Microwave plasma-assisted silicon nanoparticles: cytotoxic, molecular, and numerical responses against cancer cells

Wahab, Rizwan; Khan, Farheen; Gupta, Anoop; Wiggers, Hartmut; Saquib, Quaiser; Faisal, Mohammad; Ansari, Sabiha M.

doi:10.1039/c8ra10185j

Cited by 9 publications

(3 citation statements)

References 71 publications

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“…Microwave plasma-assisted silicon nanoparticles were shown to be cytotoxic and to have molecular and numerical responses against cancer cells 54 . Silver nanoparticles and earthworm combinations mediated anti-proliferative activity at increasing concentration as revealed by DNA analysis of Hep-G2 cells 55 , 56 . In accordance to our present findings were Amorphophallus paeoniifolius peels ZnO NP inducing cancer cell apoptosis at higher concentrations 57 .…”

Section: Discussionmentioning

confidence: 99%

Zinc oxide nanoparticles using plant Lawsonia inermis and their mosquitocidal, antimicrobial, anticancer applications showing moderate side effects

Amuthavalli

Hwang

Dahms‬

et al. 2021

Sci Rep

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Microbes or parasites spread vector-borne diseases by mosquitoes without being affected themselves. Insecticides used in vector control produce a substantial problem for human health. This study synthesized zinc oxide nanoparticles (ZnO NPs) using Lawsonia inermis L. and were characterized by UV–vis, FT-IR, SEM with EDX, and XRD analysis. Green synthesized ZnO NPs were highly toxic against Anopheles stephensi, whose lethal concentrations values ranged from 5.494 ppm (I instar), 6.801 ppm (II instar), 9.336 ppm (III instar), 10.736 ppm (IV instar), and 12.710 ppm (pupae) in contrast to L. inermis treatment. The predation efficiency of the teleost fish Gambusia affinis and the copepod Mesocyclops aspericornis against A. stephensi was not affected by exposure at sublethal doses of ZnO NPs. The predatory potency for G. affinis was 45 (I) and 25.83% (IV), copepod M. aspericornis was 40.66 (I) and 10.8% (IV) while in an ZnO NPs contaminated environment, the predation by the fish G. affinis was boosted to 71.33 and 34.25%, and predation of the copepod M. aspericornis was 60.35 and 16.75%, respectively. ZnO NPs inhibited the growth of several microbial pathogens including the bacteria (Escherichia coli and Bacillus subtilis) and the fungi (Alternaria alternate and Aspergillus flavus), respectively. ZnO NPs decreased the cell viability of Hep-G2 with IC50 value of 21.63 µg/mL (R2 = 0.942; P < 0.001) while the concentration increased from 1.88 to 30 µg/mL. These outcomes support the use of L. inermis mediated ZnO NPs for mosquito control and drug development.

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Section: Discussionmentioning

confidence: 99%

Zinc oxide nanoparticles using plant Lawsonia inermis and their mosquitocidal, antimicrobial, anticancer applications showing moderate side effects

Amuthavalli

Hwang

Dahms‬

et al. 2021

Sci Rep

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“…On the other hand, the FESEM images of all the nanoparticles have shown the size <50nm of all the nanoparticles. Whereas DLS has shown that most amorphous nanoparticles because of rapidly agglomeration due to their high surface area and reactivity, resulting in differences in primary and secondary size (27,28). So that particle size reached above 1000 nm, whereas for B-E samples, the mean particle size was <50 nm.…”

Section: Characterization Of Sio 2 Npsmentioning

confidence: 99%

WITHDRAWN: The effect of crystallization level of silica nanoparticles on cell proliferation of MRC-5 cell line and its prediction using artificial neural network

Abbasi

Hashemi

et al. 2020

Preprint

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Background: This study investigated the effect of the level of silica nanoparticles (SiO2NPs) crystallization on the cell proliferation of MRC-5 cells and its prediction using an artificial neural network (ANN).Methods: Variables studied included temperature (70-1000°C), calcination time (2, 12 and 24 hours), and catalyst feed rate (0.01, 0.05 and 0.1mL/min). Cell proliferation was determined by the MTT test after 24 hours of exposure, and results were analyzed using the t-test in MATLAB.Results: the synthesized particles size was less than 50nm, and the XRD peak varied from 30 to 21° during the increase in calcination temperature. The maximum level of crystallization was at 800°C (58% relative to amorphous) with the lowest cell viability. Cell proliferation decreased with increasing concentration of nanoparticles (p<0.05) and increasing feed rate. There was also a positive relationship between increased crystallization and decreased cell proliferation (R2=0.78), but no such association was observed for calcination time. Cell proliferation of MRC-5 was slightly correlated with the linear regression model (MSE>0.12), while ANN was well predicted by the Levenberg–Marquardt algorithm. The suggested structure in this study was 4:10:1 with R2all=0.97, R2test=0.97, RMSE=0.25 and MSE=0.003. The correlation between laboratory results and ANN prediction was 0.94, and the minimum and maximum OD level in the laboratory data and predicted ANN were attributed to 20 and 13 runs.Conclusion: changes in the degree of crystallization of SiO2NPs, an increase in concentration, and the rate of catalyst feed during crystallization of SiO2NPs were practical factors in increasing cytotoxicity.

