Microwave atomic force microscope: MG63 osteoblast-like cells analysis on nanometer scale

Abstract

combinations of proteins, carbohydrates, lipids and nucleic acids. These compositions carry out complicated biochemical and physiological process inside the cell. To study these complicated process, acquiring the morphological and fundamental function of these intracellular structures become an indispensable process.With the development of biotechnology, more and more approaches have been developed to investigate the intracellular structures. Immunofluorescence staining was the most widely used method to detect the changes of microfilaments in studying the behavior of cytoskeleton (Tumminia et al. 1998;Xu et al. 2011). Some cellular organelles such as chloroplast have been isolated from plant cells and their morphological structures and functions were investigated in the process of photosynthesis. In order to observe the intracellular structures, special microscopes have been designed. Tokita and cooperators have developed a new kind of microscope that was able to observe the internal structure of living cells (Matsuura-Tokita et al. 2006). Researchers from Caltech tried to observe the arrangement of individual proteins inside cells in a life-like state with the electron crymicroscope (Briegel et al. 2009). Transmission electron microscopy (TEM) and scanning electron microscope (SEM) also play important roles in cellular research.Advances in our understanding of molecular and cellular biology were and still are dictated by the development of new techniques, allowing the structural and functional study of living materials. The nano-scale surface analysis of microbial cells represents a significant challenge of current microbiology and is critical for developing new biotechnological and biomedical applications. The exploration of microbes using atomic force microscopy (AFM) is an exciting research field that has expanded rapidly in the past few years. AbstractIn this paper, we report a non-invasive and nondestructive probing method for analyzing the MG63 osteoblast-like cells. High frequency microwave atomic force microscope (M-AFM) can be used to measure the surface topography and microwave image of MG63 cells simultaneously in one scanning process. Under the frequency modulation AFM mode, the M-AFM probe tip can scan above the cell surface, maintaining a constant stand-off distance and the created lateral forces were small enough as not to sweep away or deform the fragile biomolecules. By analyzing the results, quantification such as, the number and distribution of organelles and proteins of MG63 cells as well as their dimension and electrical property information can be characterized. The unique potentials of that M-AFM imaging biological substrates with no damaging manner and nanometer scale resolution, while the original structure and function of the biomolecules during the investigation are preserved, make this technique very attractive to biologists.

Development of Silver Doped Hydroxyapatite Thin Films for Biomedical Applications

“…The results of the AFM studies are depicted in Figure 12a-f. The 2D AFM images highlighted that on the surface of the investigated thin films, typical patterns of cellular morphology of MG63 (ATCC CRL1427) cells having typical spindle-like shapes were found [49,50]. More than that, the data also suggested that the MG63 (ATCC CRL1427) cells had a good adherence to the samples and proliferated on the surfaces of the HAp, 0.05 AgHAp and 0.1 AgHAp thin films.…”

Positioning Technology of Tiny Electronic Components Based on Microscope and Computer Vision

“…It realizes the automatic loading and unloading of scanned samples through a manipulator. The AFM indirectly realizes the automatic replacement of the AFM scanning probe by replacing the probe holder equipped with the scanning probe [4].…”