MICROWAVE-ASSISTED SEED ESSENTIAL OIL OF ELEUTHEROCOCCUS SENTICOSUS AND ITS ANTIOXIDANT AND FREE RADICAL-SCAVENGING ACTIVITIES

Abstract

The aim of this study was to isolate the Eleutherococcus senticosus seed essential oil (ESEO) by an efficient microwave extraction method and to determine its antioxidant efficacy as reducing power, lipid peroxidation inhibition, as well as its ability to scavenge free radicals followed by determination of total phenolic content. The gas chromatography-mass spectroscopy analysis of ESEO revealed the presence of 21 different components representing 92.29% of the total oil. The ESEO evoked concentration-dependent reducing power and significant ferric ion-induced lipid peroxidation inhibition. The ESEO also showed significant antioxidant activity as inhibition of 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, superoxide and hydroxyl-free radicals by 71.72, 71.75, 72.87 and 72.36%, respectively. The ESEO yielded 6.02 Ϯ 0.01 mg/g gallic acid equivalent total phenolic content. The present study provides evidence that the ESEO may be considered as a potential source of natural antioxidants. PRACTICAL APPLICATIONSFree radicals play a very important role in food deterioration and disease progression. Plant-based essential oils have wide applications in food preservation, cosmetics and fragrance industry, mainly due to their wide margin of safety and broad range of antioxidant and other biological properties. In this study, the microwave-assisted seed essential oil from Eleutherococcus senticosus showed significant (P < 0.05) antioxidant and radical-scavenging activities in various experimental models including inhibition of lipid peroxidation and phenolic content. These findings confirm that E. senticosus has potential to be developed into dietary supplements and nutraceuticals.

Antioxidant and Antibacterial Properties of Essential Oil Extracted from an Edible SeaweedUndaria Pinnatifida

“…and a film thickness of 0.25 µm) using a standard procedure (Bajpai et al . ). The relative percentages of the chemical constituents of UPEO were expressed as the percentages of normalization of the peak area.…”

Antioxidant and Free Radical-Scavenging Potential of Essential Oil from E nteromorpha linza L. Prepared by Microwave-Assisted Hydrodistillation

“…Essential oils extracted from many plants and plant parts have been evaluated for their potential antioxidant properties (Gursoy et al 2012;Amorati et al 2013;Bajpai et al 2013;Pirbalouti et al 2013;Thusoo et al 2014). However, there have been no reports of the antioxidant capacities of essential oils from seaweeds.…”

Partially Purified Exopolysaccharide from Lactobacillus plantarum YML009 with Total Phenolic Content, Antioxidant and Free Radical Scavenging Efficacy

“…1 An aliquot (50 μL) of EPS (100 μg/mL) was mixed with 50 μL of 5% Folin-Ciocalteu reagent and the reaction mixture was incubated 25°C for 5 min in dark followed by addition of 100 μL of 20% Na 2 CO 3 solution. After incubation at room temperature for 20 min, the absorbance of the developed blue colored chromophore was measured at 730 nm against an appropriate blank solution.…”

“…1 Different concentrations of EPS (5-40 mg/mL) and reference compound ascorbic acid (25-200 μg/mL) were added to 0.004% methanolic solution of DPPH (1:1) in a 96-well microplate. The mixture was incubated at 37°C in dark for 30 min with shaking at 150 rpm.…”

“…1 A number of chronic human disorders including cancer, cardioand cerebral-vascular diseases have been recognized due to the possible consequence of ROS formation, and/or free radical damage to lipid, protein and nucleic acid. 2 A large number of functional EPS producing LAB have been isolated from various fermented sources which have shown their potent ability to act as antioxidants, probiotics, cholesterol lowering agents, and as a protecting agents against liver damage.…”