Recently, it has been shown that the chiral magnetic insulator Cu2OSeO3 hosts skyrmions in two separated pockets in temperature and magnetic field phase space. It has also been shown that the predominant stabilization mechanism for the low-temperature skyrmions (LTS) phase is the crystalline anisotropy in contrast to temperature fluctuations, which stabilize the well established high-temperature skyrmion (HTS) lattice. Here, we report on the gigahertz dynamics in the LTS phase in Cu2OSeO3. The LTS phase is populated via a field cycling protocol with the static magnetic field applied parallel to the h100i crystalline direction of plate and cuboid-shaped bulk crystals. By analyzing temperature-dependent broadband spectroscopy data, clear evidence of low-temperature skyrmion excitations with clockwise (CW), counterclockwise (CCW), and breathing mode (BR) character at temperatures below T = 40 K are shown. We find that the modes’ intensities can be tuned with the number of field-cycles below the saturation field, and by tracking the resonance frequencies, the LTS phase diagram can be established. From our experiments, we conclude that the LTS phase is well separated from the high-temperature phase. Furthermore, by monitoring the strength of the observed hybridization between a dark CW mode and the BR as a function of temperature for the two differently shaped crystals, we unambiguously conclude that the magnetocrystalline anisotropy governs the hybridization.