Migration and differentiation of mouse embryonic stem cells transplanted into mature cochlea of rats with aminoglycoside-induced hearing loss

Abstract

EGFP-ESCs transplanted into normal cochlea did not migrate, but those in the amikacin-damaged cochlea could survive and migrate into the scala media and the vestibular cisterna. For the first time, we observed that the EGFP-ESCs migrated into the scala media, took the place of the organ of Corti, and formed a structure just like the cochlear tunnel. Some grafted stem cells even expressed myosin VIIa, the molecular marker of hair cells. Some nerve fibers reached to the bottom of the hair cell-like cells. The ESCs migrated into the vestibule and restored the sensory epithelia of the ampullary crest. The number of the transplanted ESCs reduced over the 6 week period of the study.

Retroauricular Approach for Targeted Cochlear Therapy Experiments in Wistar Albino Rats

“…All animals had preoperative prophylactic antibiotic protection (cephazoline 500 mg/kg/day, IM) (8,9). Rats were then placed in a lateral recumbent position.…”

Stem cells in otology: A message ′loud and clear′

“…[4] Also in a study, embryonic stem cells transplanted into the scala tympani could migrate in the aminoglycoside-affected-cochlea and partly restore the structure of sensory epithelia of the inner ear. [5] In a noise-induced ototoxicity animal model, stem cell transplantation resulted in survival and integration of these cells into suprastrial regions of the spiral ligament which significantly ameliorated permanent auditory brainstem response (ABR) threshold shift for click stimuli. [6] Co-culture between inner ear stem cell spheres (which have potential to bear hair cell markers) and the neonatal organ of corti, can help evaluate and potentially optimize transplantation of inner ear stem cells into the cochlea for hair cell regeneration.…”

A hypothesis study on bionic active noise reduction of auditory organs

“…Embryonic stem cells (ESCs) transplanted after druginduced hearing loss could enter the cochlea through a hole in the scala tympani and migrate to the scala media and vestibular cisterna. They could also differentiate into myosin-VIIa-expressing hair cell-like cells [33].…”