Migration behavior of inorganic anions in micellar electrokinetic capillary chromatography using cationic surfactant

Kaneta, Takashi; Tanaka, Shunitz; Taga, Mitsuhiko; Yoshida, Hitoshi

doi:10.1021/ac00031a017

Cited by 90 publications

(46 citation statements)

References 35 publications

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“…Since pH 2 was used, which is low enough to suppress the electroosmotic flow by not allowing enough deprotonation or ionization of the silinol groups that form the internal surface of the capillary, the V eo term equals zero [21,23,24]. This condition was proven by using a neutral marker that was not observed by the UV detector following 5 h of analysis time.…”

Section: Calculation Of the Local Anesthetic-cd Interaction By Ce Andmentioning

confidence: 99%

Comparison of local anesthetic-cyclodextrin non-covalent complexes using capillary electrophoresis and electrospray ionization mass spectrometry

Alnouti

Bartlett

2002

J. Am. Soc. Mass Spectrom.

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Non-covalent complexes between three derivitized cyclodextrins (CD's) and six local anesthetics were studied using capillary electrophoresis (CE) and electrospray ionization mass spectrometry (ESI-MS). The CE study was performed using the complete filling technique (CFT). A comparison between the migration data from CE and ESI-MS inclusion complex peak abundances was made representing the association between local anesthetics and CD's in the solution and the gas phase, respectively. The results from this study showed comparable behavior of the complexes in the CE and mass spectrometer, indicating similarity in the parameters controlling the stability of these complexes. Therefore, the formation of specific non-covalent complexes, as shown in this study, could be used to predict the behavior of a complexing agent with a substrate in the solution phase by observing data obtained from ESI-MS. (J Am Soc Mass Spectrom 2002, 13, 928 -935)

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Section: Calculation Of the Local Anesthetic-cd Interaction By Ce Andmentioning

confidence: 99%

Comparison of local anesthetic-cyclodextrin non-covalent complexes using capillary electrophoresis and electrospray ionization mass spectrometry

Alnouti

Bartlett

2002

J. Am. Soc. Mass Spectrom.

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“…Besides ILs, other modifiers such as urea and glucose have been applied in MEKC. Urea increases the elution window as well as the solubility of highly hydrophobic solutes in the BGE [16], while glucose, when added to the BGE, enhances resolution [17]. It should be noted that examination of ILs as BGE modifiers is really an examination of the effects of IL component ions since these IL systems are no longer ILs under the conditions examined.…”

Section: Introductionmentioning

confidence: 99%

A comparison of ionic liquids to molecular organic solvents as additives for chiral separations in micellar electrokinetic chromatography

Mwongela

Siminialayi

Fletcher

et al. 2007

J of Separation Science

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A comparison of ionic liquids to molecular organic solvents as additives for chiral separations in micellar electrokinetic chromatographyIn this study, we report the effects of adding ionic liquids (ILs), as compared to adding conventional molecular organic solvents (MOSs), to aqueous buffer solutions containing molecular micelles in the separation of chiral analyte mixtures in micellar EKC (MEKC). The molecular micelle used in this study was polysodium oleyl-L-leucylvalinate (poly-L-SOLV). The ILs were 1-alkyl-3-methylimidazolium tetrafluoroborate, where the alkyl group was ethyl, butyl, hexyl, or octyl. These ILs were chosen due to their hydrophobicity, good solvating, and electrolyte properties. Thus, it was expected that these ILs would have favorable interactions with chiral analytes and not adversely affect the background current. Common CE buffers, mixed with a molecular micelle, and an IL or a MOS, were used for these chiral separations. The buffers containing an IL in the concentration range of 0.02 -0.1 v/v were found to support a reasonable current when an electric field strength of 500 V/cm was applied across the capillary. However, a current break down was observed for the buffers containing more than 60% v/v MOS on application of the above-mentioned electric field. The chiral resolution and selectivity of the analytes were dependent on the concentration and type of IL or MOS used.

