Microwave-assisted efficient conjugation of nanodiamond and paclitaxel

Hsieh, Yi Han; Liu, Kuang Kai; Sulake, Rohidas S.; Ji, Chao; Chen, Chinpiao

doi:10.1016/j.bmcl.2015.03.089

Cited by 11 publications

(5 citation statements)

References 19 publications

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“…Although noncovalent bonds have been widely used to couple bioactive molecules with ND [49][50][51][52], this method shows the disadvantage of low reproducibility and liability of the ND-molecule bond, which can be a result of nonhomogeneous ND surface chemistry combined with the low colloidal stability of ND. The surface functionalization of ND mainly with reactive carboxyl and hydroxyl groups brought the possibility of conjugation with a diversity of chemical groups or molecules (dyes, monomers, polymers, proteins, photosensitizers) [40,53,54], by covalent bond. Amongst the further chemical reaction that carboxylated ND can go through, the esterification outstands for providing increased colloidal stability of ND in aqueous suspension [40,[55][56][57].…”

Section: Discussionmentioning

confidence: 99%

Diamond Nanoparticles-Porphyrin mTHPP Conjugate as Photosensitizing Platform: Cytotoxicity and Antibacterial Activity

Hurtado

Cena

et al. 2021

Nanomaterials

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Conjugation of photosensitizers (PS) with nanoparticles has been largely used as a strategy to stabilize PS in the biological medium resulting in photosensitizing nanoparticles of enhanced photoactivity. Herein, (Meso-5, 10, 15, 20-tetrakis (3-hydroxyphenyl) phorphyryn (mTHPP) was conjugated with diamond nanoparticles (ND) by covalent bond. Nanoconjugate ND-mTHPP showed suitable stability in aqueous suspension with 58 nm of hydrodynamic diameter and Zeta potential of −23 mV. The antibacterial activity of ND-mTHPP was evaluated against Escherichia coli for different incubation times (0–24 h). The optimal activity was observed after 2 h of incubation and irradiation (660 nm; 51 J/cm2) performed right after the addition of ND-mTHPP (100 μg/mL) to the bacterial suspension. The inhibitory activity was 56% whereas ampicillin at the same conditions provided only 14% of bacterial growth inhibition. SEM images showed agglomerate of ND-mTHPP adsorbed on the bacterial cell wall, suggesting that the antimicrobial activity of ND-mTHPP was afforded by inducing membrane damage. Cytotoxicity against murine embryonic fibroblast cells (MEF) was also evaluated and ND-mTHPP was shown to be noncytotoxic since viability of cells cultured for 24 h in the presence of the nanoconjugate (100 μg/mL) was 78%. Considering the enhanced antibacterial activity and the absence of cytotoxic effect, it is possible to consider the ND-mTHPP nanoconjugate as promising platform for application in antimicrobial photodynamic therapy (aPDT).

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Section: Discussionmentioning

confidence: 99%

Diamond Nanoparticles-Porphyrin mTHPP Conjugate as Photosensitizing Platform: Cytotoxicity and Antibacterial Activity

Hurtado

Cena

et al. 2021

Nanomaterials

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“…The use of nanocarriers as drug delivery systems provide the opportunities to improve the efficacy of cancer therapy 44 – 46 . Various chemotherapeutic drugs can be linked to NDs 35 , 40 , 47 – 50 . ND-conjugated PTX has shown great promise in drug delivery and tumor blockage 35 , 40 , 41 .…”

Section: Discussionmentioning

confidence: 99%

“…Various chemotherapeutic drugs can be linked to NDs 35 , 40 , 47 – 50 . ND-conjugated PTX has shown great promise in drug delivery and tumor blockage 35 , 40 , 41 . In this study, ND-PTX effectively inhibited CRC cell growth in cellular and animal models.…”

Section: Discussionmentioning

confidence: 99%

“…The surface of NDs can be functionalized with chemicals or bio-molecules for various biomedical applications, including bio-labeling and drug delivery 30 – 39 . ND covalently linked with PTX was effective in drug delivery and tumor inhibition in human lung cancer 35 , 40 . In this study, the co-delivery of Cet and PTX by ND enhances the mitotic catastrophe and tumor inhibition in the EGFR-expressed CRC.…”

