Microwave Irradiation Affects Gene Expression in Plants

Vian, Alain; Roux, David; Girard, Sébastien; Bonnet, Pierre; Paladian, Françoise; Davies, Eric; Ledoigt, Gérard

doi:10.4161/psb.1.2.2434

Cited by 75 publications

(86 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[2][3] This proposal is supported by numerous experimental results. [4][5][6][7][8][9][10][11][12] The response of plants to stimuli involves changes in the level of free calcium in the cytosol and such changes exhibit complex spatio-temporal dynamics. [13][14][15][16][17][18][19][20] Using the model of the induction of epidermal meristems in the hypocotyl of flax seedlings, [7][8][9][10][11] our group has shown that when flax seedlings (a few days in age) were subjected to both an abiotic stimulus and a 1 to 3 day-long depletion of calcium in the nutrient medium, they developed numerous meristems over the following fortnight.…”

Section: Introductionmentioning

confidence: 99%

Pharmacological Evidence for Calcium Involvement in the Long-Term Processing of Abiotic Stimuli in Plants

Verdus

Sceller

Norris

et al. 2007

Plant Signaling & Behavior

View full text Add to dashboard Cite

AbbReViATioNSEDTA ethylenediaminetetraacetic acid EGTA ethylene glycol tetraacetic acid RR ruthenium red SCT slow cold treatment AbSTRACTInformation about abiotic conditions is stored for long periods in plants and, in flax seedlings, can lead to the production of meristems. To investigate the underlying mechanism, flax seedlings were given abiotic stimuli that included a mechanical stimulus (by manipulation), one or two cold shocks, a slow cold treatment and a drought stress and, if these seedlings were then subjected to a temporary (1 to 3 days) depletion of calcium, epidermal meristems were produced in the seedling hypocotyls. This production was inhibited by the addition to the nutrient media of EGTA, ruthenium red, lanthanum or gadolinium that affect calcium availability or calcium transport. Use of these agents revealed a period of vulnerability in information processing that was less than two min for mechanical stimuli and over five min for other abiotic stimuli, consistent with information about mechanical stimuli being stored particularly fast. We propose that external calcium is needed for the transduction/storage of the information for meristem production whilst a temporary depletion of external calcium is needed for the actual production of meristems. Such roles for calcium would be consistent with a mechanism based on ion condensation on charged polymers.

show abstract

Section: Introductionmentioning

confidence: 99%

Pharmacological Evidence for Calcium Involvement in the Long-Term Processing of Abiotic Stimuli in Plants

Verdus

Sceller

Norris

et al. 2007

Plant Signaling & Behavior

View full text Add to dashboard Cite

show abstract

“…The EMF-induced changes of the transcript level of cell cycle regulatory and apoptosis-related genes were shown [80,81]. The 3.5 fold upregulation of mRNA stress-related transcription factor bZIP after exposure to 900 MHz microwaves was shown [82]. After the exposure of human lens epithelial cells to 1800-MHz GSM-like radiation for 2 h (SAR -1.0, -3.5 W/kg) four proteins were upregulated.…”

Section: The Action Of Emf On Intracellular Regulatory Systemsmentioning

confidence: 96%

The Main Approaches of Studying the Mechanisms of Action of Artificial Electromagnetic Fields on Cell

Shckorbatov¹

2014

J Elec Electron Syst

View full text Add to dashboard Cite

“…Although the research programs concerned by in vitro experiments led under investigation various types of exposure systems [16], the MSRC were very seldom used [2]. Previously, we identified a formal link between an electromagnetic environment (900 MHz) and the response of a plant model [17][18][19][20] from MSRC experiments. However, the interest of this exposure device for in vitro experiments is highlighted here in the case of human skin cells in culture.…”

Section: Exposure Setups For Bioelectromagnetic Experiments In High Fmentioning

confidence: 99%

Mode Stirred Reverberation Chamber (Msrc): A Large and Efficient Tool to Lead High Frequency Bioelectromagnetic in Vitro Experimentation

Lalléchère¹,

Girard²,

Roux³

et al. 2010

PIER B

View full text Add to dashboard Cite

Abstract-An important aspect of the studies undertaken in bioelectromagnetism relates to the choice of exposure facility. The characteristics of a real electromagnetic environment are far more complex compared to the one plane wave irradiation set-up used in the majority of bioelectromagnetic studies. Moreover, biological requirements should represent the starting point in the design of an in vitro exposure system. Indeed it is important to avoid altering the electromagnetic properties of the exposure system in the presence of biological equipments. Related to these two essential points, this article contributes to show the advantages of a Mode Stirred Reverberation Chamber (MSRC) to guarantee a controlled electromagnetic environment around biological materials for in vitro experimentation. An example of irradiation of in vitro human skin cells cultures is considered to illustrate this paper. In order to show that the biological conditions and the physical requirements for in vitro experiments are checked, two aspects are described. Firstly we achieved the characterization of the electromagnetic field generated around the biological system (both equipments and cultures). Secondly the analysis of the Specific Absorption Rate (SAR) inside the biological medium was evaluated both numerically and experimentally. Initially, the statistical properties of fields inside the MSRC were checked with or without the biological devices in order to verify their electromagnetic transparency with respect to the reverberating properties of the electromagnetic environment (inside MSRC). We checked the good agreement of the experimental electromagnetic power distribution with the theoretical one.The computation of electromagnetic energy absorbed by biological medium (SAR) was based upon Finite Difference in Time Domain (FDTD) technique. A numerical analogy was achieved between the MSRC behavior and a free-space finite sum of random plane waves. Simulations are able to provide both an estimation of SAR distribution inside each biological culture dish and a computation of the coupling effects between dishes. Relying on the previous conclusions, temperature measurements were led to evaluate the experimental SAR levels and its time variations inside the MSRC. Two high-frequency (900 MHz) environments were considered: A 10 minutes exposure with field amplitude inside the biological incubator of 7.87 V/m and 30 minutes with 41 V/m (SAR ranged from 2.6 mW/kg to 73 mW/kg, mean values). Numerical and experimental results prove the ability of MSRC to provide a large and efficient tool to achieve bioelectromagnetic experiments at high frequencies.

show abstract

Microwave Irradiation Affects Gene Expression in Plants

Cited by 75 publications

References 20 publications

Pharmacological Evidence for Calcium Involvement in the Long-Term Processing of Abiotic Stimuli in Plants

Pharmacological Evidence for Calcium Involvement in the Long-Term Processing of Abiotic Stimuli in Plants

The Main Approaches of Studying the Mechanisms of Action of Artificial Electromagnetic Fields on Cell

Mode Stirred Reverberation Chamber (Msrc): A Large and Efficient Tool to Lead High Frequency Bioelectromagnetic in Vitro Experimentation

Contact Info

Product

Resources

About