Microwave, ultrasound, thermal treatments, and bead milling as intensification techniques for extraction of lipids from oleaginous Yarrowia lipolytica yeast for a biojetfuel application

Abstract

In the present work, two different ways of lipids extraction from Yarrowia lipolytica yeast were investigated in order to maximize the extraction yield. Firstly, various modern techniques of extraction including ultrasound, microwave, and bead milling were tested to intensify the efficiency of lipid recovery. Secondly, several pretreatments such as freezing/defrosting, cold drying, bead milling, and microwave prior two washing of mixture solvent of chloroform:methanol (1:2, v/v) were study to evaluate the impact on lipid recovery. All these treatments were compared to conventional maceration, in terms of lipids extraction yield and lipid composition analysis. The main result of this study is the large difference of lipid recovery among treatments and the alteration of lipids profile after microwave and ultrasound techniques.

Influence of Roasting on Sensory, Antioxidant, Aromas, and Physicochemical Properties of Carob Pod Powder (Ceratonia siliquaL.)

“…After extraction, the extract was concentrated until being dried by solvent evaporation under vacuum (Laborota 4001, Heidolph, Germany) and finally, it was conserved at 4 ∘ C before analysis. Detailed analysis has been explained and detailed in our previous publication (Meullemiestre et al 2016) [49].…”

“…Lipid classes of each carob extract were identified and quantified against those of corresponding lipid standards. Detailed analysis has been explained and detailed in our previous publication Meullemiestre et al 2016 [49].…”

“…Triheptadecanoin (C17:0 TAG) was used as internal standard. Detailed analysis has been explained and detailed in our previous publication (Meullemiestre et al) 2016 [49].…”

“…FAMEs were identified by retention time and comparison with purified FAME standards (Sigma Co., USA). Detailed analysis has been explained and detailed in our previous publication (Meullemiestre et al) 2016 [49].…”

“Bligh and Dyer” and Folch Methods for Solid–Liquid–Liquid Extraction of Lipids from Microorganisms. Comprehension of Solvatation Mechanisms and towards Substitution with Alternative Solvents

“…They have been reviewed by Iverson and coworkers [21]. Among them, the use of ultrasound [1,22,23,24], microwaves [16,22,23,25,26], heat [25,27], pressure [27,28] or beads [24,27] were often raised to improve the efficiency of lipid recovery from various tissues such as bacteria [23], yeasts [29] or microalgae [30] (supplementary material). However, all these methods were using solvents of fossil origin issued from petrol.…”