Microwave-assisted enzymatic hydrolysis to produce xylooligosaccharides from rice husk alkali-soluble arabinoxylan

Abstract

The prebiotic properties of xylooligosaccharides (XOS) and arabino-xylooligosaccharides (AXOS) produced from rice husk (RH) using microwave treatment combined with enzymatic hydrolysis were evaluated. The RH was subjected to microwave pretreatment at 140, 160 and 180 °C for 5, 10 and 15 min to obtain crude arabinoxylan (AX). Increasing microwave pretreatment time increased sugar content. Crude AX was extracted with 2% (w/v) sodium hydroxide at 25 °C for 24 h and used as a substrate for XOS production by commercial xylanases. Results showed that oligosaccharides produced by Pentopan Mono BG and Ultraflo Max provided xylobiose and xylotriose as the main products. AXOS was also present in the oligosaccharides that promoted growth of Lactobacillus spp. and resisted degradation by over 70% after exposure to simulated human digestion.

Microwave-assisted cassava pulp hydrolysis as food waste biorefinery for biodegradable polyhydroxybutyrate production

“…The hydrolysis of cassava pulp has been reported based on various approaches, such as acid hydrolysis ( Phowan and Danvirutai, 2014 ; Kurdi and Hansawasdi, 2015 ), enzymatic hydrolysis ( Bunterngsook et al, 2017 ; Siriwong et al, 2019 ), and a combination of acid and enzymatic hydrolysis ( Thongchul et al, 2010 ; Wattanagonniyom et al, 2017 ; Efeovbokhan et al, 2019 ; Areepak et al, 2022 ). Recently, microwave-assisted hydrolysis has gained some interest as it is an express and simple method for adopting biorefineries ( Tsubaki et al, 2008 ; Hermiati et al, 2012 ; Klangpetch et al, 2022 ). In this study, household microwaves were chosen and tested for their potential use in microwave-assisted hydrolysis strategies due to their price and easy operation.…”

The Potential of Xylooligosaccharides as Prebiotics and Their Sustainable Production from Agro-Industrial by-Products

“…Microwave treatment causes fragmentation and swelling, leading to the degradation of lignin and hemicellulose, thereby improving pentose yield. Results have shown that microwave pretreatment is a promising method for lignocellulosic degradation of rice husks [53]. Microwave-assisted reactions significantly decrease xylose content in hydrolysates and greatly increase the yield of XOs compared to conventional heating methods [54].…”