This study investigated the effect of pretreatment of orange and plantain
peels on the inactivation kinetics and thermodynamic properties of
polygalacturonase (PG) produced by Aspergillus awamori CICC 2040. Orange and
plantain peel powders were subjected to microwave-assisted NaOH pretreatment
and used as substrates for PG production. Un-treated peels served as
controls. The PG was purified using acetone precipitation and column
chromatography, and the inactivation kinetics, temperature dependency, and
thermodynamic properties of the crude and purified PGs were determined.
Higher inactivation rate constant was obtained for crude PG produced using
pretreated orange peel (CPOF) and plantain peel (CPPF) compared to PG
produced using untreated orange peel (Uo) and plantain peel (Up). At all the
temperatures considered, higher half-life and decimal reduction time were
recorded for CPOF and CPPF compared to Uo and Up. The highest half-life
(45.60 min) and decimal reduction time (151.49 min) were recorded for CPOF
at 60 ?C. Lower half-life and decimal reduction time were obtained for
purified PGs compared to the crude PG. Polygalacturonase produced from
pretreated peels had lower activation energy than those produced from
untreated ones. The higher activation energy was recorded for the PG
produced using orange peel compared to the one from plantain peels. The
enthalpy of CPOF and CPPF was slightly lower than Uo and Up. The
pretreatment of the peels resulted in a reduction of Gibbs free energy (?G )
and entropy (?S) of crude and purified PG. Higher ?G and ?S were recorded
for the purified PG compared to the crude PG. Negative entropy and enthalpy
were recorded for all the PGs. The findings from this study showed that the
kinetic and thermodynamic properties of PG, produced by Aspergillus awamori
CICC 2040, were enhanced by the pretreatment of orange and plantain peels.