MIG1 overexpression causes flocculation in Saccharomyces cerevisiae

Shankar, C. S.; Ramakrishnan, Muthu; Umesh‐Kumar, S.

doi:10.1099/00221287-142-9-2663

Cited by 9 publications

(5 citation statements)

References 13 publications

(4 reference statements)

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“…However, activation of MIG1 in biofilms is revealed by the 325-gene set as well and is therefore independent of whether biofilms were produced under continuous flow. Overexpression of MIG1 in S. cerevisiae is associated with flocculation (28). Whether overexpression of MIG1 in C. albicans biofilms could promote flocculation and hence increase the aggregation of the cells in biofilms remains to be investigated.…”

Section: Discussionmentioning

confidence: 99%

Candida albicans Biofilms: a Developmental State Associated With Specific and Stable Gene Expression Patterns

García-Sánchez¹,

Aubert²,

Iraqui³

et al. 2004

Eukaryot Cell

337

367

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Like many bacteria, yeast species can form biofilms on several surfaces. Candida albicans colonizes the surfaces of catheters, prostheses, and epithelia, forming biofilms that are extremely resistant to antifungal drugs. We have used transcript profiling to investigate the specific properties of C. albicans biofilms. Biofilm and planktonic cultures produced under different conditions of nutrient flow, aerobiosis, or glucose concentration were compared by overall gene expression correlation. Correlation was much higher between biofilms than planktonic populations irrespective of the growth conditions, indicating that biofilm populations formed in different environments display very similar and specific transcript profiles. A first cluster of 325 differentially expressed genes was identified. In agreement with the overrepresentation of amino acid biosynthesis genes in this cluster, Gcn4p, a regulator of amino acid metabolism, was shown to be required for normal biofilm growth. To identify biofilm-related genes that are independent of mycelial development, we studied the transcriptome of biofilms produced by a wild-type, hypha-producing strain and a cph1/cph1 efg1/efg1 strain defective for hypha production. This analysis identified a cluster of 317 genes expressed independently of hypha formation, whereas 86 genes were dependent on mycelial development. Both sets revealed the activation of the sulfur-amino acid biosynthesis pathway as a feature of C. albicans biofilms.

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Section: Discussionmentioning

confidence: 99%

Candida albicans Biofilms: a Developmental State Associated With Specific and Stable Gene Expression Patterns

García-Sánchez¹,

Aubert²,

Iraqui³

et al. 2004

Eukaryot Cell

337

367

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“…Mig1 is responsible for repressing gluconeogenic enzymes, the galactose path‐way, and the maltose pathway. It has been observed that mig1 mutations enhance flocculation but do not affect invasive growth (Shankar et al. , 1996; Cullen and Sprague, 2000).…”

Section: Discussionmentioning

confidence: 99%

Depression of Saccharomyces cerevisiae invasive growth on non‐glucose carbon sources requires the Snf1 kinase

Palecek

Parikh

Huh

et al. 2002

Molecular Microbiology

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Haploid Saccharomyces cerevisiae cells growing on media lacking glucose but containing high concentrations of carbon sources such as fructose, galactose, raffinose, and ethanol exhibit enhanced agar invasion. These carbon sources also promote diploid filamentous growth in response to nitrogen starvation. The enhanced invasive and filamentous growth phenotypes are suppressed by the addition of glucose to the media and require the Snf1 kinase. Mutations in the PGI1 and GND1 genes encoding carbon source utilization enzymes confer enhanced invasive growth that is unaffected by glucose but requires active Snf1. Carbon source does not modulate FLO11 flocculin expression, but enhanced polarized bud site selection is necessary for invasion on certain carbon sources. Interestingly, deletion of SNF1 blocks invasion without affecting bud site selection. Snf1 is also required for formation of spokes and hubs in multicellular mats. To examine glucose repression of invasive growth more broadly, we performed genome-wide microarray expression analysis in wild-type cells growing on glucose and galactose, and snf1 Delta cells on galactose. SNF1 probably mediates glucose repression of multiple genes potentially involved in invasive and filamentous growth. FLO11-independent cell-cell attachment, cell wall integrity, and/or polarized growth are affected by carbon source metabolism. In addition, derepression of cell cycle genes and signalling via the cAMP-PKA pathway appears to depend upon SNF1 activity during growth on galactose.

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“…One pathway implies the Snf1 protein kinase [13]. Other authors have shown that mutation or overexpression of Mig1 protein modi¢es the £occulating properties of S. cerevisiae strains [17], demonstrating that a relation exists mainly between glucose and £occulation. Other authors have shown that mutation or overexpression of Mig1 protein modi¢es the £occulating properties of S. cerevisiae strains [17], demonstrating that a relation exists mainly between glucose and £occulation.…”

Section: Introductionmentioning

confidence: 99%

“…This protein kinase controls Mig1 DNA binding repressor protein which binds the Ssn6-Tup1 corepressor of several glu-cose-repressed genes [14^16]. Other authors have shown that mutation or overexpression of Mig1 protein modi¢es the £occulating properties of S. cerevisiae strains [17], demonstrating that a relation exists mainly between glucose and £occulation. Recently, another pathway has been described in S. cerevisiae implying cAMP-dependent protein kinase (PKA) [18].…”

Section: Introductionmentioning

confidence: 99%

The role of glucose in the Kluyveromyces bulgaricus flocculation phenomenon: transduction by cAMP-dependent protein kinase pathway?

Géhin¹

2001

FEMS Microbiology Letters

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Yeast flocculation appears to be dependent on several culture conditions such as nitrogen or carbon sources. In 0.2% glucose medium Kluyveromyces bulgaricus flocculation intensity is weak (10% at maximum) by comparison with flocculation in 2% glucose medium (85% maximum). Addition of glucose to K. bulgaricus in exponential growth phase in 0.2% glucose medium produced a rapid increase of the flocculation percentage during the 30 min following the addition of glucose. cAMP and 2,4‐dinitrophenol showed similar effects while cAMP‐dependent protein kinase (PKA) inhibitors exhibited an antagonist effect. Moreover, the induction of flocculation did not seem to imply translation of new proteins: cycloheximide had no effect, although growth was inhibited. The induction of flocculation mainly implies ATP hydrolysis for activation or secretion of galactose‐specific receptors as demonstrated by treatment with NaN3. We propose a hypothesis that involves a PKA transduction signal leading to flocculation.

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MIG1 overexpression causes flocculation in Saccharomyces cerevisiae

Cited by 9 publications

References 13 publications

Candida albicans Biofilms: a Developmental State Associated With Specific and Stable Gene Expression Patterns

Candida albicans Biofilms: a Developmental State Associated With Specific and Stable Gene Expression Patterns

Depression of Saccharomyces cerevisiae invasive growth on non‐glucose carbon sources requires the Snf1 kinase

The role of glucose in the Kluyveromyces bulgaricus flocculation phenomenon: transduction by cAMP-dependent protein kinase pathway?

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