Microwave-assisted acid Digestion Procedure for Trace Elements Determination in PolychaeteChaetopterus variopedatus

Abstract

Procedimento de Digestão Assistido por Micro-ondas para Determinação de Elementos-traços no Poliqueta Chaetopterus variopedatusResumo: Este estudo avaliou e aplicou uma digestão, com ácido diluído, assistida por micro-ondas para determinar elementos-traço (As, Ba, Cd, Co, Cr, Cu, Hg, Mn, Ni e Sb) em amostras de poliquetas (Chaetopterus variopedatus). Após a otimização do método, empregando-se material certificado de referência (1566b CRM, tecido Oyster), os analitos foram determinados por espectrometria de massas com plasma indutivamente acoplado (ICP-MS) e espectrometria de emissão atômica com plasma indutivamente acoplado (ICP OES). A repetibilidade foi adequada (<10%), os resultados obtidos com o CRM concordaram com os valores certificados e o teor de carbono residual valores (RCC) foi menor do que 15%. Além disso, até dez amostras podem ser simultaneamente decompostas em 34 min., resultando num elevado rendimento. O método desenvolvido foi aplicado em amostras de poliquetas coletados na Baía de Todos os Santos, Bahia, Brasil. Palavras-chave:Elementos-traço; digestão assistida por micro-ondas; ácidos diluidos; tecido de poliquetas. AbstractThis study evaluated and applied a microwave-assisted diluted acid digestion to determine trace elements (As, Ba, Cd, Co, Cr, Cu, Hg, Mn, Ni and Sb) in polychaete (Chaetopterus variopedatus) samples. After the optimization of the method with certified reference material (CRM 1566b, Oyster tissue), the analytes were determined by inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP OES). Repeatability was adequate (< 10 %), results for CRM agreed with the certified values and the residual carbon content (RCC) values were lower than 15 %. Moreover, up to ten samples can be simultaneously decomposed in 34 min., resulting in a high sample throughput. The developed method was applied to polychaete samples collected from Todos os Santos Bay, Bahia, Brazil.

Development and Validation of an Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Method for Quantitative Analysis of Platinum in Plasma, Urine, and Tissues

“…For instance, Yang et al reported recoveries of Na, Al, K, V, Co, Zn, Se, Sr, Ag, Cd, Ni, and Pb between 90–110% determined by ICP-MS. 33 In another microwave-assisted acid digestion procedure for determination of metal contents in marine invertebrates, recoveries of 85% and 110% were reported for Cu and Ba, respectively. 34 …”

“…For instance, Yang et al reported recoveries of Na, Al, K, V, Co, Zn, Se, Sr, Ag, Cd, Ni, and Pb between 90-110% determined by ICP-MS. 33 In another microwaveassisted acid digestion procedure for determination of metal contents in marine invertebrates, recoveries of 85% and 110% were reported for Cu and Ba, respectively. 34 Low (1 ppb) and high (45 ppb) platinum quality control samples were used for assessing the freeze-thaw, shortterm stability, and long-term stability of the test samples in both rat urine and rat liver matrices. Results suggested that the samples were stable after three cycles of freezethaw and after short-and long-term storage for up to three months (Supplemental Material, Tables 6S-8S).…”