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Section: Introductionmentioning

confidence: 99%

“…Selection of an acidic migrating solution is one answer and the EOF will be reduced with the migrating solution. Since cationic surfactants are used to form bi-layers by the dynamic coating of the capillary [1][2][3] and the EOF is reversed as a result, a surface-refining reagent is indispensable in the CZE separation of anionic species.In addition to the cationic surfactants, cationic polyelectrolytes are promising alternatives to reverse the direction of the EOF; poly(1,1-dimethyl-3,5-dimethylenepiperidinium) chromate, hexadimethrine chromate, poly(1,1-dimethyl-3,5-dimethylenepyrrolidinium) chromate, diethylaminoethyl dextran chromate were used as such polyelectrolytes, and the analyte anions were indirectly detected with chromate ions. 4 The cationic polyelectrolytes play an additional role to work as interacting reagent with the analyte anions.…”

mentioning

confidence: 99%

Chitosan as Cationic Polyelectrolyte for the Modification of Electroosmotic Flow and Its Utilization for the Separation of Inorganic Anions by Capillary Zone Electrophoresis

Takayanagi

Motomizu

2006

ANAL. SCI.

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IntroductionIn the analysis of anionic species by capillary zone electrophoresis (CZE), control of the direction and speed of the electroosmotic flow (EOF) is essential, because the direction of the electrophoretic migration of anions is opposite to that of the EOF, when a usual fused silica capillary is used. When the analyte anions possessing large electrophoretic mobility, such as inorganic anions, are supposed to be detected, the electrophoretic mobility of the analytes is larger than the EOF or almost equivalent to it at alkaline solution, and it takes a longer separation time even using the reversal of the electric field. Therefore, the speed of the EOF is usually suppressed or reversed for the analysis of anionic species. Selection of an acidic migrating solution is one answer and the EOF will be reduced with the migrating solution. Since cationic surfactants are used to form bi-layers by the dynamic coating of the capillary [1][2][3] and the EOF is reversed as a result, a surface-refining reagent is indispensable in the CZE separation of anionic species.In addition to the cationic surfactants, cationic polyelectrolytes are promising alternatives to reverse the direction of the EOF; poly(1,1-dimethyl-3,5-dimethylenepiperidinium) chromate, hexadimethrine chromate, poly(1,1-dimethyl-3,5-dimethylenepyrrolidinium) chromate, diethylaminoethyl dextran chromate were used as such polyelectrolytes, and the analyte anions were indirectly detected with chromate ions. 4 The cationic polyelectrolytes play an additional role to work as interacting reagent with the analyte anions. Haddad and his coworkers investigated the ion-exchange mechanism in the CZE separation by simulation and practical resolution improvement, and they proposed ion chromatography-capillary electrophoresis. 5,6 Electrophoretic mobility of some organic anions was reduced by ion-exchange with poly(diallyldimethylammonium)phthalate background electrolyte, and the selectivity among the anions were also developed.Chitosan, an N-deacetylation product of chitin, is one of the useful cationic polyelectrolytes, and has several characteristics such as being made of relatively hydrophilic saccharine groups, safe and biodegradable materials present in nature. Chitosan is soluble in acidic solution with the protonation of the amino groups (pKa = 6.5). 7 Chitin and chitosan oligosaccharides were separated and detected by capillary zone electrophoresis after derivatization with 8-aminopyrene-1,3,6-trisulfonic acid. 8 High-molecular chitosan was also analyzed by CZE. 9 Chitosan has also been used as coating material on fused silica capillary for the separation of basic proteins, 10 and coupled chitosan has been used for both basic and acidic proteins. 11In this study, chitosan as a cationic polyelectrolyte was used to reverse the EOF by dynamic coating of the capillary innerwall, and the capillary was used for the separation of inorganic anions. The interactions with analyte anions were also investigated through the changes in electrophoretic mobility of some ino...

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Migration behavior of inorganic anions in micellar electrokinetic capillary chromatography using cationic surfactant

Cited by 90 publications

References 35 publications

Comparison of local anesthetic-cyclodextrin non-covalent complexes using capillary electrophoresis and electrospray ionization mass spectrometry

Comparison of local anesthetic-cyclodextrin non-covalent complexes using capillary electrophoresis and electrospray ionization mass spectrometry

A comparison of ionic liquids to molecular organic solvents as additives for chiral separations in micellar electrokinetic chromatography

Chitosan as Cationic Polyelectrolyte for the Modification of Electroosmotic Flow and Its Utilization for the Separation of Inorganic Anions by Capillary Zone Electrophoresis

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