Microwave sensor for detection of E. coli in water

Oberoi, Sudhi; Daya, K. S.; Tirumalai, Prem Saran

doi:10.1109/icsenst.2012.6461753

Cited by 7 publications

(8 citation statements)

References 6 publications

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“…Therefore, the permittivity of bacterial cells on the influence of external electric fields depends on their species, type (gram-positive or gram-negative) [107,109,110], as well as on the electrical conductivity and permittivity of various intracellular components of bacteria. In addition, they may depend on the physiological state of bacteria (metabolic activity level, degree of viability), as well as on living conditions and the state of their hydration [111][112][113].…”

Section: Microwave Label-free Biosensorsmentioning

confidence: 99%

“…Unlike optical biosensors, which measure the optical properties of biomolecules (for example, by interferometry, PPR or SERS), MW-biosensors use capacitive sensing (low-frequency or microwave) to measure permittivity using counter capacitors, resonators, and microstrip structures [114,115]. The high sensitivity and specificity of MW-biosensors allow not only to identify biological agents but also to assess the physiological state of bacterial cells, to differentiate living cells from dead cells [69,113,114].…”

Section: Microwave Label-free Biosensorsmentioning

confidence: 99%

“…Convincing evidence of the high detection rate and impressive sensitivity of MW-biosensors was given by S. Oberoi with colleagues. A biosensor of this type constructed by the authors detected and identified 4-5 min before 1-2 colony forming units (CFU) of E. coli in water samples [113].…”

Section: Microwave Label-free Biosensorsmentioning

confidence: 99%

“…Of undoubted value and high relevance is the work on the study of the possibility of label-free microwave sensors. The authors tested a MW-sensor that detected different concentrations of E. coli, reaching 10 3 CFU/mL with biofunctionalization of the sensor by polyclonal antibodies [69,113].…”

Section: Microwave Label-free Biosensorsmentioning

confidence: 99%

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Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

et al. 2020

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Infections pose a serious global public health problem and are a major cause of premature mortality worldwide. One of the most challenging objectives faced by modern medicine is timely and accurate laboratory-based diagnostics of infectious diseases. Being a key factor of timely initiation and success of treatment, it may potentially provide reduction in incidence of a disease, as well as prevent outbreak and spread of dangerous epidemics. The traditional methods of laboratory-based diagnostics of infectious diseases are quite time- and labor-consuming, require expensive equipment and qualified personnel, which restricts their use in case of limited resources. Over the past six decades, diagnostic technologies based on lateral flow immunoassay (LFIA) have been and remain true alternatives to modern laboratory analyzers and have been successfully used to quickly detect molecular ligands in biosubstrates to diagnose many infectious diseases and septic conditions. These devices are considered as simplified formats of modern biosensors. Recent advances in the development of label-free biosensor technologies have made them promising diagnostic tools that combine rapid pathogen indication, simplicity, user-friendliness, operational efficiency, accuracy, and cost effectiveness, with a trend towards creation of portable platforms. These qualities exceed the generally accepted standards of microbiological and immunological diagnostics and open up a broad range of applications of these analytical systems in clinical practice immediately at the site of medical care (point-of-care concept, POC). A great variety of modern nanoarchitectonics of biosensors are based on the use of a broad range of analytical and constructive strategies and identification of various regulatory and functional molecular markers associated with infectious bacterial pathogens. Resolution of the existing biosensing issues will provide rapid development of diagnostic biotechnologies.

