Midtrimester microbial DNA variations in maternal serum of women who experience spontaneous preterm birth

Subramaniam, Akila; Pol, William J. Van Der; Ptacek, Travis; Lobashevsky, Elena; Neely, Cherry; Biggio, Joseph; Lefkowitz, Elliot J.; Morrow, Casey D.; Edwards, Rodney K.

doi:10.1080/14767058.2018.1490721

Cited by 5 publications

(6 citation statements)

References 48 publications

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“…This indicates that the community structure of the blood microbiota in women with PTB differs from that in women with term delivery, even though the microbial community structure does not change during healthy pregnancy. However, a previous study reported that PTB is associated with distinct microbial DNA changes detected in midtrimester maternal serum (Subramaniam et al, 2018). Another study reported the increased richness and diversity of the vaginal microbiome in spontaneous PTB (Freitas et al, 2018).…”

Section: Discussionmentioning

confidence: 94%

Blood Microbial Communities During Pregnancy Are Associated With Preterm Birth

et al. 2019

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Microbial infection of the placenta, amniotic fluid, vaginal canal, and oral cavity is known to significantly contribute to preterm birth (PTB). Although microbes can be translocated into the blood, little is known regarding the blood microbiota during pregnancy. To assess changes in the microbiome during pregnancy, blood samples were obtained 2 or 3 times during pregnancy from a cohort of 45 pregnant women enrolled between 2008 and 2010. To analyze the association with PTB, we conducted a case-control study involving 41 pregnant women upon admission for preterm labor and rupture of membrane (20 with term delivery; 21 with PTB). Bacterial diversity was assessed in number and composition between the first, second, and third trimesters in term delivered women according to 16S rRNA gene amplicon sequencing, and data were analyzed using Quantitative Insight Into Microbial Ecology (QIIME). Taxonomy was assigned using the GreenGenes 8.15.13 database. Dominant microorganisms at the phylum level in all pregnant women were identified as Firmicutes , Proteobacteria , Bacteroidetes , and Actinobacteria . However, the number and composition of bacteria in women with PTB differed from that in women with term delivery. Firmicutes and Bacteroidetes were more abundant in women with PTB than in women with term delivery, while Proteobacteria was less prevalent in women with PTB. At the genus level, Bacteroides , Lactobacillus , Sphingomonas , Fastidiosipila , Weissella , and Butyricicoccus were enriched in PTB samples. These observational results suggest that several taxa in the maternal blood microbiome are associated with PTB. Further studies are needed to confirm the composition of the blood microbiota in women with PTB. Additionally, the mechanism by which pathogenic microbes in maternal blood cause infection and PTB requires further analysis.

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Section: Discussionmentioning

confidence: 94%

Blood Microbial Communities During Pregnancy Are Associated With Preterm Birth

et al. 2019

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“…11 At present, transvaginal cervical tube length measurement is the best method to screen for the risk of premature delivery in pregnancy, but its application is limited, so it is necessary to find better screening indicators. 12 The differentially expressed proteins identified in this present study were mainly from the complement and coagulation cascade pathways. Previous research suggested that the mean platelet volume can predict the early-onset of neonatal sepsis in preterm infants.…”

Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Serum apolipoprotein A-II and alpha-2-antiplasmin levels in midtrimester can be used as predictors of preterm delivery

