential role for the circadian clock in the timing of epidermal cell proliferation in response to injury.

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Migration of mesenchymal cell fated to blastema is necessary for fish fin regeneration

Cited by 23 publications

References 64 publications

Heterogeneous fates and dynamic rearrangement of regenerative epidermis-derived cells during zebrafish fin regeneration

Heterogeneous fates and dynamic rearrangement of regenerative epidermis-derived cells during zebrafish fin regeneration

Two Origins of Blastemal Progenitors Define Blastemal Regeneration of Zebrafish Lower Jaw

Circadian Timing of Injury-Induced Cell Proliferation in Zebrafish

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