Mid-winter ultrastructural changes in the vegetative embryonic shoot 
of Norway spruce [<i>Picea abies</i> (L.) Karst.]

Guzicka, Marzenna; Woźny, Adam

doi:10.1051/forest:2003039

Cited by 2 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar to seeds, buds of perennial species establish dormancy, partially desiccate to tolerate frost, and accumulate LDs. Indeed, the presence of LDs in buds has been reported in several perennial species such as birch (Rinne et al, 1999;Rinne et al, 2001), Norway spruce (Guzicka and Wozny, 2003;Guzicka et al, 2018), aspen and poplar (Sagisaka, 1992;Rinne et al, 2011;Veerabagu et al, 2020;Veerabagu et al, 2021), willow (Berggren, 1984), pine (Jordy, 2004) and Cunninghamia lanceolate . LD abundancy is described to vary during winter dormancy progression (Rinne et al, 2001;Sutinen et al, 2009;Sutinen et al, 2012).…”

Section: Ld Abundancy In Buds Of Perennial Trees Varies During the Co...mentioning

confidence: 89%

Lipid droplets are versatile organelles involved in plant development and plant response to environmental changes

Bouchnak,

Coulon,

Salis

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Since decades plant lipid droplets (LDs) are described as storage organelles accumulated in seeds to provide energy for seedling growth after germination. Indeed, LDs are the site of accumulation for neutral lipids, predominantly triacylglycerols (TAGs), one of the most energy-dense molecules, and sterol esters. Such organelles are present in the whole plant kingdom, from microalgae to perennial trees, and can probably be found in all plant tissues. Several studies over the past decade have revealed that LDs are not merely simple energy storage compartments, but also dynamic structures involved in diverse cellular processes like membrane remodeling, regulation of energy homeostasis and stress responses. In this review, we aim to highlight the functions of LDs in plant development and response to environmental changes. In particular, we tackle the fate and roles of LDs during the plant post-stress recovery phase.

show abstract

Section: Ld Abundancy In Buds Of Perennial Trees Varies During the Co...mentioning

confidence: 89%

Lipid droplets are versatile organelles involved in plant development and plant response to environmental changes

Bouchnak,

Coulon,

Salis

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

Molecular and structural changes in vegetative buds of Norway spruce during dormancy in natural weather conditions

et al. 2017

View full text Add to dashboard Cite

The dormancy and the growth of trees in temperate climates are synchronized with seasons. Preparation for dormancy and its proper progression are key for survival and development in the next season. Using a unique approach that combined microscopy and proteomic methods, we investigated changes in Norway spruce (Picea abies (L.) H. Karst.) embryonic shoots during four distinct stages of dormancy in natural weather conditions. We identified 13 proteins that varied among dormancy stages, and were linked to regulation of protein level; functioning of chloroplasts and other plastids; DNA and RNA regulation; and oxidative stress. We also found a group of five proteins, related to cold hardiness, that did not differ in expression among stages of dormancy, but had the highest abundancy level. Ultrastructure of organelles is tightly linked to their metabolic activity, and hence may indicate dormancy status. The observed ultrastructure during endodormancy was stable, whereas during ecodormancy, the structural changes were dynamic and related mainly to nucleus, plastids and mitochondria. At the ultrastructural level, the lack of starch and the presence of callose in plasmodesmata in all regions of embryonic shoot were indicators of full endodormancy. At the initiation of ecodormancy, we noted an increase in metabolic activity of organelles, tissue-specific starch hyperaccumulation and degradation. However, in proteomic analysis, we did not find variation in expression of proteins related to starch degradation or to symplastic isolation of cells. The combination of ultrastructural and proteomic methods gave a more complete picture of vegetative bud dormancy than either of them applied separately. We found some changes at the structural level, but not their analogues in the proteome. Our study suggests a very important role of plastids' organization and metabolism, and their protection in the course of dormancy and during the shift from endo- to ecodormancy and the acquisition of growth competence.

show abstract

Mid-winter ultrastructural changes in the vegetative embryonic shoot of Norway spruce [Picea abies (L.) Karst.]

Cited by 2 publications

References 12 publications

Lipid droplets are versatile organelles involved in plant development and plant response to environmental changes

Lipid droplets are versatile organelles involved in plant development and plant response to environmental changes

Molecular and structural changes in vegetative buds of Norway spruce during dormancy in natural weather conditions

Contact Info

Product

Resources

About