Microwave dielectric relaxation & polarization study of binary mixture of methylethylketone with nitrobenzene

Abstract

Present paper reveals detailed study of dielectric relaxation and dielectric polarization and physicochemical study of binary polar-polar liquid mixture i.e. dielectric constant, relaxation time, viscosity, density of methylethylketone (MEK) with nitrobenzene (NB) at 303 K. The measured dielectric and physicochemical parameters employed to acquire additional derived properties like Bruggeman factor, molar refraction and excess properties like static dielectric constant, excess inverse relaxation time, excess molar volume, excess viscosity, excess molar refraction, Gibbs free energy, and enthalpy of activation. The variation of this parameters with composition of these quantities has been used to explain the type, strength and nature of intermolecular interactions between MEK+NB binary mixture. Attained results authenticate that there are strong hydrogen-bond interactions between unlike molecules of different groups of MEK+NB mixtures and that 1:1 complexes are produced and strength of intermolecular interaction rises with rise in concentration of MEK.

Theoretical Investigation into the Change in the Number of Water Molecules in Solvent Inaccessible Region of an Enzyme and Enzyme-substrate Complex

“…(2) and Eq. (8). A careful examination of the equations reveals that the slope and intercept may not be positive or negative; the values depend on the magnitude of the ratio given as ‫ܭ‬ positive slope or correlation and either a positive intercept or intercept which is negative but much smaller in magnitude.…”

“…Other forces are hydrophobic forces; the latter is relevant for the aggregation of hydrophobic moieties; it can also enhance protein folding. It is known elsewhere [8] that hydrogen bonding occurs in binary mixtures of organic solutes such as ethanol and sucrose in this research. There could be altered dielectric property of the primary solvent, water that can influence changes in the conformational stability of the enzyme.…”

“…(8). Equation (8) which arises from theoretical exposition of Buurma et al [8] represented the first time observation was made of the appearance of variable -concentration of cosolute -in two places as an independent variable in an equation. Soluble polar organic substances called osmolytes may be excluded from the protein surface domain on account of their inability to penetrate protein's inner region.…”