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“…The electrostatic interaction between the nanocomposite and the cell lines may have caused a reduction in the cytotoxicity effects, resulting in a lower rate of death with extended incubation time (32).The nonspeci c surface reactivity had a hatching rate that was proportional to the concentration. The CeO 2 /ZnO/GO hybrid nanocomposite demonstrated stronger cytotoxicity effects against HeLa cells than previously reported CeO 2 nanoparticles (33,34) when tested for anticancer activities.The %cell inhibition rate induced by interacting cerium oxide nanoparticles, zinc oxide NPs and the hybrids against various cancer cell lines was compared to previously published results and is summarized in Table 1. The induced % cell death upon interaction of CeO 2 /ZnO/GO hybrid with HeLa cell line was further subject to qualitative analysis using AO/EB Dual Staining study which was performed at different concentrations such as 1000µg/ml, 500µg/ml and 250µg/ml.…”

Section: In-vitro Cytotoxicity Evaluation Of Ceo2/zno/go and Ceo2/cddp-zno/go Systemson Hela Cells And Normal Cellsmentioning

confidence: 62%

Evaluation of Cytotoxicity and Apoptotic behavior of Cerium Oxide/Zinc Oxide/ Graphene Oxide in HeLa and VERO cell Lines

Saranya¹,

Sreeja²,

Arivanandan

et al. 2021

Preprint

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Using the ultrasonic approach, we produced a morphology involving cerium oxide/ Zinc oxide/graphene oxide (CeO2/ZnO/GO) nanocomposite-based system. The developed nanocomposite was examined using X-Ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FESEM). The average crystallite size was found to be 11.44 nm, as determined by XRD. FTIR analysis was used to confirm the existence of functional groups. FESEM was used to verify the morphological properties of CeO2/ZnO/GO. The micromorphology of CeO2/ZnO/GO nanocomposite reveals a smoother sheet-like structure. In addition, using an antiproliferative assay test, the developed nanosystem was evaluated for its scavenging anti-cancer capability against HeLa cell lines at various doses and incubation intervals. In our investigation, the effective IC50 concentration was reported to be 62.5 µg/ml at 72 h. Further, the developed nanosystem was evaluated for its killing efficacy against normal cell line. To identify apoptosis-associated alterations of cell membranes throughout the apoptosis process, a dual acridine orange/ethidium bromide (AO/EB) fluorescent staining was done using CeO2/ZnO/GO nanocomposite at three specific concentrations. The quantitative analysis was carried out using flow cytometry (FACS study) to determine the cell cycle during which the greatest number of HeLa cells were destroyed. According to the results of the FACS investigation, maximum cell cycle has taken place in P2, P4.As a result, the newly designed CeO2/ZnO/GO hybrid has demonstrated improved anti-cancer efficacy against the HeLa cell line, making it a better therapeutic agent for cervical cancer detection.

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Microwave plasma-assisted silicon nanoparticles: cytotoxic, molecular, and numerical responses against cancer cells

Abstract: Silicon nanoparticles (SiNPs), which have a special place in material science due to their strong luminescent property and wide applicability in various physicochemical arenas synthesised via a microwave plasma-assisted process using an argon–silane mixture.

Cited by 9 publications

References 71 publications

Zinc oxide nanoparticles using plant Lawsonia inermis and their mosquitocidal, antimicrobial, anticancer applications showing moderate side effects

Zinc oxide nanoparticles using plant Lawsonia inermis and their mosquitocidal, antimicrobial, anticancer applications showing moderate side effects

WITHDRAWN: The effect of crystallization level of silica nanoparticles on cell proliferation of MRC-5 cell line and its prediction using artificial neural network

Evaluation of Cytotoxicity and Apoptotic behavior of Cerium Oxide/Zinc Oxide/ Graphene Oxide in HeLa and VERO cell Lines

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