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Section: Microwave Label-free Biosensorsmentioning

confidence: 99%

Section: Microwave Label-free Biosensorsmentioning

confidence: 99%

Section: Microwave Label-free Biosensorsmentioning

confidence: 99%

Section: Microwave Label-free Biosensorsmentioning

confidence: 99%

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Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

et al. 2020

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“…Microwave sensing is recently developing for clinical microbiology practices [23][24][25][26] . Microwave resonators have shown to be highly sensitive in sensing and monitoring bacteria [25][26][27][28] . Through translating variations of dielectric properties to quantifiable signals such has resonant amplitude and frequency, microwave sensing has shown to be adaptive towards the needs of biosensing and inexpensively implemented without the need to execute laborious protocols 25,27,29,30 .…”

Section: Introductionmentioning

confidence: 99%

Rapid Antibiotic Susceptibility Testing on Pathogenic Bacteria in Solid Growth Medium Using a Real-Time and Contactless Planar Microwave Resonator Sensor

Jain¹,

Pillai²,

Narang³

et al. 2021

Preprint

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Infection diagnosis and antibiotic susceptibility testing (AST) are pertinent clinical microbiology practices that are in dire need of improvement, as current standards are not able to keep up with the mutations and resistance development of certain bacterial strains. This paper presents a novel way to conduct AST which hybridizes disk diffusion AST with microwave resonators for rapid, contactless, non-invasive and high-throughput testing. This work uses Escherichia coli (E. coli) cultured on solid agar and places bacteria samples on a microwave split-ring resonator along with antibiotic disks (erythromycin) of various doses to demonstrate the viability of this sensing method in a clinical microbiological setting. The microwave resonator, operating at a 1.76 GHz resonant frequency, boasted a 5 mm2 sensitive sensing region. A one-port sensor was designed and optimized for detecting dielectric property variations of lossy dielectric materials accurately. This sensor was calibrated to detect uninhibited growth of the bacteria at 0.005 dB/hr, with a maximum change of 0.07 dB over the course of 15 hrs. The transient resonant amplitude change was subsequently dampened for each increasing dosage of antibiotic tested, with 45 µg of erythromycin showing negligible change indicating complete inhibited growth. This AST sensor demonstrated decisive results of antibiotic susceptibility in under 6 hours and shows great promise to further automate the intricate workflow of AST in clinical settings, while providing rapid, sensitive, non-invasive and high-throughput detection capabilities.

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Rapid and real-time monitoring of bacterial growth against antibiotics in solid growth medium using a contactless planar microwave resonator sensor

Jain

Nadaraja

Narang

et al. 2021

Sci Rep

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Infection diagnosis and antibiotic susceptibility testing (AST) are pertinent clinical microbiology practices that are in dire need of improvement, due to the inadequacy of current standards in early detection of bacterial response to antibiotics and affordability of contemporarily used methods. This paper presents a novel way to conduct AST which hybridizes disk diffusion AST with microwave resonators for rapid, contactless, and non-invasive sensing and monitoring. In this research, the effect of antibiotic (erythromycin) concentrations on test bacterium, Escherichia coli (E. coli) cultured on solid agar medium (MH agar) are monitored through employing a microwave split-ring resonator. A one-port microwave resonator operating at a 1.76 GHz resonant frequency, featuring a 5 mm2 sensitive sensing region, was designed and optimized to perform this. Upon introducing uninhibited growth of the bacteria, the sensor measured 0.005 dB/hr, with a maximum change of 0.07 dB over the course of 15 hours. The amplitude change decreased to negligible values to signify inhibited growth of the bacteria at higher concentrations of antibiotics, such as a change of 0.005 dB in resonant amplitude variation while using 45 µg of antibiotic. Moreover, this sensor demonstrated decisive results of antibiotic susceptibility in under 6 hours and shows great promise to expand automation to the intricate AST workflow in clinical settings, while providing rapid, sensitive, and non-invasive detection capabilities.

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Microwave sensor for detection of E. coli in water

Cited by 7 publications

References 6 publications

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

Rapid Antibiotic Susceptibility Testing on Pathogenic Bacteria in Solid Growth Medium Using a Real-Time and Contactless Planar Microwave Resonator Sensor

Rapid and real-time monitoring of bacterial growth against antibiotics in solid growth medium using a contactless planar microwave resonator sensor

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