Midye ve Karideslerde Rotavirus, Adenovirus ve Astrovirus Varlığının Multiplex Real-time PCR Kullanılarak Araştırılması

Abstract

A: Investigation of Rotavirus, Adenovirus and Astrovirus in mussels and shrimps using multiplex real-time PCR. Kafkas Univ Vet Fak Derg, 24 (5): 761-767, 2018761-767, . DOI: 10.9775/kvfd.2018 Abstract Viruses are one of the most common pathogens transmitted via food. Based on epidemiological evidence, human enteric viruses are considered to be as the most commonly transmitted etiological agents by bivalve shellfish. In Istanbul, the most widely consumed sea products after fish are mussels and shrimps. In this study, the presence of rotavirus, adenovirus and astrovirus were investigated in mussel and shrimp samples consumed by hunting on the shores of Istanbul. For this purpose, a total of 28 groups of shrimp and 52 groups of mussel were collected from different places in Istanbul. Each group was analyzed separately as a sample. Viruses were extracted from the digestive tissue by direct elusion method in a glycine/NaCl, pH 9.5 buffer followed by PEG-6000 precipitation. Multiplex Real-time RT-PCR technique was used to analyze the shellfish samples. Astrovirus was found in 63.46% of the total 52 analyzed mussel samples, whereas adenovirus was found in 46.15%. None of the mussel samples was positive for rotavirus. All tested shrimp samples were negative for all three researched viruses. This is the first report on the prevalence of rotavirus, adenovirus and astrovirus in mussels and shrimps in Turkey. The results show that mussels sold in Istanbul are highly contaminated with adenovirus and astrovirus and this could pose a great threat to public health. Keywords: Mussel, Shrimp, Rotavirus, Adenovirus, Astrovirus, RT-PCR Midye ve Karideslerde Rotavirus, Adenovirus ve Astrovirus Varlığının Multiplex Real-time PCR Kullanılarak Araştırılması ÖzViruslar gıda vasıtasıyla en yaygın bulaşan patojenlerden biridir. Epidemiyolojik bulgulara göre insana ait enterik viruslar kabuklu deniz ürünleri vasıtasıyla en çok aktarılan etiyolojik ajan olarak tanımlanmaktadır. İstanbul'da balıktan sonra en çok tüketilen deniz ürünleri midye ve karidestir. Bu çalışmada, İstanbul kıyılarında avlanarak tüketilen midye ve karides numunelerinde rotavirus, adenovirus ve astrovirus varlığı araştırılmıştır. Bu amaçla toplam 28 karides ve 52 midye grubu İstanbul'un farklı yerlerinden toplanmıştır. Her bir grup ayrı bir numune olarak analize alınmıştır. Viruslar glisin/NaCl, pH 9.5 tamponu kullanarak direkt elüsyon metodu ile doğrudan sindirim dokusundan ayrıştırılmış ve PEG-6000 ile konsantre edilmiştir. Alınan midye ve karides numuneleri Multiplex Real-time PCR yöntemi ile analiz edilmiştir. Toplam çalışılan 52 midye numunesinin %46.15'inde adenovirus ve %63.46'sında astrovirus saptanmıştır. Rotavirus hiçbir midye numunesinde tespit edilmemiştir. Karides numunelerinin hiçbirinde araştırılan virusların hiçbiri saptanmamıştır. Bu, Türkiye'deki midyelerde ve karideslerde rotavirus, adenovirus ve astrovirus prevalansı hakkındaki ilk rapordur. Sonuçlar, İstanbul'da satılan midyelerin adenovirus ve astrovirus ile kontamine olduğunu ve bu durumun halk s...

Seafood associated human pathogenic non-enveloped viruses

“…Choo and Kim (2006) reported that 50.9% of oysters harvested from the wholesale fishing industry in Noryangjin were infected with contagious adenovirus. Ghalyoun and Alçay (2018) detected adenovirus before the fishing season ended in 46.15% of the musk sample from three separate locations in Istanbul. Luz et al (2015) reported that the findings of the analysis revealed substantial bioaccumulation of adenoviruses in shrimp, demonstrating the magnitude of fecal pollution 's effect on aquatic environments.…”