Microwave Dielectric Absorption Spectroscopy Aiming at Novel Dosimetry Using DNAs

Izumi, Yoshinobu; Hirayama, Makoto; Matuo, Youichirou; Sunagawa, Takeyoshi

doi:10.14407/jrpr.2017.42.1.21

Cited by 1 publication

(1 citation statement)

References 2 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous paper, plasmid DNA irradiated with gamma-rays is measured using resonance frequency [10]. The resonance frequency shifted to lower frequency with an increase in absorbed dose.…”

Section: Jrprmentioning

confidence: 99%

Evaluation of DNA Damage Using Microwave Dielectric Absorption Spectroscopy

Hirayama

Matuo

Sunagawa

et al. 2016

Journal of Radiation Protection and Research

Self Cite

View full text Add to dashboard Cite

Background: Evaluation of deoxyribonucleic acid (DNA)-strand break is important to elucidate the biological effect of ionizing radiations. The conventional methods for DNA-strand break evaluation have been achieved by Agarose gel electrophoresis and others using an electrical property of DNAs. Such kinds of DNA-strand break evaluation systems can estimate DNAstrand break, according to a molecular weight of DNAs. However, the conventional method needs pre-treatment of the sample and a relatively long period for analysis. They do not have enough sensitivity to detect the strand break products in the low-dose region. Materials and Methods: The sample is water, methanol and plasmid DNA solution. The plasmid DNA pUC118 was multiplied by using Escherichia coli JM109 competent cells. The resonance frequency and Q-value were measured by means of microwave dielectric absorption spectroscopy. When a sample is located at a center of the electric field, resonance curve of the frequency that existed as a standing wave is disturbed. As a result, the perturbation effect to perform a resonance with different frequency is adopted. Results and Discussion: The resonance frequency shifted to higher frequency with an increase in a concentration of methanol as the model of the biological material, and the Q-value decreased. The absorption peak in microwave power spectrum of the double-strand break plasmid DNA shifted from the non-damaged plasmid DNA. Moreover, the sharpness of absorption peak changed resulting in change in Q-value. We confirmed that a resonance frequency shifted to higher frequency with an increase in concentration of the plasmid DNA. Conclusion: We developed a new technique for an evaluation of DNA damage. In this paper, we report the evaluation method of DNA damage using microwave dielectric absorption spectroscopy.

show abstract

Section: Jrprmentioning

confidence: 99%