Microwave-assisted synthesis and antioxidant activity of a hydrazone, (E)-1-(4-methoxybenzylidene)-2- phenylhydrazine

(E)-5-Benzyl-7-(3,4-dimethoxybenzylidene)-3-(3,4-dimethoxyphenyl)-2-phenyl-3,3a,4,5,6,7-hexahydro-2H-pyrazolo[4,3c] Pyridine

“…The antioxidant activity evaluation for the title compound was performed by following the previously reported literatures [18][19][20] with slight modification. First, 10 mg of the title compound were dissolved in 10 mL methanol (1000 µg/mL).…”

“…Then, as much as 80 µL of DPPH solution (40 µg/mL) were transferred to lines A-G, while line H was just filled with 130 µL of methanol as a blank and the test solutions were incubated for 30 min at room temperature in the dark room. The absorbance was measured at a wavelength of 517 nm with a 96-well microplate reader and the percentage of DPPH inhibition was calculated using formulae from previous reports [18][19][20]. The IC 50 value was calculated based on the linear regression equation (y = ax + b) from the curve by plotting the Ln concentration and the percentage of inhibition (see Supplementary Materials, Table S2 and Figure S34).…”

“…The IC 50 value was calculated based on the linear regression equation (y = ax + b) from the curve by plotting the Ln concentration and the percentage of inhibition (see Supplementary Materials, Table S2 and Figure S34). Then, the IC 50 value was categorized based on the previous reports [18][19][20].…”