Middle cerebral artery occlusion in the mouse by intraluminal suture coated with poly-l-lysine: neurological and histological validation

Belayev, Ludmila; Busto, Raul; Zhao, Weizhao; Fernandez, Guillermo; Ginsberg, Myron D.

doi:10.1016/s0006-8993(99)01528-0

Cited by 136 publications

(108 citation statements)

References 23 publications

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“…Middle Cerebral Artery Occlusion, In Vivo Model of Ischemic Preconditioning, and Determination of the Volume of the Ischemic Lesion Transient occlusion of the middle cerebral artery (tMCAO) was induced with a 6 to 0 silk suture advanced from the external carotid artery into the internal carotid artery until the origin of the middle cerebral artery, as described elsewhere (Belayev et al, 1999). Briefly, a nylon monofilament (6 to 0, Ethicon, Issy Les Moulineaux, France), coated with silicone, was introduced through the external carotid artery and advanced up to the origin of the middle cerebral artery.…”

Section: In Vitro Model Of Preconditioningmentioning

confidence: 99%

Tissue-Type Plasminogen Activator has a Neuroprotective Effect in the Ischemic Brain Mediated by Neuronal TNF-α

Haile

Echeverry

et al. 2011

J Cereb Blood Flow Metab

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Cerebral cortical neurons have a heightened sensitivity to hypoxia and their survival depends on their ability to accommodate to changes in the concentration of oxygen in their environment. Tissuetype plasminogen activator (tPA) is a serine proteinase that activates the zymogen plasminogen into plasmin. Hypoxia induces the release of tPA from cerebral cortical neurons, and it has been proposed that tPA mediates hypoxic and ischemic neuronal death. Here, we show that tPA is devoid of neurotoxic effects and instead is an endogenous neuroprotectant that renders neurons resistant to the effects of lethal hypoxia and ischemia. We present in vitro and in vivo evidence indicating that endogenous tPA and recombinant tPA induce the expression of neuronal tumor necrosis factor-a. This effect, mediated by plasmin and the N-methyl-D-aspartate receptor, leads to increased expression of the cyclin-dependent kinase inhibitor p21 and p21-mediated development of early hypoxic and ischemic tolerance.

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Section: In Vitro Model Of Preconditioningmentioning

confidence: 99%

Tissue-Type Plasminogen Activator has a Neuroprotective Effect in the Ischemic Brain Mediated by Neuronal TNF-α

Haile

Echeverry

et al. 2011

J Cereb Blood Flow Metab

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“…Another 6 to 0 silk suture was tied loosely around ECA close to the origin at the common carotid artery. A blunted 5 to 0 monofilament nylon suture coated with poly-L-lysine (0.1% in deionized water, Sigma Inc., St Louis, MO, USA) (Belayev et al, 1999) was introduced into a small incision on ECA and then advanced into the circle of Willis, and finally to the origin of the middle cerebral artery. The silk suture around the ECA stump was tied tightly to prevent bleeding and secure the nylon suture.…”

Section: Middle Cerebral Artery Occlusion and Reperfusionmentioning

confidence: 99%

Cannabinoid CB₂Receptor Activation Decreases Cerebral Infarction in a Mouse Focal Ischemia/Reperfusion Model

Zhang

Martin

Adler

et al. 2007

J Cereb Blood Flow Metab

161

152

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Cannabinoid CB 2 Receptor (CB 2 ) activation has been shown to have immunomodulatory properties without psychotropic effects. The hypothesis of this study is that selective CB 2 agonist treatment can attenuate cerebral ischemia/reperfusion injury. Selective CB 2 agonists (O-3853, O-1966) were administered intravenously 1 h before transient middle cerebral artery occlusion (MCAO) or 10 mins after reperfusion in male mice. Leukocyte/endothelial interactions were evaluated before MCAO, 1 h after MCAO, and 24 h after MCAO via a closed cranial window. Cerebral infarct volume and motor function were determined 24 h after MCAO. Administration of the selective CB 2 agonists significantly decreased cerebral infarction (30%) and improved motor function (P < 0.05) after 1 h MCAO followed by 23 h reperfusion in mice. Transient ischemia in untreated animals was associated with a significant increase in leukocyte rolling and adhesion on both venules and arterioles (P < 0.05), whereas the enhanced rolling and adhesion were attenuated by both selective CB 2 agonists administered either at 1 h before or after MCAO (P < 0.05). CB 2 activation is associated with a reduction in white blood cell rolling and adhesion along cerebral vascular endothelial cells, a reduction in infarct size, and improved motor function after transient focal ischemia.

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“…The distal MCA was electrocoagulated and cut with a technique [27] modified for mouse from Tamamura [28,29]. This procedure causes a large infarct involving cortical and subcortical zones [30]. Two months after the MCAO the animals were terminally anaesthetized and perfused by 4% buffered paraformaldehyde.…”

Section: Animal Experimentsmentioning

confidence: 99%

Sensitive detection of GFP utilizing tyramide signal amplification to overcome gene silencing

Tóth

Shahar

Leker

et al. 2007

Experimental Cell Research

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The green fluorescent protein (GFP) is among the most commonly used expression markers in biology. GFP-tagged cells have played a particularly important role in studies of cell lineage. Sensitive detection of GFP is crucially important for such studies to be successful, and problems with detection may account for discrepancies in the literature regarding the possible fate choices of stem cells. Here we describe a very sensitive technique for visualization of GFP. Using it we can detect about 90% of cells of donor origin while we could only see about 50% of these cells when we employ the methods that are in general use in other laboratories. In addition, we provide evidence that some cells permanently silence GFP expression. In the case of the progeny of bone marrow stem cells, it appears that the more distantly related they are to their precursors, the more likely it is that they will turn off the lineage marker.

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Middle cerebral artery occlusion in the mouse by intraluminal suture coated with poly-l-lysine: neurological and histological validation

Cited by 136 publications

References 23 publications

Tissue-Type Plasminogen Activator has a Neuroprotective Effect in the Ischemic Brain Mediated by Neuronal TNF-α

Tissue-Type Plasminogen Activator has a Neuroprotective Effect in the Ischemic Brain Mediated by Neuronal TNF-α

Cannabinoid CB₂Receptor Activation Decreases Cerebral Infarction in a Mouse Focal Ischemia/Reperfusion Model

Sensitive detection of GFP utilizing tyramide signal amplification to overcome gene silencing

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Middle cerebral artery occlusion in the mouse by intraluminal suture coated with poly-l-lysine: neurological and histological validation

Cited by 136 publications

References 23 publications

Tissue-Type Plasminogen Activator has a Neuroprotective Effect in the Ischemic Brain Mediated by Neuronal TNF-α

Tissue-Type Plasminogen Activator has a Neuroprotective Effect in the Ischemic Brain Mediated by Neuronal TNF-α

Cannabinoid CB2Receptor Activation Decreases Cerebral Infarction in a Mouse Focal Ischemia/Reperfusion Model

Sensitive detection of GFP utilizing tyramide signal amplification to overcome gene silencing

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Product

Resources

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Cannabinoid CB₂Receptor Activation Decreases Cerebral Infarction in a Mouse Focal Ischemia/Reperfusion Model