Mid‐infrared spectroscopy on skin using a silver halide fibre probe <i>in vivo</i>

Ring, Annika P; Schreiner, Volker; Wenck, Horst; Wittern, K. P.; Küpper, Lukas; Keyhani, R.

doi:10.1111/j.0909-725x.2006.00130.x

Cited by 10 publications

(7 citation statements)

References 6 publications

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“…They can be especially useful for evaluation of samples that cannot be analyzed using XRD, such as very small mineral amounts, and intact tissues and thin tissue sections. Moreover, when combined with portable Raman and FT-IR spectrometers using fiber optics probes, [57][58][59] these approaches may be useful for in situ evaluation of bone mineral crystallinity in a variety of scenarios. The vibrational spectroscopy approaches shown in this study can be readily implemented in many biomineralization, archeological and orthopedic studies, potentially contributing to the bone research field and for forensic and clinically relevant applications.…”

Section: Assessment and Recommendationmentioning

confidence: 99%

Validated Approaches for Quantification of Bone Mineral Crystallinity Using Transmission Fourier Transform Infrared (FT-IR), Attenuated Total Reflection (ATR) FT-IR, and Raman Spectroscopy

et al. 2018

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Bone mineral crystallinity is an important factor determining bone quality and strength. The gold standard method to quantify crystallinity is X-ray diffraction (XRD), but vibrational spectroscopic methods present powerful alternatives to evaluate a greater variety of sample types. We describe original approaches by which transmission Fourier transform infrared (FT-IR), attenuated total reflection (ATR) FT-IR, and Raman spectroscopy can be confidently used to quantify bone mineral crystallinity. We analyzed a range of biological and synthetic apatite nanocrystals (10-25 nm) and found strong correlations between different spectral factors and the XRD determination of crystallinity. We highlight striking differences between FT-IR spectra obtained by transmission and ATR. In particular, we show for the first time the absence of the 1030 cm crystalline apatite peak in ATR FT-IR spectra, which excludes its use for analyzing crystallinity using the traditional 1030/1020 cm ratio. The νPO splitting ratio was also not adequate to evaluate crystallinity using ATR FT-IR. However, we established original approaches by which ATR FT-IR can be used to determine apatite crystallinity, such as the 1095/1115 and 960/1115 cm peak ratios in the second derivative spectra. Moreover, we found a simple unified approach that can be applied for all three vibrational spectroscopy modalities: evaluation of the νPO peak position. Our results allow the recommendation of the most reliable analytical methods to estimate bone mineral crystallinity by vibrational spectroscopy, which can be readily implemented in many biomineralization, archeological and orthopedic studies. In particular, we present a step forward in advancing the use of the increasingly utilized ATR FT-IR modality for mineral research.

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Section: Assessment and Recommendationmentioning

confidence: 99%

Validated Approaches for Quantification of Bone Mineral Crystallinity Using Transmission Fourier Transform Infrared (FT-IR), Attenuated Total Reflection (ATR) FT-IR, and Raman Spectroscopy

et al. 2018

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“…In addition to TED-FTIR, new IR techniques have been introduced such as opto-thermal radiometry and mid-IR spectroscopy. 60,61 In contrast to FTIR-ATR techniques, Raman spectroscopy represents a more recent in-vivo approach for non-invasive determination of percutaneous penetration of chemicals. Development of confocal Raman microspectroscopy (CRM) is of particular importance for wider application of this technique.…”

Section: Spectroscopic Methodsmentioning

confidence: 99%

Methods for measuring in-vivo percutaneous absorption in humans

Kežić

2008

Hum Exp Toxicol

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In-vivo human data on percutaneous absorption are scarce, although they are indispensable for health risk assessment of dermal exposure. In addition, they are considered to be the gold standard for the evaluation of in-vitro systems as well as predictive mathematical models. Dermal absorption in vivo can be assessed using different approaches. The most used methods for determination of in-vivo dermal absorption are the measurement of the parent chemical and/or its metabolite level in biological material, the microdialysis technique and stratum corneum tape stripping. Recently, the non-invasive spectrophotometric methods based on infrared and Raman spectroscopy showed themselves as promising tools for studying percutaneous absorption though these approaches are still in their developmental stages and requires further optimization and validation. The aim of this article is to review different methods for determination of percutaneous absorption in vivo in humans. The advantages and limitations are discussed with respect to generating data for comparison with in-vitro or predictive mathematical models or health risk assessment of chemicals. Furthermore, the importance of the volunteer experiments in generating relevant data for human risk assessment as well as for the development and implementation of biological monitoring in occupational settings will be addressed.

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“…Our previous works have illustrated the feasibility of discrimination of human kidney , or colon tumors using near-infrared (NIR), IR, and fluorescence spectroscopy as well as a LED-based sensor equipped with a fiber probe . Although ATR spectroscopy is not optimal for the analysis of solid objects, its application for the analysis of biological tissues by fiber-based probes is one of the most important exceptions …”

Section: Introductionmentioning

confidence: 99%

Fiber Probe for Simultaneous Mid-Infrared and Fluorescence Spectroscopic Analysis

et al. 2021

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A multispectral fiber optic probe has been developed that enables simultaneous analysis of various liquid and solid samples using attenuated total reflection mid-infrared spectroscopy and fluorimetry. The probe design was optimized using ray-tracing simulation of the light propagation. Technical evaluation of the probe has confirmed its output signal quality that was comparable to that of respective probes for single methods. The capability of the probe to deliver complementary chemical information from the same measurement point has been illustrated using model samples of biological tissue. Qualitative analysis of the biological tissue is one of the most important applications of the developed multispectral probe.

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Mid‐infrared spectroscopy on skin using a silver halide fibre probe in vivo

Abstract: Using the particular light guide, we were able to measure for the first time the effects of lip care products on lips directly. Furthermore, water binding and glycerol content of the skin could be determined simultaneously, as well as the replenishment of lipids by lipid-enriched bath oil.

Cited by 10 publications

References 6 publications

Validated Approaches for Quantification of Bone Mineral Crystallinity Using Transmission Fourier Transform Infrared (FT-IR), Attenuated Total Reflection (ATR) FT-IR, and Raman Spectroscopy

Validated Approaches for Quantification of Bone Mineral Crystallinity Using Transmission Fourier Transform Infrared (FT-IR), Attenuated Total Reflection (ATR) FT-IR, and Raman Spectroscopy

Methods for measuring in-vivo percutaneous absorption in humans

Fiber Probe for Simultaneous Mid-Infrared and Fluorescence Spectroscopic Analysis

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