Huang

Yang

2020

J Int Med Res

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Objective To identify serum protein markers in midtrimester that predict preterm delivery. Methods A retrospective case–control study randomly selected patients that experienced spontaneous preterm birth and healthy control patients that experienced a normal delivery at term. A proteomic analysis was undertaken using the data-independent acquisition method. Results A total of 30 singleton pregnant women were randomly selected from 12 800 pregnant women: 15 women had a spontaneous preterm birth (group Y) and 15 age- and body mass index-matched women gave birth at term (group D). All of the patients provided serum at 15–20 weeks of gestation. A total of 39 differentially expressed proteins were identified. Compared with group D, 24 proteins were upregulated and 15 were downregulated in the preterm group Y. Using Kyoto Encyclopedia of Genes and Genomes pathway enrichment, the 24 upregulated proteins were significantly enriched in the complement and coagulation cascade pathways. Search Tool for the Retrieval of Interacting Genes Furthermore (STRING) analysis showed that apolipoprotein A-II (apoA-II) and alpha-2-antiplasmin (α2-AP), two upregulated proteins, were key nodes in the STRING protein–protein network. Conclusions These findings suggest that apoA-II and α2-AP might be new markers for predicting preterm delivery in the midtrimester.

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“…Many studies focused on the various aspects of relationships between blood dysbiosis and type 2 diabetes mellitus (T2DM), especially as regards the profiles and mechanistic insights (Table 3) [18,22,30,57,58,76]. Amar et al (2011) reported that healthy subjects whose blood contained a lower amount of Proteobacteria, as well as a higher amount of Actinobacteria, may have a reduced chance of the development of T2DM in the future [22]; however, higher blood 16s rRNA concentration in a healthy person might lead to T2DM [22].…”

Section: Age-related Metabolic Diseases and Profiles Of Blood Dysbiosismentioning

confidence: 99%

“…Among patients with morbid obesity, Anhe et al (2020) found that Enterobacteriaceae, specifically Escherichia-Shigella, and Neisseriaceae were increased in the patients who had T2DM [57]. However, Massier et al (2020) observed the differences at only the genus level; patients with morbid obesity and T2DM tended to have a higher level of Tahibacter with lower levels of Delftia, Lactobacillus, and Lactococcus when compared to subjects with morbid obesity without T2DM [58]. In patients with obesity, blood dysbiosis was likely to show an increase in Propionibactereles, Sphingomonadales, and Norcardioides [30].…”

Section: Age-related Metabolic Diseases and Profiles Of Blood Dysbiosismentioning

confidence: 99%

Human Blood Bacteriome: Eubiotic and Dysbiotic States in Health and Diseases

Suparan

Sriwichaiin

Chattipakorn

2022

Cells

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The human gut microbiome is acknowledged as being associated with homeostasis and the pathogenesis of several diseases. Conventional culture techniques are limited in that they cannot culture the commensals; however, next-generation sequencing has facilitated the discovery of the diverse and delicate microbial relationship in body sites and blood. Increasing evidence regarding the blood microbiome has revolutionized the concept of sterility and germ theory in circulation. Among the types of microbial communities in the blood, bacteriomes associated with many health conditions have been thoroughly investigated. Blood bacterial profiles in healthy subjects are identified as the eubiotic blood bacteriome, whereas the dysbiotic blood bacteriome represents the change in bacterial characteristics in subjects with diseases showing deviations from the eubiotic profiles. The blood bacterial characteristics in each study are heterogeneous; thus, the association between eubiotic and dysbiotic blood bacteriomes and health and disease is still debatable. Thereby, this review aims to summarize and discuss the evidence concerning eubiotic and dysbiotic blood bacteriomes characterized by next-generation sequencing in human studies. Knowledge pertaining to the blood bacteriome will transform the concepts around health and disease in humans, facilitating clinical implementation in the near future.

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Midtrimester microbial DNA variations in maternal serum of women who experience spontaneous preterm birth

Abstract: SPTB is associated with distinct microbial DNA changes detected in midtrimester maternal serum.

Cited by 5 publications

References 48 publications

Blood Microbial Communities During Pregnancy Are Associated With Preterm Birth

Blood Microbial Communities During Pregnancy Are Associated With Preterm Birth

Serum apolipoprotein A-II and alpha-2-antiplasmin levels in midtrimester can be used as predictors of preterm delivery

Human Blood Bacteriome: Eubiotic and Dysbiotic States in Health and Diseases

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