Section: Introductionmentioning

confidence: 99%

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Co-delivery of paclitaxel and cetuximab by nanodiamond enhances mitotic catastrophe and tumor inhibition

Lin

Raj

Liao

et al. 2017

Sci Rep

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The poor intracellular uptake and non-specific binding of anticancer drugs into cancer cells are the bottlenecks in cancer therapy. Nanocarrier platforms provide the opportunities to improve the drug efficacy. Here we show a carbon-based nanomaterial nanodiamond (ND) that carried paclitaxel (PTX), a microtubule inhibitor, and cetuximab (Cet), a specific monoclonal antibody against epidermal growth factor receptor (EGFR), inducing mitotic catastrophe and tumor inhibition in human colorectal cancer (CRC). ND-PTX blocked the mitotic progression, chromosomal separation, and induced apoptosis in the CRC cells; however, NDs did not induce these effects. Conjugation of ND-PTX with Cet (ND-PTX-Cet) was specifically binding to the EGFR-positive CRC cells and enhanced the mitotic catastrophe and apoptosis induction. Besides, ND-PTX-Cet markedly decreased tumor size in the xenograft EGFR-expressed human CRC tumors of nude mice. Moreover, ND-PTX-Cet induced the mitotic marker protein phospho-histone 3 (Ser10) and apoptotic protein active-caspase 3 for mitotic catastrophe and apoptosis. Taken together, this study demonstrated that the co-delivery of PTX and Cet by ND enhanced the effects of mitotic catastrophe and apoptosis in vitro and in vivo, which may be applied in the human CRC therapy.

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“…Skuteczność terapeutyczną PTX zwiększono opracowując nową metodę koniugacji wykorzystującą mikrofale, kwas stearynowy, trietyloaminę oraz chlorek metanosulfonylu. W porównawczym badaniu żywotności komórek gruczolakoraka płuc oraz raka jelita grubego, wykazano istotny wzrost śmiertelności komórek traktowanych koniugatem NDs-PTX utworzonym z wykorzystaniem mikrofal, w porównaniu z komórkami traktowanymi tym samym koniugatem syntetyzowanym klasycznymi metodami [126].…”

Section: Zastosowanie Nanodiamentów W Terapii Przeciwnowotworowejunclassified

Nanodiamenty: unikalne nanocząsteczki do zastosowania w biomedycynie i biotechnologii

Nowicki

Czarniewska

2020

Postepy Biochem

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Nanodiamenty to bardzo drobne cząstki diamentu, które ze względu na swój rozmiar w skali nano, unikalne właściwości fizykochemiczne oraz duży stosunek łatwo modyfikowalnej chemicznie powierzchni do objętości są interesujące dla biologów, chemików i fizyków. Praca ta stanowi przegląd literatury na temat ich przygotowania, właściwości i perspektyw związanych z możliwością ich zastosowania w naukach biomedycznych. Wysoka biokompatybilność nanodiamentów, potwierdzona przez szereg badań in vivo i in vitro, odróżnia je od innych nanocząstek i pozwala na ich zastosowanie jako neutralnego systemu transportu związków biologicznie czynnych. Takie nanocząstki można stosować jako nośniki peptydów, białek, kwasów nukleinowych, leków lub innych syntetycznych związków, które spowodują zamierzony efekt biologiczny w organizmie, w bioobrazowaniu i inżynierii tkankowej. Obiecujące wyniki badań na różnych modelach biologicznych sugerują praktyczne zastosowania rozwiązań nanotechnologicznych opartych na nanodiamentach.

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Microwave-assisted efficient conjugation of nanodiamond and paclitaxel

Cited by 11 publications

References 19 publications

Diamond Nanoparticles-Porphyrin mTHPP Conjugate as Photosensitizing Platform: Cytotoxicity and Antibacterial Activity

Diamond Nanoparticles-Porphyrin mTHPP Conjugate as Photosensitizing Platform: Cytotoxicity and Antibacterial Activity

Co-delivery of paclitaxel and cetuximab by nanodiamond enhances mitotic catastrophe and tumor inhibition

Nanodiamenty: unikalne nanocząsteczki do zastosowania w biomedycynie i biotechnologii

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