Microwell Regulation of Pluripotent Stem Cell Self-Renewal and Differentiation

Hsiao, Cheston; Palecek, Sean P.

doi:10.1007/s12668-012-0050-9

Cited by 18 publications

(15 citation statements)

References 80 publications

(95 reference statements)

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“…The shape of the microwell can be modified into different shapes, including rectangles and triangles (3). Various cell types have been applied to microwells, including embryonic stem cells, islet cells and hepatocytes (5,17,18).…”

Section: Discussionmentioning

confidence: 99%

“…The fates of stem cells, including survival, self-renewal and differentiation, are regulated by chemical and mechanical stimuli presented in their three-dimensional microenvironment (3). The majority of stem cell studies have been performed on two-dimensional substrates (3).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, poly(ethylene glycol) diacrylate, polymethylmethacrylate, poly(N-isopropylacrylamide) and polyester have been applied for the culture platform, while bypassing lithography (8)(9)(10). A previous study proved that three-dimensional culture systems have demonstrated the importance of intercellular interactions in regulating stem cell self-renewal and differentiation (3).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests

Lee

Yeo²,

Kim

et al. 2015

Biomedical Reports

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Abstract. Human mesenchymal stem cells have previously been isolated and characterized from the gingiva, and gingiva-derived stem cells have been applied for tissue engineering purposes. The present study was performed to generate size-controllable stem cell spheroids using concave microwells. Gingiva-derived stem cells were isolated, and the stem cells of 1x10 5 (group A) or 2x10 5 (group B) cells were seeded in polydimethylsiloxane-based, concave micromolds with 600 µm diameters. The morphology of the microspheres was viewed under an inverted microscope, and the changes in the diameter and cell viability were analyzed. The gingiva-derived stem cells formed spheroids in the concave microwells. The diameters of the spheroids were larger in group A compared to group B. No significant changes in shape or diameter were noted with increases in incubation time. Cell viability was higher in group B at each time point when compared with group A. Within the limits of the study, the size-controllable stem cell spheroids could be generated from gingival cells using microwells. The shape of the spheroids and their viability were clearly maintained during the experimental periods.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests

Lee

Yeo²,

Kim

et al. 2015

Biomedical Reports

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show abstract

“…These various determinants such as growth factors and epigenetic modifiers are constantly connected to stem cells, thus regulating their ability for self-renewal, proliferation, and differentiation. 45,47,150 However, to assess characteristics of a stem cell, we need technical access to different time points of the same stem cell or its lineages.…”

Section: Figmentioning

confidence: 99%

Technology Advancement for Integrative Stem Cell Analyses

Jeong

Choi

Lee

2014

Tissue Engineering Part B: Reviews

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Scientists have endeavored to use stem cells for a variety of applications ranging from basic science research to translational medicine. Population-based characterization of such stem cells, while providing an important foundation to further development, often disregard the heterogeneity inherent among individual constituents within a given population. The population-based analysis and characterization of stem cells and the problems associated with such a blanket approach only underscore the need for the development of new analytical technology. In this article, we review current stem cell analytical technologies, along with the advantages and disadvantages of each, followed by applications of these technologies in the field of stem cells. Furthermore, while recent advances in micro/nano technology have led to a growth in the stem cell analytical field, underlying architectural concepts allow only for a vertical analytical approach, in which different desirable parameters are obtained from multiple individual experiments and there are many technical challenges that limit vertically integrated analytical tools. Therefore, we propose-by introducing a concept of vertical and horizontal approach-that there is the need of adequate methods to the integration of information, such that multiple descriptive parameters from a stem cell can be obtained from a single experiment.

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“…To this end, various materials‐based culture platforms have been engineered to systematically study how microenvironmental contributions, such as substrate stiffness, extracellular matrix (ECM) proteins, scaffolding, and mechanical stimuli, affect ESC behavior . 3‐D microwell cell culture platforms have been utilized to modulate cell–cell contact, intercellular, and autoregulatory signaling that occur in hESCs and during differentiation, particularly in embryoid body (EB)‐based differentiation . Some studies have demonstrated that pro‐pluripotency autoregulation can be accentuated in various configurations of 3‐D microwell culture .…”

Section: Introductionmentioning

confidence: 99%

Effects of 3‐D microwell culture on initial fate specification in human embryonic stem cells

et al. 2014

Self Cite

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Several studies have demonstrated that 3D culture systems influence human embryonic stem cell (hESC) phenotypes and fate choices. However, the effect that these microenvironmental changes have on signaling pathways governing hESC behaviors is not well understood. Here, we have used a 3D microwell array to investigate differences in activation of developmental pathways between 2D and 3D cultures of both undifferentiated hESCs and hESCs undergoing initial differentiation in embryoid bodies (EBs). We observed increased induction into mesoderm and endoderm and differences in expression of genes from multiple signaling pathways that regulate development, including Wnt/β-catenin, TGF-β superfamily, Notch and FGF during EB-mediated differentiation, in 3D microwells as compared with the 2D substrates. In undifferentiated hESCs, we also observed differences in epithelial-mesenchymal transition phenotypes and the TGFβ/BMP pathway between cultures in 3D and 2D. These results illustrate that 3D culture influences multiple pathways that may regulate the differentiation trajectories of hESCs.

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Microwell Regulation of Pluripotent Stem Cell Self-Renewal and Differentiation

Cited by 18 publications

References 80 publications

Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests

Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests

Technology Advancement for Integrative Stem Cell Analyses

Effects of 3‐D microwell culture on initial fate specification in human embryonic stem cells

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