Microwave Technology in Immunohistochemistry: Application to Avidin-Biotin Staining of Diverse Antigens

Takes, Peter A.; Kohrs, Joan; Krug, Robert M.; Kewley, Sharyl

doi:10.1179/014788889794651960

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“…However, the immunostaining obtained from a 3-minute incubation at 37°C in the thermal cycler followed by 2-minute incubation without heating was much weaker than that seen with MWs. Takes et al [44] similarly demonstrated that 7-s MW irradiation followed by 5-min room temperature incubation for each step of the avidin-biotin peroxidase complex procedure produced good immunolabelling. The droplet temperature will rise no more than 5°C following 7-s irradiation at 100% power in a 850-watt oven so that temperature was not a significant component of the accelerated reaction.…”

Section: Discussionmentioning

confidence: 95%