Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity

Lin, Yimo; Zeng, Huiyun; Wang, Ke; Lin, Hang; Li, Pingfan; Huang, Yuanxiang; Zhou, Shiyu; Zhang, Wei; Chao, Chen; Fan, Huajun

doi:10.1016/j.ijbiomac.2019.06.064

Cited by 81 publications

(27 citation statements)

References 53 publications

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“…The highest antioxidant parameters were noticed in the case of the control samples (23.53 ± 0.30 µmoles of TE/g dw for ABTS and 12.29 ± 0.87 µmoles of TE/g dw for FRAP). Antioxidant activity of L. edodes polysaccharides was reported before and this study confirms earlier findings [16,35,58]. Blanching caused a significant drop in FRAP test (7.59 ± 0.75 µmoles of TE/g dw), while no relevant change in ABTS parameter was noticed.…”

Section: Antioxidant Potentialsupporting

confidence: 92%

Processed Fruiting Bodies of Lentinus edodes as a Source of Biologically Active Polysaccharides

Ziaja‐Sołtys

Radzki

Nowak³

et al. 2020

Applied Sciences

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Water soluble polysaccharides (WSP) were isolated from Lentinus edodes fruiting bodies. The mushrooms were previously subjected to various processing techniques which included blanching, boiling, and fermenting with lactic acid bacteria. Therefore, the impact of processing on the content and biological activities of WSP was established. Non-processed fruiting bodies contained 10.70 ± 0.09 mg/g fw. Boiling caused ~12% decrease in the amount of WSP, while blanched and fermented mushrooms showed ~6% decline. Fourier transform infrared spectroscopy analysis (FTIR) confirmed the presence of β-glycosidic links, whereas due to size exclusion chromatography 216 kDa and 11 kDa molecules were detected. WSP exhibited antioxidant potential in FRAP (ferric ion reducing antioxidant power) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assays. Cytotoxic properties were determined on MCF-7 and T47D human breast cell lines using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) test. Both biological activities decreased as the result of boiling and fermenting.

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Section: Antioxidant Potentialsupporting

confidence: 92%

Processed Fruiting Bodies of Lentinus edodes as a Source of Biologically Active Polysaccharides

Ziaja‐Sołtys

Radzki

Nowak³

et al. 2020

Applied Sciences

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“…In comparison with the IC50 of other polysaccharides, the ability of Phyllanthus urinaria polysaccharides to scavenge DPPH free radicals was close to that of Rosa roxburghii Tratt. Polysaccharides (8.38 μg/mL) (Wang et al, 2018a), but higher than that of Chestnut rose polysaccharides (100 μg/mL) (Chen & Kan, 2018) and Lentinus edodes polysaccharides from tobacco waste (560 μg/mL) (Lin et al, 2019); the ability of Phyllanthus urinaria polysaccharides to scavenge ABTS free radicals was higher than that of Chimonobambusa quadrangularis Polysaccharides (452 μg/mL) (Chen et al, 2019a) and Kiwifruit Polysaccharides (1980 μg/mL) (Han et al, 2019), but lower than that of Pouteria campechiana seed Polysaccharides (29.9 μg/mL) (Ma et al, 2020).…”

Section: Antioxidant Activity Analysismentioning

confidence: 89%

Extraction optimization and antioxidant activity of Phyllanthus urinaria polysaccharides

Liu

2021

Food Sci. Technol

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Three extraction parameters including extraction time, material to solvent ratio, and extraction temperature for the extraction yield of Phyllanthus urinaria polysaccharides were optimized by response surface methodology. The results revealed that the optimal extraction process was extraction time of 5.3 h, extraction temperature of 93 °C, and material to solvent ratio of 1:47 g/mL, under which the polysaccharide yield was 6.40%; The three parameters had significant effect on the polysaccharide yield, and the influence of extraction time was the greatest and that of extraction temperature was the lowest. The antioxidant activity test indicated that the equivalent scavenging ability of Phyllanthus urinaria polysaccharides on DPPH and ABTS free radicals was 0.88 and 0.84 μg TBHQ/μg of polysaccharides, respectively; while the reducing power and total antioxidant capacity of Phyllanthus urinaria polysaccharides was equivalent to 93.62% and 93.55% of TBHQ, respectively. The Phyllanthus urinaria polysaccharides had good antioxidant activity.

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“…While the same concentration of DP‐3 had higher activity (50.12%) ( p < 0.05). The effect of natural active compounds/antioxidants on the scavenging of DPPH free radical is related to their hydrogen supply capacity, which indicated that the test sample DP fractions could donate hydrogen, which in turn exerted free radical scavenging activity (Lin et al., 2019).…”

Section: Resultsmentioning

confidence: 99%

Optimization of ultrasonic‐assisted extraction of polysaccharides from Hemerocallis citrina and the antioxidant activity study

Meng

Chen

et al. 2021

Journal of Food Science

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The present study discussed the optimization of the ultrasonic‐assisted extraction of polysaccharides from daylily polysaccharides (DPs). The extracted crude polysaccharides were further separated and purified, and the antioxidant activities including 1,1‐diphenyl‐2‐111 picrylhydrazyl (DPPH) radical scavenging, 2,2'‐azinobis‐(3‐ethylbenzthiazoline‐6‐sulphonate) (ABTS) radical scavenging, hydroxyl radical scavenging, and ferric‐reducing antioxidant power (FRAP) activities of the obtained fractions were also evaluated. The results showed that the optimal ultrasonic‐assisted extraction parameters with DPs yield of 15.25 ± 1.13% were water to powder ratio of 25 ml/g, extraction power of 694 W, extraction temperature of 71°C, extraction time of 38 min, and three times extraction. By DEAE Sepharose Fast Flow column, four water‐soluble polysaccharide fractions (DP‐1, DP‐2, DP‐3, and DP‐4) were successfully obtained. Monosaccharide component analysis showed that the four obtained fractions were all hetero‐polysaccharides that mainly contained rhamnose, arabinose, fructose, galactose, glucose, galacturonic acid, and glucuronic acid in different molar ratios. All the four DP fractions did show obvious antioxidant activities in vitro, and the DP‐3 component had relatively high ABTS free radical scavenging activity. Overall, our research showed that DPs could provide cheap raw materials for the development of natural antioxidants in medicines, functional foods, and even cosmetics. Practical Application This article deals with the optimization of the ultrasonic‐assisted extraction of polysaccharides from daylily and its antioxidant activities. The results showed that the optimal ultrasonic‐assisted extraction yield of DPs was 15.25 ± 1.13%. By DEAE Sepharose Fast Flow column, four water‐soluble polysaccharide fractions were successfully obtained, and all the four DP fractions did show obvious antioxidant activities in vitro. Daylily polysaccharides could provide cheap raw materials for the development of natural antioxidants in medicines, functional foods, and even cosmetics.

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Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity

Cited by 81 publications

References 53 publications

Processed Fruiting Bodies of Lentinus edodes as a Source of Biologically Active Polysaccharides

Processed Fruiting Bodies of Lentinus edodes as a Source of Biologically Active Polysaccharides

Extraction optimization and antioxidant activity of Phyllanthus urinaria polysaccharides

Optimization of ultrasonic‐assisted extraction of polysaccharides from Hemerocallis citrina and the antioxidant activity study

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