Microwave irradiation for fixation and immunostaining of endothelial cells in situ

Katoh, Kazuo; Kano, Yumiko; Ookawara, Shigeo

doi:10.1080/10520290902879763

Cited by 7 publications

(10 citation statements)

References 29 publications

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“…The above protocol is applicable for staining of cryosections (see also [42]). See also immunofluorescence microscopy of paraffin-embedded samples by other authors [35, 37].…”

Section: Application For Immunofluorescence Microscopy [35 37 42mentioning

confidence: 99%

“…Samples were observed by confocal laser scanning microscopy, and focus was adjusted at the endothelial cell layer. Bar = 10 μ m. See also [42]. …”

Section: Figurementioning

confidence: 99%

“…Microwave irradiation is also applied for fluorescence in situ hybridization (FISH) analysis of paraffin-embedded tissues [39–41]. Recently, the author described microwave-irradiated blood vessel fixation and immunofluorescence microscopy [42]. In this case, microwave irradiation was used to increase penetration of fixatives.…”

Section: Introductionmentioning

confidence: 99%

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Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining

Katoh

2016

International Journal of Cell Biology

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Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions. Minimizing the incubation time in fixative reduces disruption of tissue morphology, and reducing the incubation time in staining solution or antibody solution decreases nonspecific labeling. Reduction of incubation time in staining solution also decreases the level of background noise. Microwave-assisted tissue preparation is applicable for tissue fixation, decalcification of bone tissues, treatment of adipose tissues, antigen retrieval, and other special staining of tissues. Microwave-assisted tissue fixation and staining are useful tools for histological analyses. This review describes the protocols using microwave irradiation for several essential procedures in histochemical studies, and these techniques are applicable to other protocols for tissue fixation and immunostaining in the field of cell biology.

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“…The above protocol is applicable for staining of cryosections (see also [42]). See also immunofluorescence microscopy of paraffin-embedded samples by other authors [35, 37].…”

Section: Application For Immunofluorescence Microscopy [35 37 42mentioning

confidence: 99%

“…Samples were observed by confocal laser scanning microscopy, and focus was adjusted at the endothelial cell layer. Bar = 10 μ m. See also [42]. …”

Section: Figurementioning

confidence: 99%

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Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining

Katoh

2016

International Journal of Cell Biology

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“…All animals were anesthetized with an overdose of sodium pentobarbital administered by intraperitoneal injection. After perfusion with 0.85% NaCl containing 200 units of heparin sodium via the left ventricle, the abdominal aortae were excised and fixed with 1% or 2% paraformaldehyde using the microwave irradiation method [26]. …”

Section: Methodsmentioning

confidence: 99%

“…After permeabilization with 0.5% Triton X-100 in PBS for 5 min, specimens were incubated with antibodies; some specimens were double-stained with rhodamine-labeled phalloidin. Some specimens were stained using microwave irradiation method [26, 27]. Specimens were mounted in 90% glycerol in PBS containing 2.5% 1,4-diazabicyclo[2.2.2]octane (DABCO; Sigma) and examined by confocal laser scanning microscopy (CLSM) (FV-1000; Olympus, Tokyo, Japan) with a Plan Apochromat ×60 (N.A.…”

Section: Methodsmentioning

confidence: 99%

Distribution of Cytoskeletal Components in Endothelial Cells in the Guinea Pig Renal Artery

Katoh

Noda

2012

International Journal of Cell Biology

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The cytoskeletal components of endothelial cells in the renal artery were examined by analysis of en face preparations under confocal laser scanning microscopy. Renal arterial endothelial cells were shown to be elongated along the direction of blood flow, while stress fibers ran perpendicular to the flow in the basal portion. Focal adhesions were observed along the stress fibers in dot-like configurations. On the other hand, stress fibers in the apical portion of cells ran along the direction of flow. The localizations of stress fibers and focal adhesions in endothelial cells in the renal artery differed from those of unperturbed aortic and venous endothelial cells. Tyrosine-phosphorylated proteins were mainly detected at the sites of cell-to-cell apposition, but not in focal adhesions. Pulsatile pressure and fluid shear stress applied over endothelial cells in the renal artery induce stress fiber organization and localization of focal adhesions. These observations suggest that the morphological alignment of endothelial cells along the direction of blood flow and the organization of cytoskeletal components are independently regulated.

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Liposomes Under Shear: Structure, Dynamics, and Drug Delivery Applications

Karaz

Şenses

2023

Advanced NanoBiomed Research

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The targeted delivery to specific locations while not causing damage to healthy tissues efficiently remains a challenge in drug delivery systems. Through addressing this issue, stimuli‐responsive materials have been under investigation. As one of the fundamental forces associated with blood flow, shear stress is taken as an advantage to design shear‐sensitive drug carriers. Although blood flow is modeled as laminar flow under normal conditions, in case of constrictions caused by endothelial shear stress, cardiovascular diseases, or angiogenesis due to tumor formation, local shear stress can dramatically increase. To date, shear‐sensitive materials have been investigated under two main categories: shear‐disaggregated and shear‐deformed nanoparticles based on their structural mechanism after exposure to high‐shear stress. Among them, liposomes are promising materials with their soft and deformable structure, high biocompatibility, controlled‐release properties, and sensitivity to shear stress. Herein, in this review, the effects of shear stress on liposomes in terms of their structural changes, flow regimes, rheological properties, and drug delivery applications are discussed. It is believed that this work provides a basis for designing more effective drug delivery systems considering the complexity of the human body.

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Microwave irradiation for fixation and immunostaining of endothelial cells in situ

Cited by 7 publications

References 29 publications

Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining

Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining

Distribution of Cytoskeletal Components in Endothelial Cells in the Guinea Pig Renal Artery

Liposomes Under Shear: Structure, Dynamics, and Drug Delivery Applications

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